The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Functional Genomic Analysis of Bacillus thuringiensis C25 Reveals the Potential Genes Regulating Antifungal Activity against Rosellinia necatrix

Bacillus thuringiensis C25의 흰날개무늬병 Rosellinia necatrix에 대한 항진균 활성에 관여하는 유전자 특성 및 기능 유전체학적 연구

  • Kim, Kangmin (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Lee, Hwa-Yong (Department of Biology, Chungbuk National University) ;
  • Bae, Wonsil (Department of Biology, Chungbuk National University) ;
  • Cho, Min (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Ryu, Hojin (Department of Biology, Chungbuk National University)
  • 김강민 (전북대학교 생명공학부) ;
  • 이화용 (충북대학교 생물학과) ;
  • 배원실 (충북대학교 생물학과) ;
  • 조민 (전북대학교 생명공학부) ;
  • 류호진 (충북대학교 생물학과)
  • Received : 2019.10.02
  • Accepted : 2019.12.03
  • Published : 2019.12.31
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Abstract

Biocontrol agents (BCAs) are widely used to protect plants from diverse biotic and abiotic stresses in agricultural and ecological fields. Among the various microbes, many subspecies of the gram-positive genus, Bacillus, have been successfully industrialized as eco-friendly biological pesticides and fertilizers. In the current study, we demonstrated that Bacillus thuringiensis C25 exhibited antagonistic effects on the mycelial growth of Rosellinia necatrix, a fungal phytopathogen. Scanning electron microscopy analysis revealed that B. thuringiensis C25 degraded the cell wall structures of R. necatrix mycelia. In the functional genomic analysis of B. thuringiensis C25, we annotated 5,683 genes and selected the gene sets that potentially encoded fungal cell wall degrading enzymes (CWDEs). The growth inhibition effects on R. necatrix were highly correlated with the transcriptional activity of the mycelial cell wall degrading genes of B. thuringiensis C25. The transcript levels of CWDEs, including CshiA, B, and Glycos_transf_2 genes in B. thuringiensis C25, were enhanced following co-cultivation with R. necatrix. In conclusion, our study suggested that B. thuringiensis C25 could serve as a suitable candidate for controlling R. necatrix and could facilitate elucidating the mechanisms underlying the antifungal activities of BCAs against phytopathogens.

다양한 생물적 스트레스로부터 경제적으로 중요한 식물을 보호하기 위한 친환경 biocontrol agents (BCAs)는 오늘날 농업 및 생태 분야에서 다양하게 사용되고 있다. 다양한 BCAs 중에서, 많은 그람 양성Bacillus 속 아종이 친환경 생물학적 살충제 및 비료로서 성공적으로 산업화되었다. 이 연구에서 우리는 식물병원성 균류의 균사생장에 길항 효과를 보여주는 BCAs 중 하나인 Bacillus thuringiensis C25가 흰날개무늬병을 일으키는Rosellinia necatrix에 대한 길항작용이 있음을 확인하였다. 주사전자현미경을 통해B. thuringiensis C25가R. necatrix의 균사 세포벽을 분해하여 균사생장을 억제하는 것을 확인하였다. B. thuringiensis C25의 전장 유전체에서 5,683 유전자 세트의 서열을 동정하였고, 잠재적으로 곰팡이 세포벽 분해 효소 (CWDE)를 암호화하는 유전자 세트를 선발하였다. R. necatrix에 대한 균사성장 억제효과는B. thuringiensis C25의 균사 세포벽 분해 유전자의 전사 활성과 높은 상관 관계가 있었다. 상세하게는, B. thuringiensis C25에서ChiA, B 및 Glycos_transf_2 유전자를 포함하는 CWDE의 전사체 수준은R. necatrix와의 공동 배양에 의해 향상되었다. 결론적으로, 본 연구에서는 B. thuringiensis C25가 R. necatrix를 제어할 수 있는 생물학적 소재가 될 수 있으며, 식물 병원체에 대한 BCA의 항진균성 메커니즘의 이해를 촉진할 수 있음을 제시하였다.

Keywords

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