Asian-Australasian Journal of Animal Sciences

  • 제32권4호
  • /
  • Pages.477-484
  • /
  • 2019
  • /
  • 1011-2367(pISSN)
  • /
  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
권호 표지
DOI QR코드

DOI QR Code

Hair follicle development and related gene and protein expression of skins in Rex rabbits during the first 8 weeks of life

  • Wu, Zhenyu (College of Animal Science and Technology, Shandong Agricultural University) ;
  • Sun, Liangzhan (College of Animal Science and Technology, Shandong Agricultural University) ;
  • Liu, Gongyan (College of Animal Science and Technology, Shandong Agricultural University) ;
  • Liu, Hongli (College of Animal Science and Technology, Shandong Agricultural University) ;
  • Liu, Hanzhong (Sichuan Academy of Grassland Sciences) ;
  • Yu, Zhiju (Sichuan Academy of Grassland Sciences) ;
  • Xu, Shuang (College of Animal Science and Technology, China Agricultural University) ;
  • Li, Fuchang (College of Animal Science and Technology, Shandong Agricultural University) ;
  • Qin, Yinghe (College of Animal Science and Technology, China Agricultural University)
  • 투고 : 2018.03.30
  • 심사 : 2018.09.13
  • 발행 : 2019.04.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: We aimed to observe hair follicle (HF) development in the dorsal skin and elucidate the expression patterns of genes and proteins related to skin and HF development in Rex rabbits from birth to 8 weeks of age. Methods: Whole-skin samples were obtained from the backs of Rex rabbits at 0, 2, 4, 6, and 8 weeks of age, the morphological development of primary and secondary HFs was observed, and the gene transcript levels of insulin-like growth factor-I (IGF-I), epidermal growth factor (EGF), bone morphogenetic protein 2 (BMP2), transforming growth factor ${\beta}-1$, 2, and 3 ($TGF{\beta}-1$, $TGF{\beta}-2$, and $TGF{\beta}-3$) were examined using quantitative real-time polymerase chain reaction (PCR). Additionally, Wnt family member 10b (Wnt10b) and ${\beta}$-Catenin gene and protein expression were examined by quantitative real-time PCR and western blot, respectively. Results: The results showed significant changes in the differentiation of primary and secondary HFs in Rex rabbits during their first 8 weeks of life. The IGF-I, EGF, $TGF{\beta}-2$, and $TGF{\beta}-3$ transcript levels in the rabbits were significantly lower at 2 weeks of age than at birth and gradually increased thereafter, while the BMP2 and $TGF{\beta}-1$ transcript levels at 2 weeks of age were significantly higher than those at birth and gradually decreased thereafter. ${\beta}$-Catenin gene expression was also significantly affected by age, while the Wnt10b transcript level was not. However, the Wnt10b and ${\beta}$-catenin protein expression levels were the lowest at 2 and 4 weeks of age. Conclusion: Our data showed that a series of changes in HFs in dorsal skin occurred during the first 8 weeks. Many genes, such as IGF-I, EGF, BMP2, $TGF{\beta}-1$, $TGF{\beta}-2$, $TGF{\beta}-3$, and ${\beta}$-Catenin, participated in this process, and the related proteins Wnt10b and ${\beta}$-Catenin in skin were also affected by age.

키워드

참고문헌

  1. Alonso L, Fuchs E. The hair cycle. J Cell Sci 2006;119:391-3. https://doi.org/10.1242/jcs.02793
  2. Galbraith H. Fundamental hair follicle biology and fine fibre production in animals. Animal 2010;4:1490-509. https://doi.org/10.1017/S175173111000025X
  3. Zhu B, Xu T, Yuan J, Guo X, Liu D. Transcriptome sequencing reveals differences between primary and secondary hair folliclederived dermal papilla cells of the Cashmere goat (Capra hircus). PloS one 2013;8: e76282. https://doi.org/10.1371/journal.pone.0076282
  4. Moore GPM, Panaretto BA, Robertson D. Effects of epidermal growth factor on hair growth in the mouse. J Endocrinol 1981;88:293-9. https://doi.org/10.1677/joe.0.0880293
  5. Moore GPM, Panaretto BA, Robertson D. Epidermal growth factor delays the development of the epidermis and hair follicles of mice during growth of the first coat. Anat Rec 1983;205:47-55. https://doi.org/10.1002/ar.1092050107
  6. Philpott MP, Sanders DA, Kealey T. Effects of insulin and insulin-like growth factors on cultured human hair follicles: IGF-I at physiologic concentrations is an important regulator of hair follicle growth in vitro. J Invest Dermatol 1994;102:857-61. https://doi.org/10.1111/1523-1747.ep12382494
  7. Little JC, Westgate GE, Evans A, Granger SP. Cytokine gene expression in intact anagen rat hair follicles. J Invest Dermatol 1994;103:715-20. https://doi.org/10.1111/1523-1747.ep12398584
  8. Bierie B, Moses HL. Tumour microenvironment: $TGF{\beta}$: The molecular Jekyll and Hyde of cancer. Nat Rev Cancer 2006;6:506-20. https://doi.org/10.1038/nrc1926
  9. Massague J. $TGF{\beta}$ in cancer. Cell 2008;134:215-30. https://doi.org/10.1016/j.cell.2008.07.001
  10. Lonn P, Moren A, Raja E, Dahl M, Moustakas A. Regulating the stability of $TGF{\beta}$ receptors and Smads. Cell Res 2009;19:21-35. https://doi.org/10.1038/cr.2008.308
  11. Huelsken J, Vogel R, Erdmann B, et al. ${\beta}$-catenin controls hair follicle morphogenesis and stem cell differentiation in the skin. Cell 2001;105:533-45. https://doi.org/10.1016/S0092-8674(01)00336-1
  12. Ouji Y, Yoshikawa M, Moriya K, et al. Effects of Wnt-10b on hair shaft growth in hair follicle cultures. Biochem Biophys Res Commun 2007;359:516-22. https://doi.org/10.1016/j.bbrc.2007.05.135
  13. Ouji Y, Yoshikawa M, Moriya K, et al. Wnt-10b, uniquely among Wnts, promotes epithelial differentiation and shaft growth. Biochem Biophys Res Commun 2008;367:299-304. https://doi.org/10.1016/j.bbrc.2007.12.091
  14. Katoh M, Katoh M. WNT signaling pathway and stem cell signaling network. Clin Cancer Res 2007;13:4042-5. https://doi.org/10.1158/1078-0432.CCR-06-2316
  15. De Blas C, Wiseman J. Nutrition of the rabbit. Oxfordshire, UK: CABI; 2010; 324 p.
  16. Millar SE. Molecular mechanisms regulating hair follicle development. J Invest Dermatol 2002;118:216-25. https://doi.org/10.1046/j.0022-202x.2001.01670.x
  17. Botchkarev VA, Paus R. Molecular biology of hair morphogenesis: development and cycling. J Exp Zool Part B Mol Dev Evol 2003;298:164-80. https://doi.org/10.1002/jez.b.33
  18. Rudman SM, Philpott MP, Thomas GA, Kealey T. The role of IGF-I in human skin and its appendages: morphogen as well as mitogen? J Invest Dermatol 1997;109:770-7. https://doi.org/10.1111/1523-1747.ep12340934
  19. Hansen LA, Alexander N, Hogan ME, et al. Genetically null mice reveal a central role for epidermal growth factor receptor in the differentiation of the hair follicle and normal hair development. Am J Pathol 1997;150:1959-75.
  20. Plikus MV, Baker RE, Chen CC, et al. Self-organizing and stochastic behaviors during the regeneration of hair stem cells. Science 2011;332:586-9. https://doi.org/10.1126/science.1201647
  21. Andl T, Reddy ST, Gaddapara T, Millar SE. WNT signals are required for the initiation of hair follicle development. Dev Cell 2002;2:643-53. https://doi.org/10.1016/S1534-5807(02)00167-3
  22. Lei MX, Chuong CM, Widelitz RB. Tuning Wnt signals for more or fewer hairs. J Invest Dermatol 2013;133:7-9. https://doi.org/10.1038/jid.2012.446
  23. Li YH, Zhang K, Ye JX, et al. Wnt10b promotes growth of hair follicles via a canonical Wnt signalling pathway. Clin Exp Dermatol 2011;36:534-40. https://doi.org/10.1111/j.1365-2230.2011.04019.x
  24. Li YH, Zhang K, Yang K, et al. Adenovirus-mediated Wnt10b overexpression induces hair follicle regeneration. J Invest Dermatol 2013;133:42-8. https://doi.org/10.1038/jid.2012.235
  25. Stromberg S, Bjorklund MG, Asplund C, et al. A high-throughput strategy for protein profiling in cell microarrays using automated image analysis. Proteomics 2007;7:2142-50. https://doi.org/10.1002/pmic.200700199

피인용 문헌

  1. Deoxynivalenol Induces Inflammation in the Small Intestine of Weaned Rabbits by Activating Mitogen-Activated Protein Kinase Signaling vol.8, 2019, https://doi.org/10.3389/fvets.2021.632599
  2. Rabbits – their domestication and molecular genetics of hair coat development and quality vol.52, pp.1, 2021, https://doi.org/10.1111/age.13024
  3. Effect of Clostridium on proliferating cell nuclear antigen and ghrelin in the small intestine of fattening pigs fed with deoxynivalenol vol.14, pp.1, 2019, https://doi.org/10.3920/wmj2020.2569
  4. Acetate stimulates lipogenesis via AMPKα signaling in rabbit adipose-derived stem cells vol.303, 2019, https://doi.org/10.1016/j.ygcen.2021.113715