Fig. 1. HPLC chromatogram of A) standard 5 catechins and caffeine in green tea and of B) whole extract of green tea and C) CGTE.
Fig. 2. Effect of CGTE on cell viability and MMP-1 expression by TNF-α + IFN-γ treatment in HaCaT cells. HaCaT cells were incubated with TNF-α + IFN-γ (10 ng/㎖) in CGTE (10, 50, 100, 200 and 400 ㎍/㎖) for 24 h.
Fig. 3. Effect of CGTE on UVB-induced skin wrinkles, epidermal thickening, and histological changes.
Fig. 4. Effect of CGTE on the UVB-induced skin barrier function perturbation. (A) TEWL and (B) β- glucosidase was measured as a marker of the skin barrier function.
Fig. 5. Effect of CGTE on MMP-2 and MMP-9 mRNA expression in animal skin exposed to UVB.
Table 1. HPLC running condition for tea catechins and extract.
Table 2. The primer sequence used for quantitative real-time PCR.
참고문헌
- Berardesca E and Maibach HI. (1990). Transepidermal water loss and skin surface hydration in the non invasive assessment of stratum corneum function. Dermatosen in Beruf und Umwelt. Occupation and Environment. 38:50-53.
- Boelsma E, Hendriks HF and Roza L. (2001). Nutritional skin care: Health effects of micronutrients and fatty acids. American Journal of Clinical Nutrition. 73:853-864. https://doi.org/10.1093/ajcn/73.5.853
- Cabrera C, Artacho R and Gimenez R. (2006). Beneficial effects of green tea-a review. Journal of the American College of Nutrition. 25:79-99. https://doi.org/10.1080/07315724.2006.10719518
- Chen ZP, Schell JB, Ho CT and Chen KY. (1998). Green tea epigallocatechin gallate shows a pronounced growth inhibitory effect on cancerous cells but not on their normal counterparts. Cancer Letters. 129:173-179. https://doi.org/10.1016/S0304-3835(98)00108-6
- Choi SW and Ryu SY. (2014). Mass production of highly pure epigallocatechin gallate. Korea. Patents. 1016594230000.
- Chung JH. (2003). Photoaging in Asians. Photodermatology, Photoimmunology and Photomedicine. 19:109-121. https://doi.org/10.1034/j.1600-0781.2003.00027.x
- Ha JS and Kim JH. (2005). Protective effect of EGCG against reactive oxygen species-induced stress. International Journal of Oral Biology. 30:77-84.
- Imokawa G, Takema Y, Yorimoto Y, Tsukahara K, Kawai M and Imayama S. (1995). Degree of ultraviolet-induced tortuosity of elastic fibers in rat skin is age dependent. Journal of Investigative Dermatology. 105:254-258. https://doi.org/10.1111/1523-1747.ep12317607
- Kang JH, Pack YK, Chung ST and Row KH. (1999). Extraction and purification of EGCG from green tea. Korean Journal of Biotechnology and Bioengineering. 14:517-522.
- Khan N, Afaq F, Saleem M, Ahmad N and Mukhtar H. (2006). Targeting multiple signaling pathways by green tea polyphenol (-)-epigallocatechin-3-gallate. Cancer Research. 66:2500-2505. https://doi.org/10.1158/0008-5472.CAN-05-3636
- Kondo S. (2000). The roles of cytokines in photoaging. Journal of Dermatological Science. 23:30-36. https://doi.org/10.1016/S0923-1811(99)00076-6
- Lee SJ. (2008). Novel natural products as active material for beauty food. Food Science and Industry. 40:10-18.
- Levin J and Maibach H. (2005). The correlation between transepidermal water loss and percutaneous absorption: An overview. Journal of Controlled Release. 103:291-299. https://doi.org/10.1016/j.jconrel.2004.11.035
- Oh JH, Kim A, Park JM, Kim SH and Chung AS. (2006). Ultraviolet B-induced matrix metalloproteinase-1 and -3 secretion are mediated via PTEN/Akt pathway in human dermal fibroblasts. Journal of Cellular Physiology. 209:775-785. https://doi.org/10.1002/jcp.20754
- Rutter K, Sell D, Fraser N, Obrenovich M, Zito M, Starke-Reed P and Monnier VM. (2003). Green tea extract suppresses the age-related increase in collagen cross-linking and fluorescent products in C57BL/6 mice. International Journal for Vitamin and Nutrition Research. 73:453-460. https://doi.org/10.1024/0300-9831.73.6.453
- Soter NA. (1990). Acute effects of ultraviolet radiation on the skin. Seminars in Dermatology. 9:11-15.
- Steenport M, Khan KM, Du B, Barnhard SE, Dannenberg AJ and Falcone DJ. (2009). Matrix metalloproteinase(MMP)-1 and MMP-3 induce macrophage MMP-9: Evidence for the role of TNF-a and cyclooxygenase-2. Journal of Immunology. 183:8119-8127. https://doi.org/10.4049/jimmunol.0901925
- Youn HS. (2007). Anti-inflammatory effects of resveratrol, (-)-epigallocatechin-3-gallate and curcumin by the modulation of toll like receptor signaling pathways. Korean Journal of Food Science and Technology. 39:481-487.
- Yusuf N, Irby C, Katiyar SK and Elmets CA. (2007). Photoprotective effects of green tea polyphenols. Photodermatology, Photoimmunology and Photomedicine. 23:48-56. https://doi.org/10.1111/j.1600-0781.2007.00262.x