The Journal of the Convergence on Culture Technology (문화기술의 융합)

The International Promotion Agency of Culture Technology (국제문화기술진흥원)
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The Effects of Protecting Liver and Improving Liver Function on Cabbage Extract

양배추 추출물의 간보호 및 간기능 개선 효과

  • 김현경 (서원대학교 식품공학과)
  • Received : 2019.02.13
  • Accepted : 2019.04.17
  • Published : 2019.05.31
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Abstract

This study investigated the pharmaceutical extraction and the functional health food extraction, which have a beneficial effect on the human body and which can be used safely for a long period of time without adverse side effects and also have excellent effects of protecting liver and improving liver function. As the results, the cabbage extract does not show cytotoxicity, and thus can be used safely. In an experiment performed on an animal model with liver injury induced by a drug (APAP), it could be seen that the cabbage extract exhibited the effects of protecting liver and improving liver function by effectively reducing AST and ALT which are liver injury markers, indicating that the cabbage extract is effective as a pharmaceutical extraction for preventing or treating liver disease. In particular, the cabbage extract was effective in treating inflammation of the liver by reducing the expression of the inflammatory mediators iNOS and COX-2 and the proinflammatory cytokine $IL-1{\beta}$, which are involved in acute inflammatory reactions accompanying liver injury. In the results, an extract of cabbage heat-treated at a temperature of 100 to $150^{\circ}C$ had a better liver function-improving effect or anti-inflammatory effect than an extract of raw cabbage.

본 연구는 인체에 유익한 효과가 있고 부작용 없이 장기간 안전하게 사용할 수 있으며 간보호 및 간기능 개선에 탁월한 효과가 있는지 양배추출물의 간보호 효과에 대하여 알아보았다. 약물(APAP)에 의해 유도된 간손상 동물모델에서 수행된 실험에서, 양배추 추출물은 간손상 마커인 AST 및 ALT를 효과적으로 감소시킴으로써 간 보호 및 간 기능 개선 효과를 나타냄을 알 수 있었다. 양배추 추출물은 간질환의 예방 또는 치료용 약학적 추출물로서 유효하다는 것이다. 특히, 양배추 추출물은 간손상에 수반되는 급성 염증 반응에 관여하는 염증 매개체 iNOS 및 COX-2 및 전염증성 사이토카인 $IL-1{\beta}$의 발현을 감소시킴으로써 간염 염증을 치료하는데 효과적이었다. 그 결과, $100{\sim}150^{\circ}C$에서 열처리 한 배추 추출물은 생배추 추출물에 비해 간기능 개선 효과 또는 항염증 효과가 우수 하였다.

Keywords

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표 1. RT_PCR에 의한 사이토카인 발현 측정 Table 1. Measurement of cytokine expression by RT-PCR

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그림 1. 양배추 추출물의 세포독성 (cell Viability) 평가 Figure 1. Cell viability assay in RAW 264.7 cells treated with cabbage extracts.

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그림 2. 마우스의 혈청에서 (A) Superoxide Dismutase (SOD), (B) 글루타티온 퍼 옥시 다제 (GPx) 및 (C) 카탈라아제 활성 (CAT) Figure 2. (A) Superoxide dismutase (SOD), (B) glutathione peroxidase (GPx) and (C) catalase activity (CAT) in the serum of mice. € indicates p < 0.05 as compared to the control group, *** indicates p < 0.001 compared to acetaminophen, and # indicates p < 0.01 as compared to the positive control, NAC.

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그림 3. 마우스의 혈청에서 8-Oxo-2'-dexoxyguanosine (8-OHdG) 수준 감소 효과 Figure 3. 8-Oxo-2’-dexoxyguanosine (8-OHdG) levels in the serum of mice. $ indicates p < 0.05 as compared to the control group, *** indicates p < 0.001 compared to acetaminophen, and # indicates p < 0.01 as compared to the positive control, NAC.

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그림 4. 혈청에서 (A) Alanine aminotransferase (ALT) and (B) aspartate transaminase (AST) 레벨 측정 Figure 4. (A) Alanine aminotransferase (ALT) and (B) aspartate transaminase (AST) levels in the serum. # indicates p < 0.05 as compared to the control group, *** indicates p < 0.001 compared to acetaminophen, and # indicates p < 0.01 as compared to the positive control, NAC.

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그림 5. 양배추 추출물의 항염증효과 및 간기능 개선 효과 Figure 5. Anti-inflammatory effect and hepatoprotective effect on cabbage extracts.

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그림 6. 간 조직의 Hematoxylin & Eosin 염색 Figure 6.. Hematoxylin & Eosin (H&E) staining of sections of the liver of mice in group (A) control, (B) acetaminophen, (C) NAC, (D) cabbage. Images were taken at a magnification of 400x under a microscope.

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