한국해양바이오학회지 (Journal of Marine Bioscience and Biotechnology)

한국해양바이오학회 (The Korean Society for Marine Biotechnology)
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오메가-3 지방산 및 푸코잔틴 고함량 토착 규조류 아크난티디움 균주의 분리 및 특성

Isolation and Characterization of Indigenous Marine Diatom Achnanthidium sp. BS-001 Producing a high Content of Omega-3 Fatty Acid and Fucoxanthin Production

  • 김우림 (한국생명공학연구원 세포공장연구센터) ;
  • 조대현 (한국생명공학연구원 세포공장연구센터) ;
  • 허진아 (한국생명공학연구원 세포공장연구센터) ;
  • 김희식 (한국생명공학연구원 세포공장연구센터)
  • Kim, Urim (Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • Cho, Dae-Hyun (Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • Heo, Jina (Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • Kim, Hee-Sik (Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
  • 투고 : 2019.05.28
  • 심사 : 2019.06.18
  • 발행 : 2019.06.30
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초록

Omega-3 fatty acids and carotenoids, which are known as representative high-value substances derived from microalgae, are being studied from various diatoms. Most of the diatoms contain fucoxanthin and omega-3 fatty acid. Fucoxanthin produced by diatom has been reported as bioactive compounds exhibiting strong antioxidant, anticancer and anti-inflammatory activities. However, the low growth rate and fucoxanthin content of diatoms are one of the big obstacles to the industrial application. In this study, indigenous marine diatom Achnanthidium sp. BS-001 was isolated for a candidate of fucoxanthin producer. Light intensity and temperature for the culture of Achnanthidium sp. BS-001 were optimized on PhotoBiobox. Optimization of silicate concentration for increasing BS-001 biomass productivity was confirmed in F/2 medium with various concentration of sodium silicate. As a result, condition of light intensity, temperature, and silicate concentration for optimal cultivation were $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, $18^{\circ}C$ and 0.106 mM, respectively. Maximum biomass productivity reaches to $154.3mg{\cdot}L^{-1}{\cdot}day^{-1}$, and then the content of omega-3 fatty acids and fucoxanthin were $19.4mg{\cdot}g^{-1}$, $9.05mg{\cdot}g^{-1}$, respectively. These results indicate that Achnanthidium sp. BS-001 has the potential to be used as a source of omega-3 fatty acids and fucoxanthin.

키워드

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Figure 1. Morphological analysis (A) optical microscope and (B) electron microscope images

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Figure 2. Optimization of culture condition (A) light intensity (B) temperature

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Figure 3. Cultivation under various silicate concentration (A) cell density (B) specific growth rate and generation time and (C) final cell density and biomass productivity

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Figure 4. Lipid analysis of Achnanthidium sp. BS-001 (A) total lipid content, (B) nile red staining and (C) FAME composition

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Figure 5. Pigment analysis of Achnanthidium sp. (A) pigmen ts composition and (B) carotenoid composition

Table 1. Comparion of SFA, MUFA, and omerga fatty acids with other marine microalgae

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Table 2. Summary of biomass, omega-3 fatty acid and Fucoxanthin productivity

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Table 3. Comparison of total carotenoids and fucoxanthin content with other diatoms

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