Asian-Australasian Journal of Animal Sciences

  • 제32권8호
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  • Pages.1172-1185
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  • 2019
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Metabolomic approach to key metabolites characterizing postmortem aged loin muscle of Japanese Black (Wagyu) cattle

  • Muroya, Susumu (Muscle Biology Research Unit, Animal Products Research Division, NARO Institute of Livestock and Grassland Science) ;
  • Oe, Mika (Muscle Biology Research Unit, Animal Products Research Division, NARO Institute of Livestock and Grassland Science) ;
  • Ojima, Koichi (Muscle Biology Research Unit, Animal Products Research Division, NARO Institute of Livestock and Grassland Science) ;
  • Watanabe, Akira (NARO Tohoku Agricultural Research Center)
  • 투고 : 2018.08.31
  • 심사 : 2018.12.20
  • 발행 : 2019.08.01
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초록

Objective: Meat quality attributes in postmortem muscle tissues depend on skeletal muscle metabolites. The objective of this study was to determine the key metabolic compounds and pathways that are associated with postmortem aging and beef quality in Japanese Black cattle (JB; a Japanese Wagyu breed with highly marbled beef). Methods: Lean portions of Longissimus thoracis (LT: loin) muscle in 3 JB steers were collected at 0, 1, and 14 days after slaughter. The metabolomic profiles of the samples were analyzed by capillary electrophoresis time-of-flight mass spectrometry, followed by statistical and multivariate analyses with bioinformatics resources. Results: Among the total 171 annotated compounds, the contents of gluconic acid, gluconolactone, spermidine, and the nutritionally vital substances (choline, thiamine, and nicotinamide) were elevated through the course of postmortem aging. The contents of glycolytic compounds increased along with the generation of lactic acid as the beef aging progressed. Moreover, the contents of several dipeptides and 16 amino acids, including glutamate and aromatic and branched-chain amino acids, were elevated over time, suggesting postmortem protein degradation in the muscle. Adenosine triphosphate degradation also progressed, resulting in the generation of inosine, xanthine, and hypoxanthine via the temporal increase in inosine 5'-monophosphate. Cysteine-glutathione disulfide, thiamine, and choline increased over time during the postmortem muscle aging. In the Kyoto encyclopedia of genes and genomes database, a bioinformatics resource, the postmortem metabolomic changes in LT muscle were characterized as pathways mainly related to protein digestion, glycolysis, citric acid cycle, pyruvate metabolism, pentose phosphate metabolism, nicotinamide metabolism, glycerophospholipid metabolism, purine metabolism, and glutathione metabolism. Conclusion: The compounds accumulating in aged beef were shown to be nutritionally vital substances and flavor components, as well as potential useful biomarkers of aging. The present metabolomic data during postmortem aging contribute to further understanding of the beef quality of JB and other breeds.

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참고문헌

  1. Feidt C, Petit A, Bruas-Reignier F, Brun-Bellut J. Release of free amino-acids during ageing in bovine meat. Meat Sci 1996;44:19-25. https://doi.org/10.1016/S0309-1740(96)00088-5
  2. Koutsidis G, Elmore JS, Oruna-Concha MJ, Campo MM, Wood JD, Mottram DS. Water-soluble precursors of beef flavour. Part II: Effect of post-mortem conditioning. Meat Sci 2008;79:270-7. https://doi.org/10.1016/j.meatsci.2007.09.010
  3. Farmer LJ, Mottram DS, Whitfield FB. Volatile compounds produced in Maillard reactions involving cysteine, ribose and phospholipid. J Sci Food Agric 1989;49:347-68. https://doi.org/10.1002/jsfa.2740490311
  4. Watanabe A, Tsuneishi E, Takimoto Y. Analysis of ATP and its breakdown products in beef by reversed-phase HPLC. J Food Sci 1989;54:1169-72. https://doi.org/10.1111/j.1365-2621.1989.tb05948.x
  5. Huff-Lonergan E, Zhang W, Lonergan SM. Biochemistry of postmortem muscle - lessons on mechanisms of meat tenderization. Meat Sci 2010;86:184-95. https://doi.org/10.1016/j.meatsci.2010.05.004
  6. Jiang T, Bratcher CL. Differentiation of commercial ground beef products and correlation between metabolites and sensory attributes: a metabolomic approach. Food Res Int 2016;90:298-306. https://doi.org/10.1016/j.foodres.2016.11.002
  7. Lee SM, Kwon GY, Kim KO, Kim YS. Metabolomic approach for determination of key volatile compounds related to beef flavor in glutathione-Maillard reaction products. Anal Chim Acta 2011;703:204-11. https://doi.org/10.1016/j.aca.2011.07.028
  8. Carrillo JA, He Y, Li Y, et al. Integrated metabolomic and transcriptome analyses reveal finishing forage affects metabolic pathways related to beef quality and animal welfare. Sci Rep 2016;6:25948. https://doi.org/10.1038/srep25948
  9. Argyri AA, Mallouchos A, Panagou EZ, Nychas GJ. The dynamics of the HS/SPME-GC/MS as a tool to assess the spoilage of minced beef stored under different packaging and temperature conditions. Int J Food Microbiol 2015;193:51-8. https://doi.org/10.1016/j.ijfoodmicro.2014.09.020
  10. Ma D, Kim YHB, Cooper B, et al. Metabolomics profiling to determine the effect of postmortem aging on color and lipid oxidative stabilities of different bovine muscles. J Agric Food Chem 2017;65:6708-16. https://doi.org/10.1021/acs.jafc.7b02175
  11. Kodani Y, Miyakawa T, Komatsu T, Tanokura M. NMR-based metabolomics for simultaneously evaluating multiple determinants of primary beef quality in Japanese Black cattle. Sci Rep 2017;7:1297. https://doi.org/10.1038/s41598-017-01272-8
  12. Muroya S, Oe M, Nakajima I, Ojima K, Chikuni K. CE-TOF MS-based metabolomic profiling revealed characteristic metabolic pathways in postmortem porcine fast and slow type muscles. Meat Sci 2014;98:726-35. https://doi.org/10.1016/j.meatsci.2014.07.018
  13. Imanari M, Higuchi M, Shiba N, Watanabe A. Accurate analysis of taurine, anserine, carnosine and free amino acids in a cattle muscle biopsy sample. Anim Sci J 2010;81:369-76. https://doi.org/10.1111/j.1740-0929.2010.00751.x
  14. Kitamura S, Muroya S, Tanabe S, Okumura T, Chikuni K, Nishimura T. Mechanism of production of troponin T fragments during postmortem aging of porcine muscle. J Agric Food Chem 2005;53:4178-81. https://doi.org/10.1021/jf047974l
  15. Muroya S, Kitamura S, Tanabe S, Nishimura T, Nakajima I, Chikuni K. N-terminal amino acid sequences of troponin T fragments, including 30 kDa one, produced during postmortem aging of bovine longissimus muscle. Meat Sci 2004;67:19-24. https://doi.org/10.1016/j.meatsci.2003.08.018
  16. Muroya S, Nakajima I, Oe M, Chikuni K. Difference in postmortem degradation pattern among troponin T isoforms expressed in bovine longissimus, diaphragm, and masseter muscles. Meat Sci 2006;72:245-51. https://doi.org/10.1016/j.meatsci.2005.07.008
  17. Muroya S, Ohnishi-Kameyama M, Oe M, Nakajima I, Chikuni K. Postmortem changes in bovine troponin T isoforms on two-dimensional electrophoretic gel analyzed using mass spectrometry and western blotting: The limited fragmentation into basic polypeptides. Meat Sci 2007;75:506-14. https://doi.org/10.1016/j.meatsci.2006.08.012
  18. Muroya S, Nakajima I, Chikuni K. Amino acid sequences of multiple fast and slow troponin T isoforms expressed in adult bovine skeletal muscles. J Anim Sci 2003;81:1185-92. https://doi.org/10.2527/2003.8151185x
  19. Nishimura T. Mechanism involved in the improvement of meat taste during postmortem aging. Food Sci Technol Int Tokyo 1998;4:241-9. https://doi.org/10.3136/fsti9596t9798.4.241
  20. Voet D, Voet JG. Biochemistry. 2nd ed. ed. New York, USA: John Wiley & Sons, Inc.; 1995.
  21. Penny IF, Dransfield E. Relationship between toughness and troponin T in conditioned beef. Meat Sci 1979;3:135-41. https://doi.org/10.1016/0309-1740(79)90015-9
  22. Jung DW, Hong JH, Kim KO. Sensory characteristics and consumer acceptability of beef soup with added glutathione and/or MSG. J Food Sci 2010;75:S36-S42. https://doi.org/10.1111/j.1750-3841.2009.01411.x
  23. Maehashi K, Matsuzaki M, Yamamoto Y, Udaka S. Isolation of peptides from an enzymatic hydrolysate of food proteins and characterization of their taste properties. Biosci Biotechnol Biochem 1999;63:555-9. https://doi.org/10.1271/bbb.63.555
  24. Zeisel SH. Choline: critical role during fetal development and dietary requirements in adults. Annu Rev Nutr 2006;26:229-50. https://doi.org/10.1146/annurev.nutr.26.061505.111156
  25. Terasaki M, Kajikawa M, Fujita E, Ishii K. Studies on the flavor of meats, Part I. Formation and degradation of inosinic acids in meats. Agric Biol Chem 1965;29:208-15. https://doi.org/10.1080/00021369.1965.10858377
  26. Tikk M, Tikk K, Torngren MA, et al. Development of inosine monophosphate and its degradation products during aging of pork of different qualities in relation to basic taste and retronasal flavor perception of the meat. J Agric Food Chem 2006;54:7769-77. https://doi.org/10.1021/jf060145a
  27. Asano T, Yuasa K, Kunugita K, Teraji T, Mitsuoka T. Effects of gluconic acid on human faecal bacteria. Microb Ecol Health Dis 1994;7:247-56. https://doi.org/10.3109/08910609409141362
  28. Kibe R, Kurihara S, Sakai Y, et al. Upregulation of colonic luminal polyamines produced by intestinal microbiota delays senescence in mice. Sci Rep 2014;4:4548. https://doi.org/10.1038/srep04548
  29. Poel C, Backermann S, Ternes W. Degradation and conversion of thiamin and thiamin phosphate esters in fresh stored pork and in raw sausages. Meat Sci 2009;83:506-10. https://doi.org/10.1016/j.meatsci.2009.06.034
  30. Muroya S, Oe M, Ojima K. Thiamine accumulation and thiamine triphosphate decline occur in parallel with ATP exhaustion during postmortem aging of pork muscles. Meat Sci 2018;137:228-34. https://doi.org/10.1016/j.meatsci.2017.11.035
  31. Manzetti S, Zhang J, van der Spoel D. Thiamin function, metabolism, uptake, and transport. Biochemistry 2014;53:821-35. https://doi.org/10.1021/bi401618y

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