한국고유종 점줄종개 Cobitis nalbanti의 난발생 및 초기생활사

Egg Development and Early Life History of the Korean Endemic Sand Spine Loach, Cobitis nalbanti (Pisces: Cobitidae)

한국고유종 점줄종개 Cobitis nalbanti의 난발생 및 초기생활사를 조사하였다. 성어는 2011년 6월 영산강 상류인 전남 장성군 북하면 성암리에서 족대를 이용하여 채집하였다. 채집된 성숙한 친어는 복강에 Ovaprim을 주사하고, 12시간 경과 후 복부압박법으로 채란하고 건식법으로 인공수정시켰다. 산란된 성숙란은 약한 점착성을 띤 밝은 황색의 분리침성난으로 난경은 $0.99{\pm}0.03mm$이고 산란수는 $1,527{\pm}410$개였다. 수온 $25^{\circ}C$에서 수정 후 52시간 후에 50%가 부화하였으며, 크기는 전장 $4.2{\pm}0.22mm$였다. 부화 5일 후에 전장 $6.0{\pm}0.34mm$로 난황이 모두 흡수되어 후기자어로 이행하였으며, 15일 후에는 전장 $10.8{\pm}0.45mm$로 지느러미 기조가 모두 정수로 되어 치어기로 이행하였다. 부화 후 100일 후에는 전장 $41.1{\pm}2.95mm$로 4개의 점줄로 이루어진 Gambetta's zone이 뚜렷하고 외형은 성어와 비교적 유사하였다.

Egg development and early life history of the Korean endemic sand spine loach, Cobitis nalbanti, were investigated in the present study. Adult fish were sampled using spoon nets at the Yeongsan River in Seongam-ri, Bukha-myeon, Jangseong-gun, Jeollanam-do, Korea, June 2011. Eggs were obtained after injecting Ovarprim into females. Eggs were then artificially fertilized using the dry method in the laboratory. Mature eggs were transparent and slightly adhesive with light yellowish coloring, measured $0.99{\pm}0.03mm$ ($mean{\pm}SD$) in diameter. And number of spawned eggs were $1,527{\pm}410$ per individual. Hatching (50%) of the embryo occurred 52 hours after fertilization a water temperature of $25^{\circ}C$, and the average newly hatched larvae size was about $4.2{\pm}0.22mm$ in total length. At fifth day after hatching, the larval total length reached $6.0{\pm}0.34mm$ on average and their yolk sac had been completely absorbed. At fifteen day after hatching, larva entered the juvenile stage and reached $10.8{\pm}0.45mm$ in total length. At 100th day after hatching, the formation of Gambetta's zone of four dotted line was complete and juveniles were similar in general appearance to adults, and they averaged $41.1{\pm}2.95mm$ in total length.