치위생과학회지 (Journal of dental hygiene science)

  • 제19권2호
  • /
  • Pages.133-140
  • /
  • 2019
  • /
  • 1598-4478(pISSN)
  • /
  • 2233-7679(eISSN)
한국치위생과학회 (The Korean Society of Dental Hygiene Science)
권호 표지
DOI QR코드

DOI QR Code

Inflammatory Effect of Light-Emitting Diodes Curing Light Irradiation on Raw264.7 Macrophage

  • Jeong, Moon-Jin (Department of Oral Histology and Developmental Biology, School of Dentistry, Chosun University) ;
  • Kil, Ki-Sung (Department of Oral Histology and Developmental Biology, School of Dentistry, Chosun University) ;
  • Lee, Myoung-Hwa (Department of Oral Histology and Developmental Biology, School of Dentistry, Chosun University) ;
  • Lee, Seung-Yeon (Department of Oral Histology and Developmental Biology, School of Dentistry, Chosun University) ;
  • Lee, Hye-Jin (Department of Dental Hygiene, Dong-Pusan College) ;
  • Lim, Do-Seon (Department of Dental Hygiene, Graduate School of Public Health Science, Eulji University) ;
  • Jeong, Soon-Jeong (Department of Dental Hygiene, College of Health Science, Youngsan University)
  • 투고 : 2019.04.24
  • 심사 : 2019.05.22
  • 발행 : 2019.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Background: The light-emitting diode (LED) curing light used is presumed to be safe. However, the scientific basis for this is unclear, and the safety of LED curing light is still controversial. The purpose of this study was to investigate the effect of LED curing light irradiation according to the conditions applied for the polymerization of composite resins in dental clinic on the cell viability and inflammatory response in Raw264.7 macrophages and to confirm the stability of LED curing light. Methods: Cell viability and cell morphology of Raw264.7 macrophages treated with 100 ng/ml of lipopolysaccharide (LPS) or/and LED curing light with a wavelength of 440~490 nm for 20 seconds were confirmed by methylthiazolydiphenyl-tetrazolium bromide assay and microscopic observation. The production of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) was confirmed by NO assay and $PGE_2$ enzyme-linked immunosorbent assay kit. Expression of interleukin $(IL)-1{\beta}$ and tumor necrosis factor $(TNF)-{\alpha}$ in total RNA and protein was confirmed by reverse transcription polymerase chain reaction and Western blot analysis. Results: The LED curing light did not affect the viability and morphology of normal Raw264.7 cells but affected the cell viability and induced cytotoxicity in the inflammation-induced Raw264.7 cells by LPS. The irradiation of the LED curing light did not progress to the inflammatory state in the inflammation-induced Raw264.7 macrophage. However, LED curing light irradiation in normal Raw264.7 cells induced an increase in NO and $PGE_2$ production and mRNA and protein expression of $(IL)-1{\beta}$ and $(TNF)-{\alpha}$, indicating that it is possible to induce the inflammatory state. Conclusion: The irradiation of LED curing light in RAW264.7 macrophage may induce an excessive inflammatory reaction and damage oral tissues. Therefore, it is necessary to limit the long-term irradiation which is inappropriate when applying LED curing light in a dental clinic.

키워드

참고문헌

  1. Wataha JC, Lewis JB, Lockwood PE, Noda M, Messer RL, Hsu S: Response of THP-1 monocytes to blue light from dental curing lights. J Oral Rehabil 35: 105-110, 2008. https://doi.org/10.1111/j.1365-2842.2007.01806.x
  2. Wataha JC, Lockwood PE, Bouillaguet S, Noda M: In vitro biological response to core and flowable dental restorative materials. Dent Mater 19: 25-31, 2003. https://doi.org/10.1016/S0109-5641(02)00012-X
  3. Nakamura S, Mukai T, Senoh M: Candela‐class highbrightness InGaN/AlGaN double‐heterostructure blue‐lightemitting diodes. Appl Phys Lett 64: 1687-1689, 1994. https://doi.org/10.1063/1.111832
  4. Kurachi C, Tuboy AM, Magalhaes DV, Bagnato VS: Hardness evaluation of a dental composite polymerized with experimental LED-based devices. Dent Mater 17: 309-315, 2001. https://doi.org/10.1016/S0109-5641(00)00088-9
  5. Ergun G, Egilmez F, Cekic-Nagas I: The effect of light curing units and modes on cytotoxicity of resin-core systems. Med Oral Patol Oral Cir Bucal 15: e962-e968, 2010. https://doi.org/doi:10.4317/medoral.15.e962
  6. Thind BS, Stirrups DR, Lloyd CH: A comparison of tungsten-quartz-halogen, plasma arc and light-emitting diode light sources for the polymerization of an orthodontic adhesive. Eur J Orthod 28: 78-82, 2006. https://doi.org/10.1093/ejo/cji076
  7. Wataha JC, Lockwood PE, Lewis JB, Rueggeberg FA, Messer RL: Biological effects of blue light from dental curing units. Dent Mater 20: 150-157, 2004. https://doi.org/10.1016/S0109-5641(03)00086-1
  8. Lockwood DB, Wataha JC, Lewis JB, Tseng WY, Messer RL, Hsu SD: Blue light generates reactive oxygen species (ROS) differentially in tumor vs. normal epithelial cells. Dent Mater 21: 683-688, 2005. https://doi.org/10.1016/j.dental.2004.07.022
  9. Jeong MJ, Jeong SJ: Biological effects of light-emitting diodes curing unit on MDPC-23 cells and lipopolysaccharide stimulated MDPC-23 cells. J Dent Hyg Sci 19: 39-47, 2019. https://doi.org/10.17135/jdhs.2019.19.1.39
  10. Klapproth JM, Sasaki M: Bacterial induction of proinflammatory cytokines in inflammatory bowel disease. Inflamm Bowel Dis 16: 2173-2179, 2010. https://doi.org/10.1002/ibd.21332
  11. Striz I, Brabcova E, Kolesar L, Sekerkova A: Cytokine networking of innate immunity cells: a potential target of therapy. Clin Sci (Lond) 126: 593-612, 2014. https://doi.org/10.1042/CS20130497
  12. Moncada S, Higgs A: The L-arginine-nitric oxide pathway. N Engl J Med 329: 2002-2012, 1993. https://doi.org/10.1056/NEJM199312303292706
  13. Slots J, Gibbons RJ: Attachment of Bacteroides melaninogenicus subsp. asaccharolyticus to oral surfaces and its possible role in colonization of the mouth and of periodontal pockets. Infect Immun 19: 254-264, 1978. https://doi.org/10.1128/IAI.19.1.254-264.1978
  14. Birkedal-Hansen H, Taylor RE, Zambon JJ, Barwa PK, Neiders ME: Characterization of collagenolytic activity from strains of Bacteroides gingivalis. J Periodontal Res 23: 258-264, 1988. https://doi.org/10.1111/j.1600-0765.1988.tb01369.x
  15. Lee HO, Park JY: Antibacterial effect and cytotoxicity of desensitizer containing antimicrobial. J Dent Hyg Sci 15: 238-245, 2015. https://doi.org/10.17135/jdhs.2015.15.2.238
  16. Jeong SJ, Choi BD, Lee HY, et al.: 660 nm red LED induces secretory leukocyte protease inhibitor (SLPI) in lipopolysaccharide- stimulated RAW264.7 cell. J Nanosci Nanotechnol 15: 5610-5616, 2015. https://doi.org/10.1166/jnn.2015.10465
  17. Yalcin M, Kenar H, Dayi B, Sisman R, Karaoz E: The effect of light curing units on proliferation and senescence of human dental pulp mesenchymal stem cells. Int J Dent Sci Res 4: 10-16, 2016.
  18. Liebmann J, Born M, Kolb-Bachofen V: Blue-light irradiation regulates proliferation and differentiation in human skin cells. J Invest Dermatol 130: 259-269, 2010. https://doi.org/10.1038/jid.2009.194
  19. Liebmann J, Born M, Kolb-Bachofen V: Blue-light irradiation regulates proliferation and differentiation in human skin cells. J Invest Dermatol 130: 259-269, 2010. https://doi.org/10.1038/jid.2009.194
  20. Gorgidze LA, Oshemkova SA, Vorobjev IA: Blue light inhibits mitosis in tissue culture cells. Biosci Rep 18: 215-224, 1998. https://doi.org/10.1023/A:1020104914726
  21. Geminiani A, Caton JG, Romanos GE: Temperature change during non-contact diode laser irradiation of implant surfaces. Lasers Med Sci 27: 339-342, 2012. https://doi.org/10.1007/s10103-010-0876-8
  22. Cho KW, Lee SY, Chang BS, Um HS, Lee JK: The effect of photodynamic therapy on Aggregatibacter actinomycetemcomitans attached to surface-modified titanium. J Periodontal Implant Sci 45: 38-45, 2015. https://doi.org/10.5051/jpis.2015.45.2.38
  23. Bjordal JM, Couppe C, Chow RT, Tuner J, Ljunggren EA: A systematic review of low level laser therapy with location-specific doses for pain from chronic joint disorders. Aust J Physiother 49: 107-116, 2003. https://doi.org/10.1016/S0004-9514(14)60127-6