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토양에서 분리한 국내 미기록종 Pseudomonas 속 6종의 생화학적 특성과 계통 분류

Isolation and characterization of 6 unrecorded Pseudomonas spp. from Korean soil

  • 김현중 (경희대학교 생명과학대학 식품생명공학 전공) ;
  • 정유정 (국립생물자원관 생물자원연구부 미생물자원과) ;
  • 김해영 (경희대학교 생명과학대학 식품생명공학 전공) ;
  • 허문석 (국립생물자원관 생물자원연구부 미생물자원과)
  • Kim, Hyun-Joong (Institute of Life Sciences and Resources Graduate School of Biotechnology, Kyung Hee University) ;
  • Jung, You-Jung (Biological Resources Research Department, National Institute of Biological Resources) ;
  • Kim, Hae-Yeong (Institute of Life Sciences and Resources Graduate School of Biotechnology, Kyung Hee University) ;
  • Hur, Moonsuk (Biological Resources Research Department, National Institute of Biological Resources)
  • 투고 : 2018.11.30
  • 심사 : 2018.12.19
  • 발행 : 2019.03.31

초록

생명공학산업의 주요한 자원인 미생물의 가치는 날로 증대하고 있으며, 이로 인해 생물자원을 확보하기 위한 국가간의 경쟁은 심화되고 있다. 이에 다양한 환경에서 미생물자원을 발굴하고 특성을 확인하는 것은 미래의 잠재적 생물자원을 확보하는데 큰 의미가 있다. 본 연구에서는 Pseudomonas속에 속하는 6종의 미생물들을 일반 토양에서 분리하여 분자계통학적 분석을 통해 국내 미기록종임을 확인하였다. 본 연구로 확보된 미생물들은 자생 생물자원의 다양성을 늘리고, 산업계에 생물자원의 선택의 폭을 늘리는데 도움이 될 것으로 기대된다.

In 2017, as a study to discover indigenous prokaryotic species in Korea, a total of 6 bacterial strains assigned to the genus Pseudomonas were isolated from soil. From the high 16S rRNA gene sequence similarity (${\geq}99.5%$) and phylogenetic analysis with closely related species, the isolated strains were identified as independent Pseudomonas species which were unrecorded in Korea. The six Pseudomonas species were Pseudomonas mandelii, P. canadensis, P. thivervalensis, P. jessenii, P. lurida, and P. brenneri. Gram reaction, culture conditions, colony and cell morphology, basic physiological and biochemical characteristics are described in the species description section.

키워드

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Fig. 1. Phylogenetic tree of Pseudomonas spp. based on the 16S rRNA gene sequence analysis using the neighbor-joining method showing the position of the newly isolated Pseudomonas strains (highlighted in bold) compared with the closely reference Pseudomonas strains.

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Fig. 2. Transmission electron micrograph of isolated Pseudomonas spp.

Table 1. Pseudomonas strains isolated from soil in this study

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Table 2. Physiological and biochemical characteristics and differential carbohydrate assimilation patterns of isolated Pseudomonas spp. using API 20NE (2.1) kit, API ZYM kit, and API 32GN kit

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