Journal of Naturopathy (한국자연치유학회지)

Koean Soceity for Naturopathy (한국자연치유학회)
권호 표지
DOI QR코드

DOI QR Code

A Study on Far-infrared Radiation and Proliferation of Ocherous Cotton Quilt Fabrics

황토를 부착한 이불 면 원단의 원적외선 방출량 및 생균의 분리 동정 연구

  • Lee, Ku Yeon (Graduate School of Integrated Medicine, Cha University) ;
  • Lee, Hyung H. (Department of Biological Sciences, Konkuk Unviersity) ;
  • Hahm, Suk Chan (Graduate School of Integrated Medicine, Cha University)
  • 이구연 (차의과학대학교 통합의학대학원) ;
  • 이형환 (건국대학교 생명과학과) ;
  • 함석찬 (차의과학대학교 통합의학대학원)
  • Received : 2019.09.12
  • Accepted : 2019.09.16
  • Published : 2019.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: The purpose of this study was to investigate the far-infrared emissivity of patented ocher quilt cotton fabrics and to investigate the microorganisms that survived the washing of cotton fabrics up to 20 times. Methods: A 16S rRNA assay was performed using a far-infrared radiometer and a single colony in which microorganisms grew in nutrient media. Results: The far-infrared emissivity of ocher quilt was 0.902 (90.2%) at 5~20 ㎛ at 40℃, and the radiation energy was 3.63 × 102 w/m2. The number of viable cells was 2.0 × 102 cells/ml in ocher duvet cotton fabric, and no viable bacteria found in regular cotton fabric. The base sequence of 16S rRNA of B-2 strain isolated into single colonies was 1,419 bases, and the base sequence of strain A-4 was 1,284 bases. The base sequence of 16S rRNA of these two strains showed high homology with Bacillus spp. The B-2 bacteria showed high homology with 99.0% of the 16S rRNA sequence of B. aryabhattai EF114313 and 99.0% of the A-4 bacteria of B. bingmayongensis AKCS01000011. Consequently the colony strain B-2 finally identified as B. aryabhattai BJ-2 and A-4 as B. bingmayongensis BJ-4 strain. Concusions: Soil Bacillus strains survived in ocher quilt cotton fabric after 20 washing. The material can be useful because quilt cotton fabric emits a large amount of far-infrared and far-infrared radiation energy.

목적: 특허 받은 황토 면 원단의 원적외선 방사율 및 면 원단을 20회까지 세탁을 하였을 때에 어떠한 미생물이 존재하는지를 조사하여 분리 동정하는 것이 목적이었다. 방법: 원적외선 방사능 측정기와 미생물을 영양배지에 배양하여 증식하는 단일 콜로니를 이용하여 16S rRNA법으로 동정하였다. 결과: 황토이불 면의 원적외선의 방사율은 40℃에서 5~20 ㎛에서 0.902(90.2%)이었고, 방사에너지는 3.63 × 102 w/m2로 높게 나타났다. 황토 이불 면 원단에서 2.0 × 102 cells/ml의 생균수가 존재하였고, 그리고 일반 면 원단에서는 생균이 발견되지 않았다. 단일 콜로니로 분리된 B-2 균주의 16S rRNA의 염기서열은 1,419 bases이었고, A-4균주의 염기서열은 1,284 bases이었다. 이 두 균주의 16S rRNA의 염기서열은 Bacillus 속 세균들과 높은 상동성을 보이었다. B-2 균주는 B. aryabhattai EF114313의 16S rRNA 염기서열과 99.0% 그리고 A-4균주는 B. bingmayongensis AKCS01000011의 16S rRNA 염기서열과 99.0%의 높은 상동성이 나타났다. 상기의 결과들을 활용하여 16S rRNA을 이용하여 유연관계를 조사하여 콜로니 균주 B-2를 B. aryabhattai BJ-2 균주로, A-4를 B. bingmayongensis BJ-4 균주로 최종 동정하였다. 결론: 황토 이불 면 원단에서 두 종류의 간균을 발견하였고, 이불 면 원단이 높은 량의 원적외선 및 원적외선 방사에너지가 발산하므로 유용하게 이용될 수 있다고 본다.

Keywords

References

  1. Adetunji, A.I. and A.O. Olaniran. 2018. Immobilization and characterization of lipase from an indigenous Bacillus aryabhattai SE3-PB isolated from lipid-rich wastewater. Prep. Biochem. Biotechnol. 48(10):898-905.
  2. Balakrishna, P.A., K.A. Jaya, K. Thulasi and H. Kumarapillai. 2017. Evaluation of short-chain-length polyhydroxyalkanoate accumulation in Bacillus aryabhattai. Braz. J. Microbiol. 48(3):451-460.
  3. Gregory, T.R. 2008. Understanding evolutionary trees. Evolution: Educ. Outreach 1(2): 121-137.
  4. Kim, J.H., Y.T. Lim, H.S. Jang, and S.Y. So. 2018. A study on the comparison of far-infrared rays emission of regional Hwangto. Kor. Conc. Inst. Proc. 30(1): 663-664.
  5. Lee, G.S. 2003. Study on the influence of the Hwangtoh on the growth and plants. Mokpo National University, Korea, Thesis of Master's Sciences, p. 1.
  6. Liu, B, G.H. Liu, G.P. Hu, C. Sengonca, N.Q. Lin, J.Y. Tang, W.Q. Tang, and Y.Z. Lin. 2014. Bacillus bingmayongensis sp. nov., isolated from the pit soil of Emperor Qin's Terra-cotta warriors in China. Antonie Van Leeuwenhoek. 105(3):501-510.
  7. Oh, S.W. 2016. Electrical Properties and Far-infrared Ray Emission of Ceramics Manufactured with Sawdust and Rice Husk. J. Kor. Wood Sci. Techn. 44(1):106-112.
  8. Park, M.G. 1997. Discovered the far-infrared efficacy of ocher - Ocher saves dying life. Saemter 28(11): 10-14. Saemter Pub. Co., Seoul.
  9. Park, S.O., J.H. Oh, J.K. Kim, and J.O. Moon. 2015. A study of loess·charcoal board panel development and application plan for atopy improvement. J. Resident. Environ. Inst. Kor. 13(2):135-143.
  10. Ryu, D.O. 1997. Ocher mystery. Haenglim Pub. Co., Seoul, p.20.
  11. Paz, A, D. Outeirino, R. Pinheiro de Souza Oliveira, and J.M. Dominguez. 2018. Fed-batch production of vanillin by Bacillus aryabhattai BA03. N Biotechnol. 40(PtB): 186-191.
  12. Pal, R.M., S. Datta, and S. Ghosh. 2017. A novel extracellular low-temperature active phytase from Bacillus aryabhattai RS1 with potential application in plant growth. Biotechnol Prog. 33(3): 633-641. doi: 10.1002/btpr.2452.
  13. Rinttila, T., A. Kassinen, F. Malinene, I. Krogius, and A. Palva. 2004. Development of an extensive a set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in fecal samples by real-time PCR. J. Appl. Microbiol. 97(5):1166-1177.
  14. Ryu, S.W. and H.H. Lee. 2018. Distribution of beneficial bacteria in the intestine after Ezamin ingestion of Bacillus subtilis AK strain fermentation. J. Naturopathy 7(2):27-28.
  15. Ryu, S.W. and H.H. Lee. 2019. Effects of the proliferation of beneficial and harmful enteric bacteria after intake of soybean fermentation(Zen) produced by a mixture of Lactobacillus and Saccharomyces. J. Naturopathy 8(1): 1-19.
  16. Schmittgen, T.D. and K.J. Livak. 2008. Analyzing real-time PCR data by the comparative C(T)method. Nat. Protoc. 3(5):1101-1108.