Parasites, Hosts and Diseases

  • 제57권2호
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  • Pages.197-200
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  • 2019
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Molecular Prevalence of Cryptosporidium spp. in Breeding Kennel Dogs

  • Itoh, Naoyuki (Laboratory of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University) ;
  • Tanaka, Hazuki (Laboratory of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University) ;
  • Iijima, Yuko (Laboratory of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University) ;
  • Kameshima, Satoshi (Laboratory of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University) ;
  • Kimura, Yuya (Laboratory of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University)
  • 투고 : 2018.12.26
  • 심사 : 2019.02.27
  • 발행 : 2019.04.30
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초록

Cryptosporidium is a common intestinal protozoan that can lead to diarrhea in humans and dogs. The predominant species of infection are C. hominis and C. parvum in humans, and C. canis in dogs. However, C. canis can infect immunocompromised humans. Considering the close contact with humans, dogs have the potential to be reservoirs for human cryptosporidiosis. Breeding kennels are the major supply source of puppies for pet shops. The present study is to determine the molecular prevalence and characteristics of Cryptosporidium spp. found in breeding kennel dogs. A total of 314 fecal samples were collected from young and adult dogs kept in 5 breeding kennels. A polymerase chain reaction targeting the small subunit rRNA gene was employed for the detection of Cryptosporidium spp. To determine the species, the DNA sequences were compared to GenBank data. Overall, 21.0% of the fecal samples were positive for Cryptosporidium spp. infection. Cryptosporidium spp. was detected in all 5 facilities. A sequencing analysis demonstrated that all isolates shared 99-100% similarity with C. canis. The results suggest that Cryptosporidium spp. infection is present at a high-level in breeding kennel dogs. However, because dominant species in this survey was C. canis, the importance of breeding kennel dogs as reservoirs for Cryptosporidium spp. transmission to humans is likely to be low in Japan.

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참고문헌

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피인용 문헌

  1. Occurrence and genotypes of Cryptosporidium spp., Giardia duodenalis , and Blastocystis sp. in household, shelter, breeding, and pet market dogs in Guangzhou, southern China vol.10, pp.1, 2019, https://doi.org/10.1038/s41598-020-74299-z