Journal of Apiculture (한국양봉학회지)

The Apicultural Society of Korea (한국양봉학회)
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In vitro Antifungal Activity of 4-Hydroxyderricin and Acetylshikonin against Ascosphaera apis

  • Park, Sangchul (Graduate School of Biotechnology, Kyung Hee University) ;
  • Shin, Yu-Kyong (College of Life Science, Kyung Hee University) ;
  • Cho, MyoungLae (National Development Institute of Korean Medicine) ;
  • Kwon, Hyun Sook (National Development Institute of Korean Medicine) ;
  • Kwon, Yun Ju (National Development Institute of Korean Medicine) ;
  • Kim, Ki-Young (Graduate School of Biotechnology, Kyung Hee University)
  • Received : 2019.01.16
  • Accepted : 2019.02.19
  • Published : 2019.06.30
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Abstract

Honey bees are important pollinators in agriculture, but are threatened by the pathogen Ascosphaera apis, which causes chalkbrood. Despite attempts to control this fungus using synthetic fungicides, none of them have been proven to be completely effective. Among 640 natural compounds that we tested, 4-hydroxyderricin (MIC=3.125, 6.25 mg/L after 24 h and 48 h growth, respectively) exhibited the strongest anti- Ascosphaera apis activity, followed by acetylshikonin (MIC=12.5 mg/L for 24 h and 48 h growth). 4-Hydroxyderricin showed selective growth inhibition of Ascosphaera apis and Rhizopus oryzae among tested fungus strains. Treatment 4-hydroxyderricin with miconazole revealed a synergistic effect (FICI=0.65±0.13 at 48 h incubation). These findings suggest that 4-hydroxyderricin, which has antifungal activity against Ascosphaera apis but few other fungal species, can effectively control infectious fungal diseases. Combined treatment of bees with 4-hydroxyderricin and miconazole could reduce cytotoxicity and improve the cost effectiveness of treatment.

Keywords

References

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