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Inhibition of Osteoclast differentiation based on precipitation time of titanium surfaces immersed in modified simulated body fluid

Modified simulated body fluid에 침전한 티타늄 표면에서 침전 기간에 따라 나타나는 파골 세포의 분화억제 양상

  • Chang, Hyun-min (Department of Prosthodontics, School of Dentistry, Seoul National University) ;
  • Heo, Seong-Joo (Department of Prosthodontics, School of Dentistry, Seoul National University) ;
  • Kim, Seong-Kyun (Department of Prosthodontics, School of Dentistry, Seoul National University) ;
  • Koak, Jai-Young (Department of Prosthodontics, School of Dentistry, Seoul National University)
  • 장현민 (서울대학교 치과대학 치과보철학교실) ;
  • 허성주 (서울대학교 치과대학 치과보철학교실) ;
  • 김성균 (서울대학교 치과대학 치과보철학교실) ;
  • 곽재영 (서울대학교 치과대학 치과보철학교실)
  • Received : 2019.03.25
  • Accepted : 2019.04.18
  • Published : 2019.04.30

Abstract

Purpose: The purpose of this study is to investigate the changes of osteoclast differentiation inhibition according to the period of precipitation when titanium disks were immersed in Modified simulated body fluid (mSBF). Materials and methods: Titanium alloy (Ti grade III) disks with machined surfaces and anodized surfaces were immersed in distilled water and mSBF, respectively. The immersion periods were 7 days, 14 days, 21 days and 28 days, and the control group was immersed in distilled water for each period. RAW 264.7 cells capable of differentiating into osteoclasts were used to measure the number of adherent cells, the measurement of TRAP activity, and the expression pattern of NFATc1 by western blotting. Results: The degree of inhibition of osteoclast differentiation was found to be statistically significant when the disks were immersed in mSBF for more than 14 days on both machined surfaces and anodized surfaces. There was no correlation between immersion time and cell attachment. When the disks were immersed for more than 14 days, TRAP activity was decreased and NFATc1 expression was inhibited. Futhermore, the decrease in TRAP activity and the inhibition of NFATc1 expression remained unchanged. Conclusion: Immersion of titanium disks in mSBF for more than 14 days can prevent RAW 264.7 cells from differentiating into osteoclasts. Inhibition activity does not change even if the immersion period is for more than 14 days.

목적: 본 연구의 목적은 티타늄 디스크를 Modified simulated body fluid (mSBF)에 침전시켰을 때, 침전 시킨 기간에 따른 파골 세포 분화 억제 변화 양상을 알아보는 것이다. 재료 및 방법: Machined surface와 anodized surface를 가진 티타늄 합금(Ti grade III)디스크를 각각 증류수와 mSBF에 침전 시켰다. 침전 기간은 7일, 14일, 21일, 28일 진행하였으며, 각각의 기간 동안 대조군은 증류수에 침전하였다. 파골 세포로 분화 가능한 RAW 264.7 세포를 점주하여 침전 기간에 따른 부착된 세포 수 측정, TRAP 활성 측정, western blot을 통한 NFATc1의 발현양상을 측정하였다. 결과: Machined surface와 anodized surface 모두에서 mSBF에14일 이상 침전하였을 때, 파골 세포의 분화를 억제하는 능력이 통계적으로 유의하게 나타났다. 침전 기간과 세포의 부착은 상관관계가 없었다. 14일 이상 침전시켰을 때, TRAP 활성은 감소되었으며, NFATc1의 발현은 억제되었다. 14일 이상 침전 시켰을 때, TRAP활성 감소 및 NFATc1 발현 억제 양상은 변함이 없었다. 결론: 티타늄 합금 디스크를 14일 이상 mSBF에 침전시키면 RAW 264.7 세포가 파골 세포로 분화하는 것을 막을 수 있다. 침전기간이 증가해도 분화 억제 양상은 변화하지 않는다.

Keywords

References

  1. Albrektsson T, Branemark PI, Hansson HA, Kasemo B, Larsson K, Lundstrom I, McQueen DH, Skalak R. The interface zone of inorganic implantsIn vivo: Titanium implants in bone. Ann Biomed Eng 1983;11:1-27. https://doi.org/10.1007/BF02363944
  2. Branemark PI, Adell R, Breine U, Hansson BO, Lindstrom J, Ohlsson A. Intra-osseous anchorage of dental prostheses. I. Experimental studies. Scand J Plast Reconstr Surg 1969;3:81-100. https://doi.org/10.3109/02844316909036699
  3. Albrektsson T, Zarb G, Worthington P, Eriksson AR. The long-term efficacy of currently used dental implants: a review and proposed criteria of success. Int J Oral Maxillofac Implants 1986;1:11-25.
  4. Le Guehennec L, Soueidan A, Layrolle P, Amouriq Y. Surface treatments of titanium dental implants for rapid osseointegration. Dent Mater 2007;23:844-54. https://doi.org/10.1016/j.dental.2006.06.025
  5. de Jonge LT, Leeuwenburgh SC, Wolke JG, Jansen JA. Organic-inorganic surface modifications for titanium implant surfaces. Pharm Res 2008;25:2357-69. https://doi.org/10.1007/s11095-008-9617-0
  6. Lang NP, Salvi GE, Huynh-Ba G, Ivanovski S, Donos N, Bosshardt DD. Early osseointegration to hydrophilic and hydrophobic implant surfaces in humans. Clin Oral Implants Res 2011;22:349-56. https://doi.org/10.1111/j.1600-0501.2011.02172.x
  7. Zhao G, Schwartz Z, Wieland M, Rupp F, Geis-Gerstorfer J, Cochran DL, Boyan BD. High surface energy enhances cell response to titanium substrate microstructure. J Biomed Mater Res A 2005;74:49-58.
  8. Hori N, Ueno T, Suzuki T, Yamada M, Att W, Okada S, Ohno A, Aita H, Kimoto K, Ogawa T. Ultraviolet light treatment for the restoration of age-related degradation of titanium bioactivity. Int J Oral Maxillofac Implants 2010;25:49-62.
  9. Junker R, Dimakis A, Thoneick M, Jansen JA. Effects of implant surface coatings and composition on bone integration: a systematic review. Clin Oral Implants Res 2009;20:185-206. https://doi.org/10.1111/j.1600-0501.2009.01777.x
  10. Kokubo T, Kushitani H, Sakka S, Kitsugi T, Yamamuro T. Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W. J Biomed Mater Res 1990;24:721-34. https://doi.org/10.1002/jbm.820240607
  11. Bohner M, Lemaitre J. Can bioactivity be tested in vitro with SBF solution? Biomaterials 2009;30:2175-9. https://doi.org/10.1016/j.biomaterials.2009.01.008
  12. Kim MH, Lee SY, Kim MJ, Kim SK, Heo SJ, Koak JY. Effect of biomimetic deposition on anodized titanium surfaces. J Dent Res 2011;90:711-6. https://doi.org/10.1177/0022034511400074
  13. Minkin C, Marinho VC. Role of the osteoclast at the boneimplant interface. Adv Dent Res 1999;13:49-56. https://doi.org/10.1177/08959374990130011401
  14. Kim MH, Lee SY, Heo SJ, Kim SK, Kim MJ, Koak JY. Osteoclastic response on titanium surfaces in modified simulated body fluid. Int J Oral Maxillofac Implants 2017;32:337-43. https://doi.org/10.11607/jomi.4654
  15. Hsu H, Lacey DL, Dunstan CR, Solovyev I, Colombero A, Timms E, Tan HL, Elliott G, Kelley MJ, Sarosi I, Wang L, Xia XZ, Elliott R, Chiu L, Black T, Scully S, Capparelli C, Morony S, Shimamoto G, Bass MB, Boyle WJ. Tumor necrosis factor receptor family member RANK mediates osteoclast differentiation and activation induced by osteoprotegerin ligand. Proc Natl Acad Sci USA 1999;96:3540-5. https://doi.org/10.1073/pnas.96.7.3540
  16. Takayanagi H. Osteoimmunological insight into bone damage in rheumatoid arthritis. Mod Rheumatol 2005;15:225-31. https://doi.org/10.3109/s10165-005-0398-6
  17. Asagiri M, Sato K, Usami T, Ochi S, Nishina H, Yoshida H, Morita I, Wagner EF, Mak TW, Serfling E, Takayanagi H. Autoamplification of NFATc1 expression determines its essential role in bone homeostasis. J Exp Med 2005;202:1261-9. https://doi.org/10.1084/jem.20051150