문화기술의 융합 (The Journal of the Convergence on Culture Technology)

  • 제5권1호
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  • Pages.409-412
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  • 2019
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  • 2384-0358(pISSN)
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  • 2384-0366(eISSN)
국제문화기술진흥원 (The International Promotion Agency of Culture Technology)
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Cefotaxime 처리를 통한 칼라 기내 식물체의 오염 감소 효과

Effect of cefotaxime on reduction of contamination for callus tissues in calla 'Gagsi'

  • 이상희 (건국대학교 생명공학과) ;
  • 김영진 (강원도농업기술원 원예연구과) ;
  • 양환래 (건국대학교 생명공학과) ;
  • 김종보 (건국대학교 생명공학과)
  • 투고 : 2018.10.18
  • 심사 : 2018.12.27
  • 발행 : 2019.02.28
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초록

본 실험은 칼라 'Gagsi' 품종의 줄기를 절편체로 사용하여 칼라의 대량증식 시스템을 확립하고자 진행이 되었다. 실험은 cefotaxime 항생제 (25, 50, 100 mg/L)가 포함된 Murashige와 Skoog (MS) 기본 배지를 사용하였다. 캘러스 유도는 MS 기본 배지에 NAA 0.5 mg/L, BA 1.0 mg/L 등의 식물 호르몬을 첨가한 배지가 사용되었다. 기본 MS 배지에 cefotaxime 25, 50, 100 mg/L 농도가 첨가 된 배지에서 비교한 결과 캘러스 유도율은 25 mg/L 농도에서 10.5 %가 나와 가장 효율이 높았고 갈변율은 10.5 %로 가장 낮았다. 캘러스 유도 배지인 MNB에 cefotaxime 25 mg/L을 첨가한 배지에서 캘러스 유도율은 34.5 %, 갈변율은 27.0 %가 나타났다. 항생제인 cefotaxime은 이전에 식물 조직 배양 실험에서도 사용되었고, 실험 결과 칼라 식물 생장을 촉진시키는 효과와 오염 및 갈변을 억제하는 긍정적인 영향을 준다는 것을 입증하였다. 이를 토대로 대량 증식 시스템 개발에 기여를 할 수 있고 농가 소득 향상에 도움이 될 것으로 판단된다.

We investigated the development of a micropropagation protocol for multiplication of calla 'Gagsi' by using shoots as explant. The callus was induced on Murashige and Skoog (MS) basal medium containing cefotaxime antibiotics (25, 50, 100 mg/L). Also, MS basal medium with NAA 0.5 mg/L and BA 1.0 mg/L was used. The callus induction and browning rates were compared by treatment supplemented cefotaxime 25, 50 and 100 mg/L in basal MS medium. The callus induction rate was 10.5 % and browning rate was also, 10.5 % on the MS containing 25 mg/L. In the MNB containing cefotaxime, the callus induction rate was 34.5 % and browning rate was 27.0 %. The cefotaxime experiment has been widely used in previous studies. It is thought that it will help establish the mass multiplication system by positively affecting the growth and browning reduction of calla plants.

키워드

GJMGCK_2019_v5n1_409_f0001.png 이미지

그림 1. Cefotaxime 첨가 배지에서 ‘각시’ 캘러스 배양 8주 후 캘러스 유도 및 갈변율 비교 Figure 1. Callus formation on various cefotaxime containing medium type in calla ‘Gagsi’. Data were collected after 8 weeks of culture. (A) Callus induced in stem explant by MS. (B) Callus induced in stem explant by MS containing cefotaxime 25 mg/L. (C) Callus induced in stem explant by MS containing cefotaxime 50 mg/L. (D) Callus induced in stem explant by MS containing cefotaxime 100 mg/L. (E) Callus induced in stem explant by MS containing NAA 0.5 mg/L and BA 1.0 mg/L. (F) Callus induced in stem explant by NAA 0.5 mg/L, BA 1.0 mg/L and cefotaxime 25 mg/L medium.

표 1. ‘각시’에서 cefotaxime 처리에 따른 캘러스 형성과 갈변율 Table 1. Formation of callus and browning rate on cefotaxime medium in calla ‘Gagsi’

GJMGCK_2019_v5n1_409_t0001.png 이미지

참고문헌

  1. Kim GM, "A study on product image analysis and design expression using flowers," The Journal of the Convergence on Culture Technology, Vol. 4(1), pp. 231-236, 2018 https://doi.org/10.17703/JCCT.2018.4.1.231
  2. Tija B, "Growth regulator effect on growth an flowering of Zantedeschia rehmannii hybrid," HoriSci, Vol. 22, pp. 507-508, 1987
  3. Corr BE, Widmer RE, "Paclobutrazol, gibberellic acid, and rhizome size affect growth and flowering of Zantedeschia," HoriSci, Vol. 22, pp. 133-135, 1991 https://doi.org/10.21273/HORTSCI.26.2.133
  4. Lee YS, "Micropropagation by apical meristem culture of colored Calla lily (Zantedeschia spp.) and effects on the bulb development of nutriculture of tissue-cultured plantlets," MS thesis, Chonbuk National Univ., 1996
  5. Funnell KA, "Zantedeschia," The physiology of flower bulbs, Vol. 36, pp. 683-704, 1993
  6. Mori G, Kubo T and Yamaguchi T, "Effect of growing temperature and growth medium on the outbreak of bacterial of bacterial soft rot in the production for tubers of Zantedeschia Araceae," Environment Control in Biology (Japan), Vol. 37, pp. 96-96, 1999
  7. Murashige T and Skoog F, "A revised medium for rapid growth and bioassays with tobacco tissue culture," Physiol Planta, Vol. 15, pp. 473-497, 1962 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  8. Pollick K, Barfield DG and Shields R, "The toxicity of antibiotics to plant cell culture," Plant Cell Rep, Vol. 2, pp. 36-39, 1983 https://doi.org/10.1007/BF00269232
  9. Mathias RJ and Boyd LA, "Cefotaxime stimulates callus growth, embryogenesis and regeneration in hexaploid bread wheat (Triticum aestivum Lem, thell), Plant Science, Vol. 46, pp. 217-223 https://doi.org/10.1016/0168-9452(86)90195-0
  10. Mathias RJ and Mukasa C, "The effect of cefotaxime on the growth and regeneration of callus from four varieties of barley (Hordeum vulgare L.)," Plant Cell Rep, Vol. 6, pp. 454-457 https://doi.org/10.1007/BF00272781