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Inhibitory Effect of Cymbopogon Citratus Ethanol Extracts on Adipogenesis in 3T3-L1 Preadipocytes

레몬그라스 에탄올 추출물의 3T3-L1 지방세포 분화 억제효과

  • Jo, Yong Seok (Department of Pathology, College of Korean Medicine, Wonkwang University) ;
  • Ju, Sung Min (Department of Pathology, College of Korean Medicine, Wonkwang University) ;
  • Hwang, Keum Hee (Department of Pathology, College of Korean Medicine, Wonkwang University) ;
  • Kim, Min Sook (Hisol Co., Ltd.) ;
  • Kim, Kwang Sang (Hisol Co., Ltd.) ;
  • Jeon, Byung Hun (Department of Pathology, College of Korean Medicine, Wonkwang University)
  • 조용석 (원광대학교 한의과대학 병리학교실) ;
  • 주성민 (원광대학교 한의과대학 병리학교실) ;
  • 황금희 (원광대학교 한의과대학 병리학교실) ;
  • 김민숙 (하이솔) ;
  • 김광상 (하이솔) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실)
  • Received : 2019.01.21
  • Accepted : 2019.02.19
  • Published : 2019.02.25

Abstract

Cymbopogon citratus, commonly know as lemongrass, prossesses strong antioxidant, anti-tumor and anti-inflammatory properties. Howerver, its anti-obesity activity remains to be elucidated. This study investigated the effect of ethanol extract of Cymbopogon citratus on adipogenesis, and its underlying mechanism, in 3T3-L1 preadipocytes. The results demonstrated that ethanol extracts of Cymbopogon citratus effectively suppressed intercellular lipid accumulation at non-toxic concentrations, and was associated with the down-regulation of adipocyte-specific transcription factors, including $C/EBP{\alpha}$ and $PPAR{\gamma}$, and phosphorylation of $AMPK{\alpha}$. Furthermore, ethanol extracts of Cymbopogon citratus increased p21 and p21 expression, while the expression of CDK2, cyclin A and cyclin B1 was reduced. As a result, ethanol extracts of Cymbopogon citratus seems to induce G0/G1 cell cycle arrest of 3T3-L1 cells. On the other hand, ERK and Akt signaling pathways were not involved in anti-adipogenesis by ethanol extracts of Cymbopogon citratus. Taken together, theses results suggest that ethanol extracts of Cymbopogon citratus inhibits adipocyte differentiation in 3T3-L1 cells and can be used as a safe and efficient natural substance to manage anti-obesity.

Keywords

References

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