Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Isolation and Characterization of Indigenous Marine Diatom Achnanthidium sp. BS-001 Producing a high Content of Omega-3 Fatty Acid and Fucoxanthin Production

오메가-3 지방산 및 푸코잔틴 고함량 토착 규조류 아크난티디움 균주의 분리 및 특성

  • Kim, Urim (Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • Cho, Dae-Hyun (Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • Heo, Jina (Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • Kim, Hee-Sik (Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
  • 김우림 (한국생명공학연구원 세포공장연구센터) ;
  • 조대현 (한국생명공학연구원 세포공장연구센터) ;
  • 허진아 (한국생명공학연구원 세포공장연구센터) ;
  • 김희식 (한국생명공학연구원 세포공장연구센터)
  • Received : 2019.05.28
  • Accepted : 2019.06.18
  • Published : 2019.06.30
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Abstract

Omega-3 fatty acids and carotenoids, which are known as representative high-value substances derived from microalgae, are being studied from various diatoms. Most of the diatoms contain fucoxanthin and omega-3 fatty acid. Fucoxanthin produced by diatom has been reported as bioactive compounds exhibiting strong antioxidant, anticancer and anti-inflammatory activities. However, the low growth rate and fucoxanthin content of diatoms are one of the big obstacles to the industrial application. In this study, indigenous marine diatom Achnanthidium sp. BS-001 was isolated for a candidate of fucoxanthin producer. Light intensity and temperature for the culture of Achnanthidium sp. BS-001 were optimized on PhotoBiobox. Optimization of silicate concentration for increasing BS-001 biomass productivity was confirmed in F/2 medium with various concentration of sodium silicate. As a result, condition of light intensity, temperature, and silicate concentration for optimal cultivation were $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, $18^{\circ}C$ and 0.106 mM, respectively. Maximum biomass productivity reaches to $154.3mg{\cdot}L^{-1}{\cdot}day^{-1}$, and then the content of omega-3 fatty acids and fucoxanthin were $19.4mg{\cdot}g^{-1}$, $9.05mg{\cdot}g^{-1}$, respectively. These results indicate that Achnanthidium sp. BS-001 has the potential to be used as a source of omega-3 fatty acids and fucoxanthin.

Keywords

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Figure 1. Morphological analysis (A) optical microscope and (B) electron microscope images

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Figure 2. Optimization of culture condition (A) light intensity (B) temperature

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Figure 3. Cultivation under various silicate concentration (A) cell density (B) specific growth rate and generation time and (C) final cell density and biomass productivity

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Figure 4. Lipid analysis of Achnanthidium sp. BS-001 (A) total lipid content, (B) nile red staining and (C) FAME composition

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Figure 5. Pigment analysis of Achnanthidium sp. (A) pigmen ts composition and (B) carotenoid composition

Table 1. Comparion of SFA, MUFA, and omerga fatty acids with other marine microalgae

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Table 2. Summary of biomass, omega-3 fatty acid and Fucoxanthin productivity

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Table 3. Comparison of total carotenoids and fucoxanthin content with other diatoms

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References

  1. Adarme-Vega, T.C., Thomas-Hall, S.R., Lim, D.K.Y. & Schenk, P.M. 2014. Effects of long chain fatty acid synthesis and associated gene expression in microalga Tetraselmis sp. Mar. Drugs. 12, 3381-98. https://doi.org/10.3390/md12063381
  2. Blich, E.G. & Dyer, W.J. 1959. Canadian Journal of Biochemistry and Physiology. Can. J. Biochem. Physiol. 37, 911-7. https://doi.org/10.1139/y59-099
  3. Cho, D.H., Choi, J.W., Kang, Z., Kim, B.H., Oh, H.M., Kim, H.S. & Ramanan, R. 2017. Microalgal diversity fosters stable biomass productivity in open ponds treating wastewater. Sci. Rep. 7, 1-11. https://doi.org/10.1038/s41598-016-0028-x
  4. Delbrut, A., Albina, P., Lapierre, T., Pradelles, R. & Dubreucq, E. 2018. Fucoxanthin and polyunsaturated fatty acids co-extraction by a green process. Molecules. 23, 1-15.
  5. Dyall, S.C. 2015. Long-chain omega-3 fatty acids and the brain: A review of the independent and shared effects of EPA, DPA and DHA. Front. Aging Neurosci. 7, 1-15. https://doi.org/10.3389/fnagi.2015.00001
  6. Foo, S.C., Yusoff, F.M., Ismail, M., Basri, M., Yau, S.K., Khong, N.M.H., Chan, K.W. et al. 2017. Antioxidant capacities of fucoxanthin-producing algae as influenced by their carotenoid and phenolic contents. J. Biotechnol. 241, 175-83. https://doi.org/10.1016/j.jbiotec.2016.11.026
  7. Garcia, J.L., de Vicente, M. & Galan, B. 2017. Microalgae, old sustainable food and fashion nutraceuticals. Microb. Biotechnol. 10, 1017-24. https://doi.org/10.1111/1751-7915.12800
  8. Gyeongje J. 2012. Algal flora of Korea. In: National Institute of Biological Resources (eds), Freshwater Diatoms V. vol 3 no. 7, National Institute of Biological Resources : Ministry of Environment, pp 13-15
  9. Heo, J., Cho, D.H., Ramanan, R., Oh, H.M. & Kim, H.S. 2015. PhotoBiobox: A tablet sized, low-cost, high throughput photobioreactor for microalgal screening and culture optimization for growth, lipid content and CO2 sequestration. Biochem. Eng. J. 103, 193-7. https://doi.org/10.1016/j.bej.2015.07.013
  10. Heo, J., Shin, D.S., Cho, K., Cho, D.H., Lee, Y.J. & Kim, H.S. 2018. Indigenous microalga Parachlorella sp. JD-076 as a potential source for lutein production: Optimization of lutein productivity via regulation of light intensity and carbon source. Algal Res.
  11. Mahabaleshwar V. Hegde, Anand Arvind Zanwar, Sharad P. Adekar. 2016. Omega-3 Fatty Acids: Keys to Nutritional Health, Humana Press, pp 109.
  12. Kim, S.M., Jung, Y.J., Kwon, O.N., Cha, K.H., Um, B.H., Chung, D. & Pan, C.H. 2012a. A potential commercial source of fucoxanthin extracted from the microalga Phaeodactylum tricornutum. Appl. Biochem. Biotechnol. 166, 1843-55. https://doi.org/10.1007/s12010-012-9602-2
  13. Kim, S.M., Kang, S.W., Kwon, O.N., Chung, D. & Pan, C.H. 2012b. Fucoxanthin as a major carotenoid in Isochrysis aff. galbana: Characterization of extraction for commercial application. J. Korean Soc. Appl. Biol. Chem. 55, 477-83. https://doi.org/10.1007/s13765-012-2108-3
  14. Peng, J., Yuan, J.P., Wu, C.F. & Wang, J.H. 2011. Fucoxanthin, a marine carotenoid present in brown seaweeds and diatoms: Metabolism and bioactivities relevant to human health. Mar. Drugs. 9, 1806-28. https://doi.org/10.3390/md9101806
  15. Opinion, S. 2010. Scientific Opinion on Dietary Reference Values for fats, including saturated fatty acids, polyunsaturated fatty acids, monounsaturated fatty acids, trans fatty acids, and cholesterol. EFSA J. 8, 1-107
  16. Sasser, M. 1987. Identification of bacteria by gas chromatography. Forum Mikrobiol. 10, 341-3.
  17. Simopoulos, A.P. 2003. Importance of the ratio of omega-6/omega-3 essential fatty acids: evolutionary aspects. World Rev. Nutr. Diet. 92, 1-22. https://doi.org/10.1159/000073788
  18. Tsai, H.P., Chuang, L. Te & Chen, C.N.N. 2016. Production of long chain omega-3 fatty acids and carotenoids in tropical areas by a new heat-tolerant microalga Tetraselmis sp. DS3. Food Chem. 192, 682-90. https://doi.org/10.1016/j.foodchem.2015.07.071
  19. Velasco, L.A., Carrera, S. & Barros, J. 2016. Isolation, culture and evaluation of Chaetoceros muelleri from the Caribbean as food for the native scallops, Argopecten nucleus and Nodipecten nodosus. Lat. Am. J. Aquat. Res. 44, 557-68. https://doi.org/10.3856/vol44-issue3-fulltext-14
  20. Ward, O.P. & Singh, A. 2005. Omega-3/6 fatty acids: Alternative sources of production. Process Biochem. 40, 3627-52. https://doi.org/10.1016/j.procbio.2005.02.020
  21. Xia, S., Wang, K., Wan, L., Li, A., Hu, Q. & Zhang, C. 2013. Production, characterization, and antioxidant activity of fucoxanthin from the marine diatom odontella aurita. Mar. Drugs. 11, 2667-81. https://doi.org/10.3390/md11072667
  22. Zhang, J., Sun, Z., Sun, P., Chen, T. & Chen, F. 2014. Microalgal carotenoids: Beneficial effects and potential in human health. Food Funct. 5, 413-25. https://doi.org/10.1039/c3fo60607d