Journal of Microbiology and Biotechnology

  • 제29권4호
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  • Pages.571-576
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  • 2019
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Arctigenin Inhibits Etoposide Resistance in HT-29 Colon Cancer Cells during Microenvironmental Stress

  • Yoon, Sae-Bom (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University)
  • 투고 : 2019.01.28
  • 심사 : 2019.03.27
  • 발행 : 2019.04.28
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초록

Microenvironmental stress, which is naturally observed in solid tumors, has been implicated in anticancer drug resistance. This tumor-specific stress causes the degradation of topoisomerase $II{\alpha}$, rendering cells resistant to topoisomerase $II{\alpha}$-targeted anticancer agents. In addition, microenvironmental stress can induce the overexpression of 78kDa glucose regulated protein (GRP78), which can subsequently block the activation of apoptosis induced by treatment with anticancer agents. Therefore, inhibition of topoisomerase $II{\alpha}$ degradation and reduction in GRP78 expression may be effective strategies for inhibiting anticancer drug resistance. In this study, we investigated the active compound arctigenin, which inhibited microenvironmental stress-induced etoposide resistance in HT-29 cells. Arctigenin was also highly toxic to etoposide-resistant HT-29 cells, with an $IC_{50}$ value of $10{\mu}M$ for colony formation. We further showed that arctigenin inhibited the degradation of topoisomerase $II{\alpha}$ and reduced the expression of GRP78. Thus, these results suggest that arctigenin is a novel therapeutic agent that inhibits resistance to etoposide associated with microenvironmental stress conditions.

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참고문헌

  1. Goodman GE, Yen YP, Cox TC, Crowley J. 1987. Effect of verapamil on in vitro cytotoxicity of adriamycin and vinblastine in human tumor cells. Cancer Res. 47: 2295-2304.
  2. Ahn HJ, Kim JY, Lee CH, Song IS, Liu KH. 2008. Screening of chemosensitizer candidates using natural extracts. J. Life Sci. 9: 1244-1248.
  3. Shen DW, Goldenberg S, P astan I, Gottesman MM. 2000. Decreased accumulation of [$^{14}C$] carboplatin in human cisplatin-resistant cells results from reduced energy dependent uptake. J. Cell Physiol. 183: 108-116. https://doi.org/10.1002/(SICI)1097-4652(200004)183:1<108::AID-JCP13>3.0.CO;2-4
  4. Goto S, Kamada K, Soh Y, Ihara Y, Kondo T. 2002. Significance of nuclear glutathione S-transferase pi in resistance to anti-cancer drugs. Jpn. J. Cancer Res. 93: 1047-1056. https://doi.org/10.1111/j.1349-7006.2002.tb02482.x
  5. Axelson H, Fredlund E, Ovenberger M, Landberg G, Pahlman S. 2005. Hypoxia-induced dedifferentiation of tumor cells-A mechanism behind heterogeneity and aggressiveness of solid tumors. Semin. Cell DevBiol. 16: 554-563. https://doi.org/10.1016/j.semcdb.2005.03.007
  6. Brown JM, Giaccia AJ. 1998. The unique physiology of solid tumors: opportunities (and problems) for cancer therapy. Cancer Res. 58: 1408-1416.
  7. Tomida A, Tsuruo T. 1999. Drug resistance mediated by cellular stress response to the microenvironment of solid tumors. Anticancer Drug Des. 14: 169-177.
  8. Acker T, Plate KH. 2002. A role for hypoxia and hypoxia-inducible transcription factors in tumor physiology. J. Mol. Med. 80: 562-575. https://doi.org/10.1007/s00109-002-0355-1
  9. Vaupel P, Yhews O, Hoeckel M. 2001. Treatment resistance of solid tumors: role of hypoxia and anemia. Med. Oncol. 18: 243-259. https://doi.org/10.1385/MO:18:4:243
  10. Chen AY, Liu LF. 1994. Mechanisms of resistance to topoisomerase inhibitors. Cancer Treat. Res. 73: 263-281. https://doi.org/10.1007/978-1-4615-2632-2_13
  11. Coux O, Tanaka, Goldberg AL. 1996. Structure and functions of the 20S and 26S proteasomes. Annu. Rev. Biochem. 65: 801-847. https://doi.org/10.1146/annurev.bi.65.070196.004101
  12. Pommier Y, Leteurtre F, Fesen MR, Fujimori A, Bertrand R, Solary E, et al. 1994. Cellular determinants of sensitivity and resistance to DNA topoisomerase inhibitors. Cancer Invest. 12: 530-542. https://doi.org/10.3109/07357909409021413
  13. Wang JC. 1996. DNA topoisomerases. Annu. Rev. Biochem. 65: 635-692. https://doi.org/10.1146/annurev.bi.65.070196.003223
  14. Froelich-Ammon SJ, Osheroff N. 1995. Topoisomerase poisons: harnessing the dark side of enzyme mechanism. J. Biol. Chem. 270: 21429-21432. https://doi.org/10.1074/jbc.270.37.21429
  15. Ma Y, Hendershot LM. 2001. The unfolding tale of the unfolded protein pesponse. Cell 107: 827-830. https://doi.org/10.1016/S0092-8674(01)00623-7
  16. Mazzarella RA, Green M. 1987. Erp99, and abundant, conserved glycoprotein of the endoplasmic reticulum, is homologous to 90 kDa heat shock protein (hsp90) and the 94 kDa glucose-regulated protein (GRP94). J. Biol. Chem. 262: 74-108.
  17. Choo SJ, Park HR, Ryoo IJ, Kim JP, Yun BS, Kim CJ, et al. 2005. Deoxyverrucosidin, a novel GRP78/BiP down-regulator, produced by Penicillium sp. J. Antibiot. 58: 210-213. https://doi.org/10.1038/ja.2005.26
  18. Yu JL, Coomber BL, Kerbel RS. 2002. A paradigm for therapy-induced microenvironmental changes in solid tumors leading to drug resistance. Differentiation 70: 599-609. https://doi.org/10.1046/j.1432-0436.2002.700913.x
  19. Fernandez PM, Tabbara SO, Jacobs LK, Manning FCR, Tsangaris TN, Schwarts AM, et al. 2000. Overexpression of the glucose-regulated stress gene GRP78 in malignant but not benign human breast lesions. Breast Cancer Res. Treat. 59: 15-26. https://doi.org/10.1023/A:1006332011207
  20. Umehara K, Nakamura M, Miyase T, Kuroyanagi M, Ueno A. 1996. Studies on differentiation inducers. VI. Lignan derivatives from Arctium fructus. (2). Chem. Pharm. Bull. 44: 2300-2304. https://doi.org/10.1248/cpb.44.2300
  21. Zhao F, Wang L, Liu K. 2009. In vitro anti-inflammatory effects of arctigenin, a lignan from Arctium lappa L., through inhibition on iNOS pathway. J. Ethnopharmacol. 122: 457-462. https://doi.org/10.1016/j.jep.2009.01.038
  22. Zhang N, Wen Q, Ren L, Liang W, Xia Y, Zhang X, et al. 2013. Neuroprotective effect of arctigenin via upregulation of P-CREB in mouse primary neurons and human SH-SY5Y neuroblastoma cells. Int. J. Mol. 14: 18657-18669. https://doi.org/10.3390/ijms140918657
  23. Ogiso Y, Tomida A, Tsuruo T. 2002. Nuclear localization of proteasomes participates in stress-inducible resistance of solid tumor cells to topoisomerase II-directed drugs. Cancer Res. 62: 5008-5012.
  24. Ogiso Y, Tomida A, Lei S, Omura S, Tsuruo T. 2000. Proteasome inhibition circumvents solid tumor resistance to topoisomerase II-directed drugs. Cancer Res. 60: 2429-2434.

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