The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Identification and Pathogenicity of Colletotrichum Species Causing Anthracnose of Korean Radish (Rhaphanus sativus) in Korea

무 탄저병을 일으키는 Colletotrichum속의 종 동정 및 병원성

  • Choi, Hyo-Won (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Kim, Jeomsoon (Microbial Safety Team, National Institute of Agricultural Sciences) ;
  • Hong, Sung Kee (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Lee, Young Kee (Crop Protection Division, National Institute of Agricultural Sciences)
  • 최효원 (국립농업과학원 작물보호과) ;
  • 김점순 (국립농업과학원 유해생물팀) ;
  • 홍성기 (국립농업과학원 작물보호과) ;
  • 이영기 (국립농업과학원 작물보호과)
  • Received : 2019.06.07
  • Accepted : 2019.11.14
  • Published : 2019.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

In August 2017, anthracnose symptoms were observed on the petioles and leaf veins of Korean radish (Rhaphanus sativus) in Hongcheon, Jeongseon, and Pyeongchang of the Gangwon province, Korea. Many grayish to dark-brown spots of 1-2 mm in diameter, appeared on the lower surface and leaf veins of the radish leaves. The spots gradually enlarged and coalesced to form dark-brown irregular lesions. Ten Colletotrichum isolates were obtained from the affected tissues of the Korean radish. Out of them, eight isolates were identified as C. higginsianum and two isolates were identified as C. truncatum based on morphological characteristics and multi-locus molecular phylogenetic analysis using the internal transcribed spacers and intervening 5.8S rDNA (ITS), partial beta-tubulin gene (TUB2), partial actin gene (ACT), and partial chitin synthase-1 gene (CHS1). The pathogenicity test was carried out on wounded and unwounded Korean radish (cv. Siraegimu and Osarimu), and Chinese cabbage (cv. Chuno and Smart) by inoculating with a spore suspension. All isolates except one C. truncatum isolate developed typical symptoms on both wounded and unwounded Korean radish. In Chinese cabbage, only the plants inoculated with C. higginsianum isolates developed symptoms regardless of the wound. This is the first report of anthracnose caused by C. truncatum on Korean radish in Korea.

2017년 8월경, 강원도 홍천군, 정선군, 평창군 소재 무 재배 포장에서 잎자루와 잎의 주맥에 형성된 탄저병 병징이 관찰되었다. 회색 내지 짙은 갈색을 띤 1-2 mm 크기의 점무늬가 아래 잎과 잎의 주맥에 형성되었다. 이러한 점무늬는 점차 확대되고 합쳐지면서 갈색을 띤 불규칙한 병반으로 나타났다. 병든 무 잎에서 10개의 Colletotrichum균을 분리하였다. 분리 균주의 형태적 특성과 internal transcribed spacers and intervening 5.8S rDNA (ITS), partial beta-tubulin gene (TUB2), partial actin gene (ACT) 및 partial chitin synthase-1 gene (CHS-1) 부위를 사용한 다자위 분자 계통 분석(multilocus molecular phylogenetic analysis)에 의해 8개 균주는 Colletotrichum higginsianum, 2개 균주는C. truncatum으로 동정되었다. 무(시래기무, 오사리무)와 배추(품종: 추노, 스마트)를 대상으로 상처와 무상처로 구분하여 포자현탁액을 떨어뜨려서 접종하는 방법을 사용하여 병원성 검정을 수행하였다. 무에서는C. truncatum 1개 균주를 제외하고 상처와 무상처 접종에서 모두 병징을 나타냈고, 배추의 경우는C. higginsianum 접종구에서만 상처의 유무에 관계없이 병이 발생하였다. 국내에는 기존에 C. higginsianum은 무와 배추의 탄저병균으로 보고되었으며, C. truncatum에 의한 무 탄저병의 발생은 본 연구에서 국내 최초로 보고한다.

Keywords

References

  1. Seo MS, Chung JH, Park BS, Kim JS. Analysis of surgars content by genotypes in 82 radish(Raphanus sativus L.). Kor J Plant Res 2018;31:453-65. https://doi.org/10.7732/KJPR.2018.31.5.453
  2. The Korean Society of Plant Pathology. List of plant diseases in Korea. 5th ed. Seoul: Korean Society of Plant Pathology; 2009.
  3. Park K, Kim CH. Occurrence of anthracnose on cabbage caused by Colletotrichum dematium. Mycobiology 2001;29:61-2. https://doi.org/10.1080/12298093.2001.12015762
  4. Sato T, Muta T, Imamura Y, Nojima H, Moriwaki J, Yaguchi Y. Anthracnose of Japanese radish caused by Colletotrichum dematium. J Gen Plant Pathol 2005;71:380-3. https://doi.org/10.1007/s10327-005-0214-3
  5. He Y, Chen Q, Shu C, Yang M, Zhou E. Colletotrichum truncatum, a new cause of anthracnose on Chinese flowering cabbage (Brassica parachinensis) in China. Trop Plant Pathol 2016;41:183-92. https://doi.org/10.1007/s40858-016-0086-4
  6. Damm U, O'Connell RJ, Groenewald JZ, Crous PW. The Colletotrichum destructivum species complex - hemibiotrophic pathogens of forage and field crops. Stud Mycol 2014;79:49-84. https://doi.org/10.1016/j.simyco.2014.09.003
  7. Damm U, Woudenberg JHC, Cannon PF, Crous PW. Colletotrichum species with curved conidia from herbaceous hosts. Fungal Divers 2009;39:45-87.
  8. Choi HW, Kim JM, Hong SK, Kim WG, Chun SC, Yu SH. Mating types and optimum culture conditions for sexual state formation of Fusarium fujikuroi isolates. Mycobiology 2009;37:247-50. https://doi.org/10.4489/MYCO.2009.37.4.247
  9. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A guide to methods and application. San Diego:Academic Press; 1990. p. 315-22.
  10. Woudenberg JHC, Aveskamp MM, Gruyter J, Spiers AG, Crous PW. Multiple Didymella teleomorphs are linked to the Phoma clematidina morphotype. Persoonia 2009;22:56-62. https://doi.org/10.3767/003158509X427808
  11. Carbone I, Kohn LM. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 1999;91:553-6. https://doi.org/10.2307/3761358
  12. Sutton BC. The genus Glomerella and anamorph Colletotrichum. In: Bailey JA, Jear MJ, editors. Colletotrichum: biology, pathology and control. Wallingford :CAB International; 1992. p. 1-26.
  13. Hyde KD, Cai L, Cannon PF, Crouch JA, Crous PW, Damm U, Goodwin PH, Chen H, Johnston PR, Jones EBG, et al. Colletotrichum-names in current use. Fungal Divers 2009;39:147-82.
  14. Cannon PF, Damm U, Johnston PR, Weir BS. Colletotrichum-current status and future directions. Stud Mycol 2012;73:181-213. https://doi.org/10.3114/sim0014
  15. Ford R, Banniza S, Photita W, Taylor PWJ. Morphological and molecular discrimination of Colletotrichum truncatum causing anthracnose on lentil in Canada. Australa Plant Path 2004;33:559-69. https://doi.org/10.1071/AP04058