한국양봉학회지 (Journal of Apiculture)

한국양봉학회 (The Apicultural Society of Korea)
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Anti-Parasitic Activity of Lespedeza cuneata Extract on Causative Agent of Nosemosis Type C, Nosema ceranae

  • Song, Hyunchan (Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim, Hyekyung (Department of Industrial Entomology, Korea National College of Agriculture and Fisheries) ;
  • Kim, Ki-Young (Graduate School of Biotechnology, Kyung Hee University)
  • 투고 : 2019.02.22
  • 심사 : 2019.05.03
  • 발행 : 2019.06.30
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초록

Although honeybees (Apis mellifera) are crucial for maintenance of the ecosystem, population of honeybee has been steadily decreasing due to diseases including nosemosis. Nosemosis is a disease caused by Nosema ceranae and is now considered as a major threat to honeybees. N. ceranae is a microsporidian that stays in form of spore even before the infection, which makes it harder to control than other pathogens. People are now aware of this parasite, however, cure and preventive candidates for nosemosis are hardly found until today. In this study, in vitro experiment of Lespedeza cuneata treatment to prevent nosemosis were done using Trichoplusia ni cell line, BTI-TN5B1-4. Normal T. ni cells exhibited round shape without abnormal size. On the other hand, when N. ceranae were treated, cells deteriorated and some cells abnormally enlarged due to N. ceranae infection. Interestingly, treatment of T. ni cells with L. cuneate extract protected abnormal cell shape induced by N. ceranae infection to normal shape. Some N. ceranae spores were observed outside of the cells. Effective concentration range for N. ceranae control were experimented. Lowest concentration which can control nosemosis were 50 ㎍/mL. When the concentration of L. cuneata extract was exceeded 200 ㎍/mL, cytotoxicity started to show up.

키워드

참고문헌

  1. Allred, B. W., S. D. Fuhlendorf, T. A. Monaco and R. E. Will. 2010. Morphological and physiological traits in the success of the invasive plant Lespedeza cuneata. Biol. Invasions. 12(4): 739-749. https://doi.org/10.1007/s10530-009-9476-6
  2. Ellis, J. D., J. D. Evans and J. Pettis. 2010. Colony losses, managed colony population decline, and Colony Collapse Disorder in the United States. J. Apic. Res. 49(1): 134-136. https://doi.org/10.3896/IBRA.1.49.1.30
  3. Evans, J. D., C. Saegerman, C. Mullin, E. Haubruge, B. K. Nguyen, M. Frazier, J. Frazier, D. Cox-Foster, Y. Chen, R. Underwood, D. R. Tarpy and J. S. Pettis. 2009. Colony collapse disorder: a descriptive study. PloS ONE. 4(8): e6481. https://doi.org/10.1371/journal.pone.0006481
  4. Fenoy, S., C. Rueda, M. Higes, R. Martin-Hernandez and C. Del Aguila. 2009. High-level resistance of Nosema ceranae, a parasite of the honeybee, to temperature and desiccation. Appl. Environ. Microbiol. 75(21): 6886-6889. https://doi.org/10.1128/AEM.01025-09
  5. Fries, I. 2010. Nosema ceranae in European honey bees (Apis mellifera). J. Invertebr. Pathol. 103: S73-S79. https://doi.org/10.1016/j.jip.2009.06.017
  6. Fries, I. 1988. Infectivity and multiplication of Nosema apis Z. in the ventriculus of the honey bee. Apidologie. 19(3): 319-328. https://doi.org/10.1051/apido:19880310
  7. Hges, M., R. Martin-Hernandez and A. Meana. 2010. Nosema ceranae in Europe: an emergent type C nosemosis. Apidologie. 41(3): 375-392. https://doi.org/10.1051/apido/2010019
  8. Huang, W. F., L. F. Solter, P. M. Yau and B. S. Imai. 2013. Nosema ceranae escapes fumagillin control in honey bees. PLoS Pathog. 9(3): e1003185. https://doi.org/10.1371/journal.ppat.1003185
  9. Kim, D. J., H. G. Yun, I. H. Kim, W. S. Gwak and S. D. Woo. 2017. Efficient Method for the Rapid Purification of Nosema ceranae Spores. Mycobiology. 45(3): 204-208. https://doi.org/10.5941/MYCO.2017.45.3.204
  10. Klee, J., A. M. Besana, E. Genersch, S. Gisder, A. Nanetti, D. Q. Tam, T. X. Chinh, F. Puerta, J. M. Ruz, P. Kryger, D. Message, F. Hatjina, S. Korpela, I. Fries and R. J. Paxton. 2007. Widespread dispersal of the microsporidian Nosema ceranae, an emergent pathogen of the western honey bee, Apis mellifera. J. Invertebr. Pathol. 96(1): 1-10. https://doi.org/10.1016/j.jip.2007.02.014
  11. Mayack, C. and D. Naug. 2009. Energetic stress in the honeybee Apis mellifera from Nosema ceranae infection. J. Invertebr. Pathol. 100(3): 185-188. https://doi.org/10.1016/j.jip.2008.12.001
  12. Morse, R. A. and N. W. Calderone. 2000. The value of honey bees as pollinators of US crops in 2000. Bee Culture 128(3): 1-15.