Mycobiology

The Korean Society of Mycology (한국균학회)
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Anti-Melanogenic Effect from Submerged Mycelial Cultures of Ganoderma weberianum

  • Lai, Ying-Jang (Department of Food Science, National Quemoy University) ;
  • Hsu, Kai-Di (Institute of Biotechnology, National Taiwan University) ;
  • Huang, Tzu-Jung (Graduate Institute of Food Science Technology, National Taiwan University) ;
  • Hsieh, Chang-Wei (Department of Food Science and Biotechnology, National Chung Hsing University) ;
  • Chan, Yu-Hin (Institute of Biotechnology, National Taiwan University) ;
  • Cheng, Kuan-Chen (Institute of Biotechnology, National Taiwan University)
  • Received : 2018.11.02
  • Accepted : 2019.01.02
  • Published : 2019.03.01
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Abstract

Compounds from Lingzhi has been demonstrated the ability for inhibiting tyrosinase (a key enzyme in melanogenesis) activity. In this study, we investigated the anti-melanogenic activity from the submerged mycelial culture of Ganoderma weberianum and elucidated the skin lightening mechanism by B16-F10 murine melanoma cells. From the cellular context, several fractionated mycelium samples exhibited anti-melanogenic activity by reducing more than 40% extracellular melanin content of B16-F10 melanoma cells. In particular, the fractionated chloroform extract (CF-F3) inhibited both secreted and intracellular melanin with the lowest dosage (25 ppm). Further analysis demonstrated that CF-F3 inhibited cellular tyrosinase activity without altering its protein expression. Taken together, our study has demonstrated that the chemical extracts from submerged mycelial culture of G. weberianum have the potential to serve as an alternative anti-melanogenic agent.

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References

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