DOI QR코드

DOI QR Code

Effects of Bamboo Salt with Sodium Fluoride on the Prevention of Dental Caries

  • Lee, Hye-Jin (Department of Dental Hygiene, Howon University) ;
  • Park, A-Reum (Department of Dental Hygiene, Wonkwang Health Science University) ;
  • Oh, Han-Na (Department of Dental Hygiene, Wonkwang Health Science University)
  • Received : 2019.11.05
  • Accepted : 2019.12.09
  • Published : 2019.12.31

Abstract

Background: Dental caries is one of several prevalent oral diseases caused by dental plaque biofilms. This study evaluated the anti-cariogenic effects of a bamboo salt (BS) and sodium fluoride (NaF) mixture on oral bacteria. Methods: The effects of several mixtures of NaF and BS on acid production, growth, and adhesion to glass beads of Streptococcus mutans, and their anti-cariogenic properties were investigated. The growth of S. mutans was measured according to optical density at 3, 6, 9, 12, 15, 18, and 24 hours after treatment using spectrophotometry at a wavelength of 600 nm, while pH was measured using a pH meter. Adhesion of S. mutans was measured according to the weight of glass beads from each group before and after incubation. Gene expression was measured using real-time polymerase chain reaction. Acid production and growth patterns of S. mutans were compared using repeated measures analysis of variance, followed by Scheffe's post-hoc test. The Kruskal-Wallis test was used to compare adhesion, followed by the Mann-Whitney test. Gene expression in the experimental and control samples was compared using the Student's t-test. Results: Growth, acid production, and adhesion of S. mutans were inhibited in all experimental groups. Expression of gft and fructosyltransferase in S. mutans was inhibited in all groups. A mixture of NaF and BS significantly reduced growth, acid production, adhesion, and gene expression of S. mutans compared with the other groups. Conclusion: Results of the present study demonstrated that a mixture of NaF and BS was useful as a mouth rinse in preventing dental caries.

Keywords

References

  1. Kuramitsu HK: Virulence properties of oral bacteria: impact of molecular biology. Curr Issues Mol Biol 3: 35-36, 2001.
  2. Kuramitsu HK: Virulence factors of mutans streptococci: role of molecular genetics. Crit Rev Oral Biol Med 4: 159-176, 1993. https://doi.org/10.1177/10454411930040020201
  3. Shin HY, Lee EH, Kim CY, et al.: Anti-inflammatory activity of Korean folk medicine purple bamboo salt. Immunopharmacol Immunotoxicol 25: 377-384, 2003. https://doi.org/10.1081/iph-120024505
  4. Chung SY, Yi SY, Rheu HM, et al.: Pharmacological evaluation of bamboo salt. Biomol Ther 7: 178-184, 1999.
  5. Choi CH, Lee HJ, Oh HN, Hong SJ: Evaluation of the remineralization effect of bamboo salt and sodium fluoride solutions. J Korean Acad Oral Health 36: 7-12, 2012.
  6. Petersen FC, Assev S, Scheie AA: Combined effects of NaF and SLS on acid- and polysaccharide-formation of biofilm and planktonic cells. Arch Oral Biol 51: 665-671, 2006. https://doi.org/10.1016/j.archoralbio.2006.02.003
  7. Filoche SK, Soma K, Sissons CH: Antimicrobial effects of essential oils in combination with chlorhexidine digluconate. Oral Microbiol Immunol 20: 221-225, 2005. https://doi.org/10.1111/j.1399-302X.2005.00216.x
  8. Takarada K, Kimizuka R, Takahashi N, Honma K, Okuda K, Kato T: A comparison of the antibacterial efficacies of essential oils against oral pathogens. Oral Microbiol Immunol 19: 61-64, 2004. https://doi.org/10.1046/j.0902-0055.2003.00111.x
  9. Oh HN, Choi CH: Effect of bamboo salt on the caries activity of Streptococcus mutans. J Korean Acad Oral Health 40: 238-243, 2016. https://doi.org/10.11149/jkaoh.2016.40.4.238
  10. Park YN: The effect of erythritol on gtf and ftf expression from Streptococcus mutans. Unpublished doctoral dissertation, Chonnam National University, Gwangju, 2011.
  11. Tarsi R, Muzzarelli RA, Guzman CA, Pruzzo C: Inhibition of Streptococcus mutans adsorption to hydroxyapatite by low-molecular-weight chitosans. J Dent Res 76: 665-672, 1997. https://doi.org/10.1177/00220345970760020701
  12. Baehni PC, Takeuchi Y: Anti-plaque agents in the prevention of biofilm-associated oral diseases. Oral Dis 9 Suppl 1: 23-29, 2003. https://doi.org/10.1034/j.1601-0825.9.s1.5.x
  13. Ooshima T, Minami T, Aono W, et al.: Oolong tea polyphenols inhibit experimental dental caries in SPF rats infected with mutans streptococci. Caries Res 27: 124-129, 1993. https://doi.org/10.1159/000261529
  14. Scheie AA: Modes of action of currently known chemical anti-plaque agents other than chlorhexidine. J Dent Res 68: 1609-1616, 1989.
  15. Kim YH, Ryu HI: Elements in a bamboo salt and comparision of its elemental contents with those in other salts. Yakhak Hoeji 47: 135-141, 2003.
  16. Bradshaw DJ, Marsh PD, Hodgson RJ, Visser JM: Effects of glucose and fluoride on competition and metabolism within in vitro dental bacterial communities and biofilms. Caries Res 36: 81-86, 2002. https://doi.org/10.1159/000057864
  17. Kuramitsu HK, He X, Lux R, Anderson MH, Shi W: Interspecies interactions within oral microbial communities. Microbiol Mol Biol Rev 71: 653-670, 2007. https://doi.org/10.1128/MMBR.00024-07
  18. Yamashita Y, Bowen WH, Burne RA, Kuramitsu HK: Role of the Streptococcus mutans gtf genes in caries induction in the specific-pathogen-free rat model. Infect Immun 61: 3811-3817, 1993. https://doi.org/10.1128/iai.61.9.3811-3817.1993