Journal of Plant Biotechnology

  • 제46권1호
  • /
  • Pages.9-16
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  • 2019
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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철피석곡의 기내 Protocorm Like Bodys(PLBs) 재증식 및 신초형성에 미치는 생장조절제 및 탄소원의 영향

Effect of plant growth regulators and carbon sources on proliferation and shoot formation of PLBs in Dendrobium candidum

  • 장지우 (순천대학교 농업교육과) ;
  • 김창길 (경북대학교 원예과학과) ;
  • ;
  • 이도진 (순천대학교 농업교육과) ;
  • 정미영 (순천대학교 농업교육과)
  • Jang, Jee-woo (Department of Agricultural Education, Sunchon National University) ;
  • Kim, Chang Kil (Department of Horticultural Science, Kyungpook National University) ;
  • Trinh, Ngoc Ai (School of Agriculture and Aquaculture, Tra Vinh University) ;
  • Lee, Do-Jin (Department of Agricultural Education, Sunchon National University) ;
  • Chung, Mi Young (Department of Agricultural Education, Sunchon National University)
  • 투고 : 2018.11.05
  • 심사 : 2018.12.10
  • 발행 : 2019.03.31
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초록

본 연구는 철피석곡의 PLB 기내 대량증식 방법에 대한 기초자료를 제공함으로써 다양한 분야의 소재활용 수요를 충족시키고 아울러 멸종위기에 처해 있는 철피석곡을 보존하고 수행하였다. 종자유래의 PLB 재 증식에는 H3P4 기본배지에 NAA $0.1mg{\cdot}L^{-1}$과 kinetin $0.1mg{\cdot}L^{-1}$를 혼용한 배지가 가장 적합하였다. H3P4 기본배지 내 탄소원으로 sucrose $10g{\cdot}L^{-1}$를 첨가 했을 때 PLB의 생장이 가장 양호하였다. 재 증식된 PLB로부터 shoot 형성율은 1/4MS 또는 H1P2 배지에 sucrose를 $10g{\cdot}L^{-1}$ 첨가한 배지에서 가장 높았고 shoot의 초장도 길어졌다.

Dendrobium candidum Wallich ex Lindley is a traditional Chinese medicine plant and has been widely used for medicinal and ornamental purposes. In this study, several different factors affecting micro propagation of protocorm-like bodies (PLBs) such as basal media, plant growth regulators, and carbon sources. The proliferation PLB derived from seeds was the best in $H_3P_4$ basal medium containing $0.1mg{\cdot}L^{-1}$ NAA and $0.1mg{\cdot}L^{-1}$ Kinetin. PLB growth was the best when $10g{\cdot}L^{-1}$ sucrose was added to the carbon atoms in the medium. The rate of shoot formation from the propagated PLB was the highest in 1/4 MS or $H_1P_2$ medium containing $10g{\cdot}L^{-1}$ sucrose, and the shoot length was longer than the others.

키워드

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