한국식품영양학회지 (The Korean Journal of Food And Nutrition)

  • 제32권2호
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  • Pages.106-113
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  • 2019
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  • 1225-4339(pISSN)
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  • 2287-4992(eISSN)
한국식품영양학회 (The Korean Society of Food and Nutrition)
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생물전환을 통한 음나무발효물의 LPS에 대한 경쟁적 억제제 효과 및 내독소혈증 억제 효과

The Inhibitory Effect of Fermented Kalopanax pictus by Bioconversion on Endotoxemia and the Competitive Inhibitor Activity on LPS

  • 김성필 ((주)에스티알바이오텍 중앙연구소) ;
  • 이화영 ((주)에스티알바이오텍 중앙연구소) ;
  • 인수아 ((주)에스티알바이오텍 중앙연구소) ;
  • 성은영 ((주)에스티알바이오텍 중앙연구소) ;
  • 김진만 ((주)에스티알바이오텍 중앙연구소) ;
  • 남석현 (아주대학교 생명과학과)
  • 투고 : 2019.03.07
  • 심사 : 2019.03.17
  • 발행 : 2019.04.30
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초록

The objective of this study was to evaluate the effect of fermented Kalopanax pictus (KP-F) on macrophage activation and its effect as a competitive inhibitor of LPS and inhibitory effect on endotoxemia. The results showed that KP-F could activate macrophage in a dose-dependent manner, and KP-F was confirmed to act as a ligand for TLR4. Also, it was found that KP-F did not exhibit the same biotoxicity as LPS in intraperitoneal injection, and that it could suppress the neutrophil migration induced by LPS administration. In normal mice, the body weight, tissue weight, and amount of nitrite and pro-inflammatory cytokines in serum showed no significant changes with KP-F diet for 2 weeks, confirming that administration of KP-F in normal mice did not lead to over activation of immune response and biotoxicity. In the mouse model of endotoxemia induced by LPS and D-galactosamine(D-GalN) in sub-lethal dose, the diet of KP-F effectively inhibited the amount of nitrite and cytokines in the blood, and thus was found to be able to relieve the hepatic and kidney injury. In addition, in the endotoxemia mouse model induced by LPS and D-GalN of lethal dose, the survival rate was increased by KP-F diet in a dose-dependent manner.

키워드

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Fig. 1. Effect of KP-F on production of nitric oxide in macrophage (A) and cell cytotoxicity (B).

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Fig. 2. Effect of KP-F on neutrophil migration induced by LPS treatment.

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Fig. 3. Effect of KP-F on the mortality of the lethal shock in mice challenged by LPS/D-GalN.

Table 1. Body weight and organ weight changes by KP-F administration in normal mice

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Table 2. Nitrite and cytokines production by KP-F administration in normal mice

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Table 3. Effect of KP-F on nitrite and cytokines production in endotoxemia mice induced by LPS/D-GalN

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Table 4. Effect of KP-F on liver injury induced by LPS/D-GalN

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Table 5. Effect of KP-F on kidney injury induced by LPS/D-GalN

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