Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Non-hemolytic, Mucinous, Coagulase Negative MRSA Isolated from Urine

소변에서 분리된 비용혈성, 점액성, 응고효소 음성 MRSA

  • Kim, Jae Soo (Department of Laboratory Medicine, Dankook University Hospital) ;
  • Choi, Qute (Department of Laboratory Medicine, Chungnam National University College of Medicine) ;
  • Jung, Bo Kyeung (Department of Laboratory Medicine, Dankook University College of Medicine) ;
  • Kim, Jong Wan (Department of Laboratory Medicine, Dankook University College of Medicine) ;
  • Kim, Ga Yeon (Department of Public Health, Dankook University Graduate School)
  • 김재수 (단국대학교병원 진단검사의학과) ;
  • 최규태 (충남대학교 의과대학 진단검사의학과) ;
  • 정보경 (단국대학교 의과대학 진단검사의학과) ;
  • 김종완 (단국대학교 의과대학 진단검사의학과) ;
  • 김가연 (단국대학교 대학원 보건학과)
  • Received : 2019.03.30
  • Accepted : 2019.06.01
  • Published : 2019.06.30
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Abstract

An 84-year-old woman presented to the emergency department with a chief complaint of pressure sores of the anus. She had a urine catheter when she showed pyuria three times but had no fever. A microscopic examination revealed many grapevine-like Gram positive strains and neutrophils. After 24 hours of urine culture on blood agar, non-hemolytic mucous colonies were found and further enlarged after 48 hours of culture. The capsules were identified after India ink stain. The catalase was positive, but the tube coagulase and latex coagulase were both negative. The S. aureus was identified by Vitek-2 and mass spectrometer Vitek MS V-3 IVD. The strain was confirmed by 16S rRNA gene sequencing and multilocus sequence typing (MLST). The phenotypically atypical MRSA found in the tube coagulase and latex coagulase were both negative. MRSA often show no beta hemolysis as in this case but are rarely latex coagulase-negative. We report a woman whose urine culture showed non-hemolytic, tube coagulase-negative, and latex coagulase-negative MRSA.

84세 여성이 항문까지 욕창이 번져 응급실에 내원하였다. 그녀는 근처의 요양기관에서 전원되었고, 입원시 소변 도뇨관을 달고 있었으며 열은 없었다. 그람염색에서 많은 수의 포도상구균과 >25의 호중구 (${\times}1000$배 렌즈)가 관찰되었다. 24시간 배양한 혈액한천배지에서 용혈이 없는 점액성의 집락이 관찰되었으며 배양 48시간에는 점액성이 더욱 확대되었다. India ink로 염색하여 협막의 모습을 관찰하였으며, 카타라제 양성, 라텍스법 혈액응고효소 음성, 시험관법 혈액응고효소 음성소견을 보였다. Vitek-2와 질량분석기인 Vitek MS V-3 IVD에서 S. aureus로 나타났으며, 16S rRNA 유전자 염기서열 분석 및 Multilocus sequencing typing (MLST)에서 S. aureus로 표준균주인 ATCC 29213과 99.9% 일치를 보였다. 본 증례는 용혈성이 없고, 점액성이 강하며, 라텍스법 혈액응고효소 음성, 시험관법 혈액응고효소 음성소견을 보이는 MRSA를 동정하여 이를 보고한다.

Keywords

References

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