Mycobiology

  • 제47권4호
  • /
  • Pages.527-532
  • /
  • 2019
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  • 1229-8093(pISSN)
  • /
  • 2092-9323(eISSN)
The Korean Society of Mycology (한국균학회)
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Molecular Characterization of 170 New gDNA-SSR Markers for Genetic Diversity in Button Mushroom (Agaricus bisporus)

  • An, Hyejin (Department of Industrial Plant Science and Technology, Chungbuk National University,) ;
  • Jo, Ick-Hyun (Department of Herbal Crop Research, Rural Development Administration, National Institute of Horticultural and Herbal Science) ;
  • Oh, Youn-Lee (Department of Herbal Crop Research, Rural Development Administration, National Institute of Horticultural and Herbal Science) ;
  • Jang, Kab-Yeul (Department of Herbal Crop Research, Rural Development Administration, National Institute of Horticultural and Herbal Science) ;
  • Kong, Won-Sik (Department of Herbal Crop Research, Rural Development Administration, National Institute of Horticultural and Herbal Science) ;
  • Sung, Jwa-Kyung (Department of Crop Science, Chungbuk National University) ;
  • So, Yoon-Sup (Department of Crop Science, Chungbuk National University) ;
  • Chung, Jong-Wook (Department of Industrial Plant Science and Technology, Chungbuk National University,)
  • 투고 : 2019.04.16
  • 심사 : 2019.09.03
  • 발행 : 2019.12.01
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초록

We designed 170 new simple sequence repeat (SSR) markers based on the whole-genome sequence data of button mushroom (Agaricus bisporus), and selected 121 polymorphic markers. A total of 121 polymorphic markers, the average major allele frequency (MAF) and the average number of alleles (NA) were 0.50 and 5.47, respectively. The average number of genotypes (NG), observed heterozygosity (HO), expected heterozygosity (HE), and polymorphic information content (PIC) were 6.177, 0.227, 0.619, and 0.569, respectively. Pearson's correlation coefficient showed that MAF was negatively correlated with NG (-0.683), NA (-0.600), HO (-0.584), and PIC (-0.941). NG, NA, HO, and PIC were positively correlated with other polymorphic parameters except for MAF. UPGMA clustering showed that 26 A. bisporus accessions were classified into 3 groups, and each accession was differentiated. The 121 SSR markers should facilitate the use of molecular markers in button mushroom breeding and genetic studies.

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참고문헌

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피인용 문헌

  1. SSR 마커를 이용한 유럽 양송이 자원의 유전적 다양성 및 집단구조분석 vol.18, pp.4, 2019, https://doi.org/10.14480/jm.2020.18.4.323
  2. Development of CAPS Markers for Evaluation of Genetic Diversity and Population Structure in the Germplasm of Button Mushroom (Agaricus bisporus) vol.7, pp.5, 2019, https://doi.org/10.3390/jof7050375
  3. Evaluation of Genetic Diversity and Population Structure Analysis among Germplasm of Agaricus bisporus by SSR Markers vol.49, pp.4, 2019, https://doi.org/10.1080/12298093.2021.1940746