Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

In ovo feeding of creatine pyruvate alters energy metabolism in muscle of embryos and post-hatch broilers

  • Yang, Tong (Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee, College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Zhao, Minmeng (Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee, College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Li, Jiaolong (Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee, College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Zhang, Lin (Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee, College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Jiang, Yun (Ginling College, Nanjing Normal University) ;
  • Zhou, Guanghong (Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee, College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Gao, Feng (Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee, College of Animal Science and Technology, Nanjing Agricultural University)
  • Received : 2018.08.06
  • Accepted : 2018.12.06
  • Published : 2019.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: This study was conducted to investigate the effects of in ovo feeding (IOF) of creatine pyruvate (CrPyr) on the energy metabolism in thigh muscle of embryos and neonatal broilers. Methods: A total of 960 eggs were randomly assigned to three treatments: i) non-injected control group, ii) saline group injected with 0.6 mL of physiological saline (0.75%), and iii) CrPyr group injected with 0.6 mL of physiologi-cal saline (0.75%) containing 12 mg CrPyr/egg on 17.5 d of incubation. After hatching, 120 male chicks (close to the average body weight of the pooled group) in each group were randomly assigned to eight replications. The feeding experiment lasted 7 days. Results: The results showed that IOF of CrPyr increased glucose concentrations in the thigh muscle of broilers on 2 d after injection (p<0.05). Compared with the control and saline groups, the concentration of creatine in CrPyr group was increased on 2 d after injection and the day of hatch (p<0.05). Moreover, IOF of CrPyr increased the creatine kinase activity at hatch and increased the activities of hexokinase and pyruvate kinase on 2 d after injection and the day of hatch (p<0.05). Chicks in CrPyr group showed higher mRNA expressions of glucose transporter 3 (GLUT3) and GLUT8 on the day of hatch (p<0.05). Conclusion: These results demonstrated that IOF of CrPyr was beneficial to enhance muscle energy reserves of em-bryos and hatchlings.

Keywords

References

  1. Shafey TM, Alodan MA, Alruqaie IM, Abouheif MA. In ovo feeding of carbohydrates and incubated at a high incubation temperature on hatchability and glycogen status of chicks. S Afr J Anim Sci 2012;42:210-20.
  2. Jr ME. Nutrition of the developing embryo and hatchling. Poult Sci 2007;86:1043-9. https://doi.org/10.1093/ps/86.5.1043
  3. Yu LL, Gao T, Zhao MM, et al. In ovo feeding of L-arginine alters energy metabolism in post-hatch broilers. Poult Sci 2018; 97:140-8. https://doi.org/10.3382/ps/pex272
  4. Uni YNZ. Early nutritional strategies. Worlds Poult Sci J 2010; 66:639-46. https://doi.org/10.1017/S0043933910000620
  5. Willemsen H, Debonne M, Swennen QEN, et al. Delay in feed access and spread of hatch: importance of early nutrition. Worlds Poult Sci J 2010;66:177-88. https://doi.org/10.1017/S0043933910000243
  6. Lamot DM, van de Linde IB, Molenaar R, et al. Effects of moment of hatch and feed access on chicken development. Poult Sci 2014;93:2604-14. https://doi.org/10.3382/ps.2014-04123
  7. Powell DJ, Velleman SG, Cowieson AJ, Singh M, Muir WI. Influence of chick hatch time and access to feed on broiler muscle development. Poult Sci 2016;95:1433-48. https://doi.org/10.3382/ps/pew047
  8. Uni Z, Ferket PR, Tako E, Kedar O. In ovo feeding improves energy status of late-term chicken embryos. Poult Sci 2005;84:764-70. https://doi.org/10.1093/ps/84.5.764
  9. Kornasio R, Halevy O, Kedar O, Uni Z. Effect of in ovo feeding and its interaction with timing of first feed on glycogen reserves, muscle growth, and body weight. Poult Sci 2011;90:1467-77. https://doi.org/10.3382/ps.2010-01080
  10. Zhang L, Zhu XD, Wang XF, Li JL, Gao F, Zhou GH. Individual and combined effects of in-ovo injection of creatine monohydrate and glucose on somatic characteristics, energy, status, and posthatch performance of broiler embryos and hatchlings. Poult Sci 2016;95:2352-9. https://doi.org/10.3382/ps/pew130
  11. Zhao MM, Gao T, Zhang L, et al. Effects of in ovo feeding of creatine pyruvate on the hatchability, growth performance and energy status in embryos and broiler chickens. Animal 2017;11:1689-97. https://doi.org/10.1017/S1751731117000374
  12. Brosnan JT, Brosnan ME. Creatine: endogenous metabolite, dietary, and therapeutic supplement. Annu Rev Nutr 2007;27: 241-61. https://doi.org/10.1146/annurev.nutr.27.061406.093621
  13. Nabuurs CI, Choe CU, Veltien A, et al. Disturbed energy metabolism and muscular dystrophy caused by pure creatine deficiency are reversible by creatine intake. J Physiol 2013;591:571-92. https://doi.org/10.1113/jphysiol.2012.241760
  14. Allen PJ. Creatine metabolism and psychiatric disorders: does creatine supplementation have therapeutic value? Neurosci Biobehav Rev 2012;36:1442-62. https://doi.org/10.1016/j.neubiorev.2012.03.005
  15. Chen J, Wang M, Kong Y, Ma H, Zou S. Comparison of the novel compounds creatine and pyruvateon lipid and protein metabolism in broiler chickens. Animal 2011;5:1082-9. https://doi.org/10.1017/S1751731111000085
  16. Chen J, Huang J, Deng J, Ma H, Zou S. Use of comparative proteomics to identify the effects of creatine pyruvate on lipid and protein metabolism in broiler chickens. Vet J 2012;193:514-21. https://doi.org/10.1016/j.tvjl.2012.01.034
  17. Li JL, Guo ZY, Li YJ, Zhang L, Gao F, Zhou GH. Effect of creatine monohydrate supplementation on carcass traits, meat quality and postmortem energy metabolism of finishing pigs. Anim Prod Sci 2015;56:48-54. https://doi.org/10.1071/AN14017
  18. Panserat S, Medale F, Blin C, et al. Hepatic glucokinase is induced by dietary carbohydrates in rainbow trout, gilthead seabream, and common carp. Am J Physiol Regul Integr Comp Physiol 2000;278:R1164-R70. https://doi.org/10.1152/ajpregu.2000.278.5.R1164
  19. Sekine N, Cirulli V, Regazzi R, et al. Low lactate dehydrogenase and high mitochondrial glycerol phosphate dehydrogenase in pancreatic beta-cells. Potential role in nutrient sensing. J Biol Chem 1994;269:4895-902. https://doi.org/10.1016/S0021-9258(17)37629-9
  20. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the $2^{-{{\Delta}}{{\Delta}}CT}$ method. Methods 2001;25:402-8. https://doi.org/10.1006/meth.2001.1262
  21. Foye OT, Ferket PR, Uni Z. Ontogeny of energy and carbohydrate utilisation of the precocial avian embryo and hatchling. Avian Poult Biol Rev 2007;18:93-101. https://doi.org/10.3184/147020607X296033
  22. Oliveira JED, Uni Z, Ferket PR. Important metabolic pathways in poultry embryos prior to hatch. Worlds Poult Sci J 2008;64:488-99. https://doi.org/10.1017/S0043933908000160
  23. Wang XF, Zhu XD, Li YJ, et al. Effect of dietary creatine monohydrate supplementation on muscle lipid peroxidation and antioxidant capacity of transported broilers in summer. Poult Sci 2015;94:2797-804. https://doi.org/10.3382/ps/pev255
  24. Zhang L, Yue HY, Zhang HJ, et al. Transport stress in broilers: I. Blood metabolism, glycolytic potential, and meat quality. Poult Sci 2009;88:2033-41. https://doi.org/10.3382/ps.2009-00128
  25. Kono T, Nishida M, Nishiki Y, Seki Y, Sato K, Akiba Y. Characterisation of glucose transporter (GLUT) gene ex-pression in broiler chickens. Br Poult Sci 2005;46:510-5. https://doi.org/10.1080/00071660500181289
  26. Ju JS, Smith JL, Oppelt PJ, Fisher JS. Creatine feeding increases GLUT4 expression in rat skeletal muscle. Am J Physiol Endocrinol Metab 2005;288:e347-e52. https://doi.org/10.1152/ajpendo.00238.2004
  27. Seki Y, Kan S, Akiba Y. Changes in muscle mrnas for hexokinase, phosphofructokinase-1 and glycogen synthase in acute and persistent hypoglycemia induced by tolbutamide in chickens. Comp Biochem Physiol B Biochem Mol Biol 2005;142:201-8. https://doi.org/10.1016/j.cbpc.2005.07.004
  28. Tako E. Effects of in ovo feeding of carbohydrates and betahydroxy-beta-methylbutyrate on the development of chicken intestine. Poult Sci 2004;83:2023-8. https://doi.org/10.1093/ps/83.12.2023
  29. Irimia JM, Rovira J, Nielsen JN, Guerrero M, Wojtaszewski JFP, Cusso R. Hexokinase 2, glycogen synthase and phosphorylase play a key role in muscle glycogen supercompensation. PLos One 2012;7:e42453. https://doi.org/10.1371/journal.pone.0042453
  30. Foye OT, Uni Z, Mcmurtry JP, Ferket PR. The effects of amniotic nutrient administration, "in ovo feeding" of arginine and/or ${\beta}$-hydroxy-${\beta}$-methyl butyrate (HMB) on insulin-like growth factors, energy metabolism and growth in turkey poults. Int J Poult Sci 2006;5:309-17. https://doi.org/10.3923/ijps.2006.309.317
  31. Tangara M, Chen W, Xu J, Huang FR, Peng J. Effects of in ovo feeding of carbohydrates and arginine on hatchability, body weight, energy metabolism and perinatal growth in duck embryos and neonates. Br Poult Sci 2010;51:602-8. https://doi.org/10.1080/00071668.2010.520303
  32. Rayasam GV, Tulasi VK, Sodhi R, Davis JA, Ray A. Glycogen synthase kinase 3: more than a namesake. Br J Pharmacol 2009;156:885-98. https://doi.org/10.1111/j.1476-5381.2008.00085.x
  33. Roach PJ, Depaoli-Roach AA, Hurley TD, Tagliabracci VS. Glycogen and its metabolism: some new develop-ments and old themes. Biochem J 2012;441:763-87. https://doi.org/10.1042/BJ20111416

Cited by

  1. Early Nutrition Programming ( in ovo and Post-hatch Feeding) as a Strategy to Modulate Gut Health of Poultry vol.6, 2019, https://doi.org/10.3389/fvets.2019.00082