한국식품영양학회지 (The Korean Journal of Food And Nutrition)

  • 제32권6호
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  • Pages.611-619
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  • 2019
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  • 1225-4339(pISSN)
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  • 2287-4992(eISSN)
한국식품영양학회 (The Korean Society of Food and Nutrition)
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더덕 추출물과 용매 분획물의 항산화 및 티로시나아제 저해활성

Antioxidant Activity and Tyrosinase Inhibitory Activities of Codonopsis lanceolata Extract and Solvent Fraction

  • 김지영 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 이민기 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 황병순 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 김기창 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 황인국 (농촌진흥청 국립농업과학원 농식품자원부)
  • Kim, Ji Yeong (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration) ;
  • Lee, Min-Ki (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration) ;
  • Hwang, Byung Soon (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration) ;
  • Kim, Gi-Chang (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration) ;
  • Hwang, In Guk (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration)
  • 투고 : 2019.10.02
  • 심사 : 2019.11.04
  • 발행 : 2019.12.31
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초록

The purpose of this study was to investigate the antioxidant activity and tyrosinase inhibitory activity of Codonopsis lanceolata 50% ethanol extract, and its solvent fractions (n-hexane, ethyl acetate (EA), n-butanol, water). The main components of the EA fraction were qualitatively analyzed using UPLC Q-ToF/MS. Additionally, a quantitative analysis was performed using UPLC. As a result, the total polyphenol content was 113.36 mg gallic acid/g in the EA fraction, which contained the largest amount of the C. lancolata solvent fractions. Also EA showed the highest antioxidant activity than other fractions. The IC50 of DPPH(2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity was 0.03 mg/mL and the IC50 of ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)] radical scavenging activity was 0.049 mg/mL. The EA fraction showed tyrosinase inhibitory activity than other fractions and especially inhibited monophenolase oxidase reaction higher than diphenolase oxidase reaction. The monophenolase oxidase inhibited 55% when the concentration of the EA fraction was 0.25 mg/mL. As a result of Q-ToF/MS analysis, it was confirmed that tangshenoside I and lobetyolin were the main components of EA fraction. Thus, these results suggest that C. lanceolata may be used as a potent source of cosmetic agents.

키워드

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