The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Unrecorded Fungal Species Isolated from Medicinal Plant Leaves in Korea

약용식물 잎에서 분리한 국내 미기록 진균

  • 안금란 (단국대학교 미생물학과) ;
  • 노형진 (단국대학교 미생물학과) ;
  • 이동형 (단국대학교 미생물학과) ;
  • 김수산 (단국대학교 미생물학과) ;
  • 김준영 (단국대학교 미생물학과) ;
  • 김성환 (단국대학교 미생물학과)
  • Received : 2019.11.25
  • Accepted : 2019.12.16
  • Published : 2019.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

As an effort to explore fungal diversity, fungal survey was undertaken in 2018 in Jangheung, Korea. For the survey, medicinal plant leaves were sampled from Selaginella tamariscina, Serratula coronata ssp. insularis, Scutellaria baicalensis, Scrophularia buergeriana and subjected to fungal isolation. Four unrecorded fungal species, Paraboeremia selaginellae, Colletotrichum camelliae, Alternaria eichhorniae, and Phomopsis capsici were obtained from the survey. This study described their morphological characteristics including colony features formed on media, light microscopic images and molecular characteristics of nucleotide sequences of the ITS and 28S rDNA regions.

2018년 6월, 전남 장흥군 약초재배사에 재배중인 부처손(Selaginella tamariscina), 산비장이(Serratula coronata ssp. insularis), 황금(Scutellaria baicalensis), 현삼(Scrophularia buergeriana) 등 4종의 약용식물로부터 병 감염 증후가 보이는 잎을 채집하였다. 채집된 잎으로부터 국내 미기록 진균 4종(Paraboeremia selaginellae, Colletotrichum camelliae, Alternaria eichhorniae, Phomopsis capsica)을 분리 동정하여 이들의 형태학적인 특성과 분자생물학적 특성을 기술하였다.

Keywords

References

  1. Biodiversity of the Korean Peninsula (in Korean) [Internet]. National Institute of Biological Resources, Korea; 2019 [cited 2019 Nov 23]. Available from: https://species.nibr.go.kr/index.do.
  2. Korea Forest Seed & Variety Center. Medicinal plants growing on our mountains (in Korean). Korean Forest Service 2013.
  3. Plants For A Future. [cited 2019 Nov 23]. Available from: https://pfaf.org.
  4. Useful tropical plants Database. [cited 2019 Nov 23]. Available from: http://tropical.theferns.info.
  5. Kim GH, Lee SY, Lee AR. The effect of Selaginella tamariscina on inhibition of pancreatic lipase and lipid accumulation. Korean J Food Nutr 2019;32:27-32. https://doi.org/10.9799/KSFAN.2019.32.1.027
  6. Kim GJ, Jung HH, Kim JH, Kim HJ. Indoor air purifying plants. NIHHS 2014.
  7. Odinokov VN, Galyautdinov IV, Nedopekin DV, Khalilov LM, Shashkov AS, Kachala VV, Dinan L, Lafont R. Phytoecdysteroids from the juice of Serratula coronate L. (Asteraceae). Insect Biochem Mol Biol 2002;32:161-5. https://doi.org/10.1016/S0965-1748(01)00106-0
  8. Zhao Q, Chen XY, Martin C. Scutellaria baicalensis, the golden herb from the garden of Chinese medicinal plants. Sci Bull 2016;61:1391-8. https://doi.org/10.1007/s11434-016-1136-5
  9. Wu XM, Zhang LQ, Chen XC, Feng L, Xing WX, Li YM. A new iridoid derivative from the roots of Scrophularia buergeriana. Yao Xue Xue Bao 2014;47:1019-21.
  10. Ahn GR, Choi MA, Kim JE, Seo EJ, Kim JY, Kim SH. A report of eighteen unrecorded fungal species in Korea. Kor J Mycol 2017;45:292-303. https://doi.org/10.4489/KJM.20170037
  11. Choi MA, Park SJ, Ahn GR, Kim SH. Identification and characterization of Paraconiothyrium brasiliense from garden plant Pachysandra terminalis. Kor J Mycol 2014;42:262-8. https://doi.org/10.4489/KJM.2014.42.4.262
  12. Kim SH, Uzunovic A, Breuil C. Rapid detection of Ophiostoma piceace and O. quercus in stained wood by PCR. Appl Environ Microbiol 1999;65:287-90. https://doi.org/10.1128/aem.65.1.287-290.1999
  13. White TJ, Bruns TD, Lee SB, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 315-22.
  14. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 1995;61:1323-30. https://doi.org/10.1128/aem.61.4.1323-1330.1995
  15. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725-9. https://doi.org/10.1093/molbev/mst197
  16. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406-25.
  17. Chen Q, Jiang JR, Zhang GZ, Cai L, Crous PW. Resolving the Phoma enigma. Stud Mycol 2015;82:137-217. https://doi.org/10.1016/j.simyco.2015.10.003
  18. Park MS, Eimes JA, Oh SH, Suh HJ, Oh SY, Lee S, Park KH, Kwon HJ, Kim SY, Lim YW. Diversity of fungi associated with roots of Calanthe orchid species in Korea. J Microbiol 2018;56:49-51. https://doi.org/10.1007/s12275-018-7319-9
  19. Lu Q, Wang Y, Li N, Ni D, Yang Y, Wang X. Differences in the characteristics and pathogenicity of Colletotrichum camelliae and C. fructicola isolated from the tea plant (Camellia sinensis (L.) O. Kuntze). Front Microbiol 2018;9:3060. https://doi.org/10.3389/fmicb.2018.03060
  20. Chen Y, Qiao W, Zeng L, Shen D, Liu Z, Wang X, Tong H. Characterization, pathogenicity and phylogenetic analyses of Colletotrichum species associated with brown blight disease on Camellia sinensis in China. Plant Dis 2017;101:1022-8. https://doi.org/10.1094/PDIS-12-16-1824-RE
  21. Hou LW, Liu F, Duan WJ, Cai L. Colletotrichum aracearum and C. camelliae-japonicae, two holomorphic new species from China and Japan. Mycosphere 2016;7:1111-23. https://doi.org/10.5943/mycosphere/si/2c/4
  22. Lee JK, Cha BJ, Shin HD, La YJ. Forest and shade tree pathology. Hyangmunsa; 2017.
  23. National Species List. [cited 2019 Nov 23]. Available from: http://www.kbr.go.kr/stat/ktsnfiledown/downpopup.do.
  24. Cho WD, Hong SG, Kim WG, Jee HJ, Lee YK, Choi HS, Kim CH. Occurrence and distribution of crop diseases caused by invasive alien pathogens in Korea. Res Plant Dis 2005;11:1-9. https://doi.org/10.5423/RPD.2005.11.1.001
  25. Liu F, Weir BS, Damm U, Crous P, Wang Y, Liu B, Wang M, Zhang M, Cai L. Unravelling Colletotrichum species associated with Camellia: employing ApMat and GS loci to resolve species in the C. gloeosporioides complex. Persoonia 2015;35:63-86. https://doi.org/10.3767/003158515X687597
  26. Nag Raj TR, Ponnappa KM. Blight of waterhyacinth caused by Alternaria eichhorniae sp. Trans Br Mycol Soc 1970;55:123-30. https://doi.org/10.1016/s0007-1536(70)80103-6
  27. Shabana YM, Charudattan R, Elwakil MA. Identification, pathogenicity, and safety of Alternaria eichhorniae from Egypt as a bioherbicide agent for waterhyacinth. Biol Control 1995;5:123-35. https://doi.org/10.1006/bcon.1995.1015
  28. Robeson D, Strobel G, Matusumoto GK, Fisher EL, Chen MH, Clardy J. Alteichin: an unusual phytotoxin from Alternaria eichhorniae, a fungal pathogen of waterhyacinth. Experientia 1984;40:1248-50. https://doi.org/10.1007/BF01946657
  29. Shabana YM, Baka ZAM, Abdel-Fattah GM. Alternaria eichhorniae, a biological control agent for waterhyacinth: mycoherbicidal formulation and physiological and ultrastructural host responses. Eur J Plant Pathol 1997;103:99-111. https://doi.org/10.1023/A:1008698516850
  30. Yirefu F, Lantinga EA, Tessema T. Occurrence and diversity of fungal pathogens associated with water hyacinth and their potential as biocontrol agents in the rift valley of Ethiopia. Int J Pest Manage 2017;63:355-63. https://doi.org/10.1080/09670874.2016.1277046
  31. Uecker FA. A world list of Phomopsis names with notes on nomenclature, morphology and biology. Mycologia memoir, vol 13. Berlin-Suttgart: Cramer Publisher; 1988.
  32. Rodeva R, Karov I, Stoyanova Z, Kovacevik B, Manova V, Georgieva R. Phomopsis capsica and Colletotrichum coccoides infecting pepper in Macedonia. In: International symposium on current trends in plant protection; 2012 Sep 25-28; Belgrade, Serbia. Belgrade: Institute for Plant Protection and Environment; 2012. P. 257-63.