Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지
DOI QR코드

DOI QR Code

Whitening and anti-wrinkle effect of Spirodela polyrhiza extracts

부평초 추출물의 미백 및 항주름 효과

  • Kim, Dong Hee (National Institute For Korean Medicine Development) ;
  • Park, Tae Soon (National Institute For Korean Medicine Development) ;
  • Kim, Se Gie (Department of Pharmaceutical Engineering, Daegu Catholic University)
  • Received : 2019.10.17
  • Accepted : 2019.10.29
  • Published : 2019.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The antioxidant, whitening, and anti-wrinkle activity of Spirodela polyrhiza extracts and fractions were evaluated to determine its efficacy as a functional cosmetic material. 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid radical scavenging activities were 44.2 and 74.3%, respectively, at 100 ㎍/mL of SE-E (the ethyl acetate fraction of 70% ethanol extract). To measure anti-wrinkle effects, procollagen biosynthesis and matrix metalloproteinase-1 (MMP-1) inhibition activity were determined. At 25 ㎍/mL of SE (70% ethanol extract), the biosynthesis activity was 48.5%, and SE-E showed the best activity (57.8%) at the same concentration. MMP-1 inhibition activity of SE and SE-E was 13.4 and 28.5%, respectively, at 25 ig/mL. Finally, the inhibition of cellular melanin synthesis and cellular tyrosinase were measured to determine the whitening effect; at 25 ㎍/mL, the inhibition activities of SE were 9.6 and 13.8%, respectively, and those for SE-E were 15.4 and 22.0%, respectively. Our results confirmed the possibility of SE and SE-E as effective functional materials. Further research investigating the antimicrobial, anti-inflammatory, and anticancer activities of S. polyrhiza is necessary to confirm its potential use in the food, cosmetics, and drug industries.

부평초 추출물 및 부평초 분획물의 기능성 화장품 소재로서의 효능을 측정하기 위하여 항산화, 항주름 및 미백 활성을 평가하였다. DPPH와 ABTS+ 라디칼 소거 활성은 부평초 70% 에탄올추출물의 에틸아세테이트 분획물(SE-E) 100 ㎍/mL 농도에서 각각 44.2와 74.3%로 나타났다. 항주름 활성을 측정하기 위하여 세포내에서의 procollagen 생합성 활성과 MMP-1 저해 활성을 측정하였다. 그 결과 부평초 70% 에탄올추출물(SE)은 25 ㎍/mL 농도에서 48.5%, 같은 농도에서 SE-E는 가장 높은 57.8%의 생합성 활성을 나타내었다. SE와 SE-E의 MMP-1 저해활성은 25 ㎍/mL 농도에서 각각 13.4와 28.5%로 나타났다. 마지막으로 미백 효과를 검증하기 위해 melanin 생합성 저해 활성과 세포내 tyrosinase 저해 활성을 측정한 결과, 25 ㎍/mL 농도에서 SE는 각각 9.6과 13.8%의 저해활성을 나타내었으며 SE-E는 각각 15.4와 22.0%의 값을 나타내었다. 본 연구의 결과로부터 SE와 SE-E의 높은 항주름 및 미백 활성이 확인되어 우수한 기능성 소재로 개발 가능성이 높아 졌으며 추가적인 항균, 항염, 항암 등의 연구가 진행된다면 제약, 화장품, 기능성 식품 등의 분야에 효과적인 소재로 활용될 수 있을 것으로 사료된다.

Keywords

References

  1. Zukiewicz-Sobczak WA, Adamczuk P, Wroblewska P, Zwolinski J, Chmielewska-Badora J, Krasowska E, Galinska EM, Cholewa G, Piatek J, Kozlik J (2013) Allergy to Selected Cosmetic Ingredients. Postep Derm Alergol 30: 307-310
  2. Amasa W, Santiago D, Mekonen S, Ambelu A (2012) Are Cosmetics Used in Developing Countries Safe?. J Toxicology 2012: 8-15
  3. Juliano C, Magrini GA (2018) Cosmetic Functional Ingredients from Botanical Sources for Anti-Pollution Skincare Products. Cosmetics 5: 19 https://doi.org/10.3390/cosmetics5010019
  4. Kanlayavattanakula M, Louritha N, Chaikula P (2016) Jasmine Rice Panicle: A Safe and Efficient Natural Ingredient for Skin Aging Treatments. J Ethnopharmacology 193: 607-616 https://doi.org/10.1016/j.jep.2016.10.013
  5. Svobodova A, Walterova D, Vostalova J (2006) Ultraviolet Light Induced Alteration to the Skin. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 150: 25-38 https://doi.org/10.5507/bp.2006.003
  6. Roy A, Sahu RK, Matlam M, Deshmukh VK, Dwivedi J, Jha AK (2013) In vitro Techniques to Assess the Proficiency of Skin Care Cosmetic Formulations. Pharmacognosy Reviews 7: 97-106 https://doi.org/10.4103/0973-7847.120507
  7. Lin JW, Chiang HM, Lin YC, Wen KC (2008) Natural Products with Skin-whitening Effects. J Food and Drug Analysis 16: 1-10
  8. Smit N, Vicanova J, Pavel S (2009) The Hunt for Natural Skin Whitening Agents. Int J Mol Sci 10: 5326 https://doi.org/10.3390/ijms10125326
  9. Gilchrest BA (1989) Skin aging and photoaging: an overview. J Am Acad Dermatol 2: 610-613 https://doi.org/10.1016/S0190-9622(89)70227-9
  10. Kang KS, Kim ID, Kwon RH, Heo YY, Oh SH, Kim MA, Jung HJ, Kang HY, Ha BJ (2007) The evaluation of anti-wrinkle effects in oriental herb extract. J Life Sci 17: 1147-1151 https://doi.org/10.5352/JLS.2007.17.8.1147
  11. Lee BG, Kim JH, Ham SG, Lee CE (2014) Study on biological activities of extracts for cosmeceutical development from Lagerstroemia indica L. branch. Korean J Plant Res 27: 29-34 https://doi.org/10.7732/kjpr.2014.27.1.029
  12. Kim DS, Kim DH, Oh MJ, Lee KG, Kook MC, Park CS (2010) Antiaging and whitening activities of ethanol extract of Yuza (Citrus junos SIEB ex TANAKA) by-product. J Soc Cosmet Scientists Korea 36: 137-143
  13. Suh SS, Shin JS (1969) Studies on phytosterols. Yakhakhoe Chi 13: 144-146
  14. Chi HJ (1981) Irridoid-containing Korean Medicinal plants (I). Kor J Pharmacogn 12(1): 19-22
  15. Harborne JB (1986) The natural distribution in angiosperms of anthocyanins acylated with aliphatic dicarboxylic acids. Phytochemistry 25: 1887-1894 https://doi.org/10.1016/S0031-9422(00)81168-1
  16. Xue Q, Wen-ni H, Cheng X, Jian H, Li-jun W, De-an G, Min Y (2011) Qualitative and quantitative analysis of flavonoids in Spirodela polyrhiza by high-performance liquid chromatography coupled with mass spectrometry. Phytochem Anal 22: 475-483 https://doi.org/10.1002/pca.1303
  17. Kim JP, Lee IS, Seo JJ, Jung MY, Kim YH, Y NH, Bae KH (2010) Vitexin, orientin and other flavonoids from Spirodela polyrhiza inhibit adipogenesis in 3T3-L1 cells. Phytother Res 24: 1543-1548 https://doi.org/10.1002/ptr.3186
  18. Cho SE, Kim BW, Kwon KR, Rhim TJ, Kim DH (2008) The effect of Spirodelae herba pharmacopuncture on adipocyte metabolism, Kor Pharmacopuncture Institute 11(1): 71-82 https://doi.org/10.3831/KPI.2008.11.1.071
  19. Lee HJ, Kim MH, Choi YY, Kim EH, Hong JK, Kim KS, Yang WM (2016) Improvement of atopic dermatitis with topical application of Spirodela polyrhiza, J Ethnopharmacology 180: 12-17 https://doi.org/10.1016/j.jep.2016.01.010
  20. Ahn YS, Seong NS, Ham IH, Cho HY (2004) Study on the effect of medicinal herbs used as Bu-pyung (S. polyrhiza and L. paucicostata) on immune and anti-cancer. Kor J Herbology 19: 117-127
  21. Blois MS (1958) Antioxidant activity determination by the use of a stable free radical. Nature 181: 1199-1200 https://doi.org/10.1038/1811199a0
  22. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidnt activity applying and improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  23. Park JG, Kramer BS, Steinberg SM, Carmichael J, Collins JM, Minna JD, Gazdar AF (1987) Chemosensitivity testing of human colorectal carcinoma cell lines using a tetrazolium-based colorimetric assay. Cancer Res 22: 5875-5879
  24. Hosoi J, Abe E, Suda T, Kuroki T (1985) Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res 45: 1474-1478
  25. Choi SY, Cho HS, Sung NJ (2006) The antioxidative and nitrite scavenging ability of solvent extracts from wild grape (Vitis Coignetiea) skin. J Korean Soc Food Sci Nutr 35: 961-966 https://doi.org/10.3746/JKFN.2006.35.8.961
  26. Min KJ, Song JW, Cha CG (2008) The antioxidative and antitumor activity of extracts of Agrimonia pilosa. J Fd Hyg Safety 23: 149-156
  27. Kim MS, Kim KH, Jo JE, Choi JJ, Kim YJ, Kim JH, Jang SA, Yook HS (2011) Antioxidative and antimicrobial activities of Aruncus dioicus var. kamtschaticus Hara extracts. J Korean Soc Food Sci Nutr 40: 47-55 https://doi.org/10.3746/JKFN.2011.40.1.047
  28. Parfitt AM, Simon LS, Villanueva AR, Krane SM (1987) Procollagen type I carboxy-terminal extension peptide in serum as a marker of collagen biosynthesis in bone. correlation with iliac bone formation rates and comparison with total alkaline phosphatase. J Bone Miner Res 2: 427-436 https://doi.org/10.1002/jbmr.5650020510
  29. Koo BS, Hwang EI, So SH, Lee SK, Han GH, Kim NM (2007) Effect of Torilis Fructus on Procollagen Biosynthesis and Activity of Matrix Metalloproteinase-I (MMP-1) in Human Dermal Fibroblast. Kor J Pharmacogn 38(4): 349-353
  30. Fisher GJ, Voorhees JJ (1999) Molecular mechanism of photoaging in human skin in vivo and their prevention by all-trans retinoic acid. Photochem Photobiol, 69: 154-158 https://doi.org/10.1111/j.1751-1097.1999.tb03268.x
  31. Fanger GR, Johnson NL, Johnson GL (1997) MEK kinases are regulated by EGF and selectively interact with Rac/Cdc42. EMBO J 16: 4961-4972 https://doi.org/10.1093/emboj/16.16.4961
  32. Park SH, Lee KH, Han CS, Kim KH, Kim YH (2010) Inhibitory Effects of Carex humilis Extract on Elastase Activity and Matrix Metalloproteinase-1 Expression. J Soc Cosmet Scientists Korea 36: 129-136
  33. Lin CB, Babiarz L, Liebel F, Roydon Price E, Kizoulis M, Gendi menico GJ, Fisher DE, Seiberg M (2002) Modulation of microphthal miaassociated transcription factor gene expression alters skin pigmentation. J Invest Dermatol 119: 1330-1340 https://doi.org/10.1046/j.1523-1747.2002.19615.x
  34. Ando S, Ando O, Suemoto Y, Mishima Y (1993) Tyrosinase gene transcription and its control by melanogenic inhibitors. J Invest Dermatol 100(2 Suppl): 150S-155S https://doi.org/10.1038/jid.1993.68
  35. Cho YH (2008) Inhibitory Effect of Enteromorpha linza on the Melanogenesis in B16 Melanoma Cells. Kor J Pharmacogn 39: 174-178
  36. Jeon MJ, Kim MH, Jang HJ, Lee SW, Kim JH, Kim HS, Lee SH (2012) Whitening effect of Hizikia fusiformis ethanol extract and its fractions. J Life Sci 22: 889-896 https://doi.org/10.5352/JLS.2012.22.7.889
  37. Abdel-Malek ZA, Swope VB, Suzuki I, Harriger D, Boyce ST, Urabe K, Hearing VJ (1995) Mitogenic and melanogenic stimulation of normal human melanocytes by melanotropic peptides. Proc Natl Acad Sci USA 92: 1789-1793 https://doi.org/10.1073/pnas.92.5.1789
  38. Jimenez CC (2004) Inhibition of melanogenesis in response to oxidative stress: transient down regulation of melanocyte differentiation markers and possible involvement of microphthalmia transcription factor. J. Cell Sci 114: 2335-2344 https://doi.org/10.1242/jcs.114.12.2335

Cited by

  1. 대산(大蒜)을 포함하는 복합발효물의 에틸아세테이트 분획으로부터 Tyrosinase 저해활성 성분의 분리 및 동정 vol.33, pp.2, 2019, https://doi.org/10.7732/kjpr.2020.33.2.063
  2. 흰민들레(Taraxacum coreanum)에서 분리한 taraxinic acid의 tyrosinase 활성저해 및 melanin 생성저해 효과 vol.34, pp.4, 2019, https://doi.org/10.7732/kjpr.2021.34.4.368