Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Establishment of an efficient regeneration system for Hibiscus syriacus 'Nanpa'

무궁화 품종 '난파'의 효율적인 재분화 체계 확립

  • Son, Ji-Hi (Department of Biotechnology, Jeju National University) ;
  • Sun, Hyeon-Jin (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Kang, Hong-Gyu (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Suh, Seok-Chul (Department of Molecular Biotechnology, College of Applied Life Sciences, Jeju National University) ;
  • Lee, Hyo-Yeon (Department of Molecular Biotechnology, College of Applied Life Sciences, Jeju National University)
  • 손지희 (제주대학교 생명공학과) ;
  • 선현진 (제주대학교 아열대원예산업연구소) ;
  • 강홍규 (제주대학교 아열대원예산업연구소) ;
  • 서석철 (제주대학교 생명공학부) ;
  • 이효연 (제주대학교 생명공학부)
  • Received : 2019.12.02
  • Accepted : 2019.12.20
  • Published : 2019.12.31
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Abstract

Hibiscus syriacus L., the national flower of Korea, is a commonly grown ornamental species found in parks, gardens and along roadsides in Korea. This study sought to establish a plant regeneration system of H. syriacus 'Nanpa' using mature leaves as an explant. Sterilized mature leaf explants were cultured on modified MS medium with combinations of NAA and BAP at various concentrations for 6 weeks. Among the various media evaluated, modified MS media containing 0.46 mg/L NAA and 0.5 mg/L BAP (CI-1) or 0.92 mg/L NAA and 1 mg/L BAP (CI-4) were the most effective for callus formation. Whitish-yellow calluses were observed on CI-1 medium, while green calluses formed on CI-4 medium. The whitish-yellow callus derived from CI-1 medium showed a higher frequency of shoot induction than the green callus derived from CI-4 on modified MS medium containing 0.5 mg/L BAP. Among the various media evaluated in this study, modified MS medium containing 0.46 mg/L NAA and 0.5 mg/L BAP was the most optimal for shoot-forming callus production. Our findings show that mature leaves of H. syriacus 'Nanpa' can be used for in vitro plant regeneration, and the regeneration system described here may be a powerful tool for molecular breeding of H. syriacus 'Nanpa' in the future.

Keywords

References

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