Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization and Genomic Analysis of Novel Bacteriophage ΦCS01 Targeting Cronobacter sakazakii

  • Kim, Gyeong-Hwuii (Department of Biological Science and Technology, Yonsei University) ;
  • Kim, Jaegon (Department of Biological Science and Technology, Yonsei University) ;
  • Kim, Ki-Hwan (Department of Biological Science and Technology, Yonsei University) ;
  • Lee, Jin-Sun (Department of Biological Science and Technology, Yonsei University) ;
  • Lee, Na-Gyeong (Department of Biological Science and Technology, Yonsei University) ;
  • Lim, Tae-Hyun (Department of Biological Science and Technology, Yonsei University) ;
  • Yoon, Sung-Sik (Department of Biological Science and Technology, Yonsei University)
  • Received : 2018.12.26
  • Accepted : 2019.04.09
  • Published : 2019.05.28
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Abstract

Cronobacter sakazakii is an opportunistic pathogen causing serious infections in neonates. In this study, a bacteriophage ${\Phi}CS01$, which infects C. sakazakii, was isolated from swine feces and its morphology, growth parameters, and genomic analysis were investigated. Transmission electron microscopy revealed that ${\Phi}CS01$ has a spherical head and is 65.74 nm in diameter with a 98.75 nm contracted tail, suggesting that it belongs to the family Myoviridae. The major viral proteins are approximately 71 kDa and 64 kDa in size. The latent period of ${\Phi}CS01$ was shown to be 60 min, and the burst size was 90.7 pfu (plaque-forming units)/infected cell. Bacteriophage ${\Phi}CS01$ was stable at $4-60^{\circ}C$ for 1 h and lost infectivity after 1 h of heating at $70^{\circ}C$. Infectivity remained unaffected at pH 4-9 for 2 h, while the bacteriophage was inactivated at pH <3 or >10. The double-stranded ${\Phi}CS01$ DNA genome consists of 48,195 base pairs, with 75 predicted open reading frames. Phylogenetic analysis is closely related to that of the previously reported C. sakazakii phage ESP2949-1. The newly isolated ${\Phi}CS01$ shows infectivity in the host bacterium C. sakazakii, indicating that it may be a promising alternative to antibacterial agents for the removal of C. sakazakii from powdered infant formulas.

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References

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