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Antioxidant Activity and Tyrosinase Inhibitory Activities of Codonopsis lanceolata Extract and Solvent Fraction

더덕 추출물과 용매 분획물의 항산화 및 티로시나아제 저해활성

  • Kim, Ji Yeong (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration) ;
  • Lee, Min-Ki (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration) ;
  • Hwang, Byung Soon (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration) ;
  • Kim, Gi-Chang (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration) ;
  • Hwang, In Guk (Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration)
  • 김지영 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 이민기 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 황병순 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 김기창 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 황인국 (농촌진흥청 국립농업과학원 농식품자원부)
  • Received : 2019.10.02
  • Accepted : 2019.11.04
  • Published : 2019.12.31

Abstract

The purpose of this study was to investigate the antioxidant activity and tyrosinase inhibitory activity of Codonopsis lanceolata 50% ethanol extract, and its solvent fractions (n-hexane, ethyl acetate (EA), n-butanol, water). The main components of the EA fraction were qualitatively analyzed using UPLC Q-ToF/MS. Additionally, a quantitative analysis was performed using UPLC. As a result, the total polyphenol content was 113.36 mg gallic acid/g in the EA fraction, which contained the largest amount of the C. lancolata solvent fractions. Also EA showed the highest antioxidant activity than other fractions. The IC50 of DPPH(2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity was 0.03 mg/mL and the IC50 of ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)] radical scavenging activity was 0.049 mg/mL. The EA fraction showed tyrosinase inhibitory activity than other fractions and especially inhibited monophenolase oxidase reaction higher than diphenolase oxidase reaction. The monophenolase oxidase inhibited 55% when the concentration of the EA fraction was 0.25 mg/mL. As a result of Q-ToF/MS analysis, it was confirmed that tangshenoside I and lobetyolin were the main components of EA fraction. Thus, these results suggest that C. lanceolata may be used as a potent source of cosmetic agents.

Keywords

References

  1. Aga EB, Li HJ, Chen J, Li P. 2012. Chemical constituents from the aerial parts of Codonopsis nervosa. Chin J Nat Med 10:366-369 https://doi.org/10.1016/S1875-5364(12)60073-9
  2. Ahn S, Kim J, Cho H, Park SY, Hwang KT. 2016. Physicochemical and sensory characteristics of hot water extracts of Codonopsis lanceolata root skin and flesh with different heat treatments. Korean J Food Sci Technol 48:104-110 https://doi.org/10.9721/KJFST.2016.48.2.104
  3. Albertazzi P, Steel SA, Clifford E, Bottazzi M. 2002. Attitudes towards and use of dietary supplementation in a sample of postmenopausal women. Climacteric 5:374-382 https://doi.org/10.1080/cmt.5.4.374.382
  4. Ancerewicz J, Migliavacca E, Carrupt PA, Testa B, Bree F, Zini R, Tillement JP, Labidalle S, Guyot D, Chauvet-Monges AM, Crevat A, Ridant AL. 1998. Structure-property relationships of trimetazidine derivatives and model compounds as potential antioxidants. Free Radical Biol Med 25:113-120 https://doi.org/10.1016/S0891-5849(98)00072-0
  5. Boissy RE, Manga P. 2004. On the etiology of contact/occupational vitiligo. Pigment Cell Res 17:208-214 https://doi.org/10.1111/j.1600-0749.2004.00130.x
  6. Chan EWC, Lim YY, Wong LF, Lianto FS, Wong SK, Lim KK, Joe CE, Lim TY. 2008. Antioxidant and tyrosinase inhibition properties of leaves and rhizomes of ginger species. Food Chem 109:477-483 https://doi.org/10.1016/j.foodchem.2008.02.016
  7. Cho EJ, Ju HM, Jeong CH, Eom SH, Heo HJ, Kim DO. 2011. Effect of phenolic extract of dry leaves of Lespedeza cuneata G. Don on antioxidant capacity and tyrosinase inhibition. Korean J Hortic Sci Technol 29:358-365
  8. Choi MH, Kim KH, Yook HS. 2019. Antioxidant and antibacterial activity of premature mandarin. J Korean Soc Food Sci Nutr 48:622-629 https://doi.org/10.3746/jkfn.2019.48.6.622
  9. Du YE, Lee JS, Kim HM, Ahn JH, Jung IH, Ryu JH, Choi JH, Jang DS. 2018. Chemical constituents of the roots of Codonopsis lanceolata. Arch Pharmacal Res 41:1082-1091 https://doi.org/10.1007/s12272-018-1080-9
  10. Hwang BS, Kim JY, Jang M, Kim GC, Park YH, Hwang IG. 2018. Quantitative analysis of tangshenoside I and lobetyolin from Korean deoduk (Codonopsis lanceolata). Korean J Food Nutr 31:957-963 https://doi.org/10.9799/KSFAN.2018.31.6.957
  11. Hwang IG, Woo KS, Kim TM, Kim DJ, Yang MH, Jeong HS. 2006. Change of physicochemical characteristics of Korean pear (Pyrus pyrifolia Nakai) juice with heat treatment conditions. Korean J Food Sci Technol 38:342-347
  12. Ichikawa M, Ohta S, Komoto N, Ushijima M, Kodera Y, Hayama M, Shirato O, Sekita S, Kuroyanagi M. 2009. Simultaneous determination of seven saponins in the roots of Codonopsis lanceolata by liquid chromatography-mass spectrometry. J Nat Med 63:52-57 https://doi.org/10.1007/s11418-008-0294-4
  13. Im DY, Lee KI. 2017. Antioxidative activity and tyrosinase inhibition effect of fractions from the extract of Inonotus obliquus. Korean J Pharmacogn 48:46-50
  14. Jeong MJ, Yang J, Choi WS, Kim JW, Kim SJ, Park MJ. 2017. Chemical compositions and antioxidant activities of essential oil extracted from Neolitsea aciculata (Blume) Koidz leaves. J Korean Wood Sci Technol 45:96-106 https://doi.org/10.5658/WOOD.2017.45.1.96
  15. Jeong SM, Kim SY, Kim IH, Go JS, Kim HR, Jeong JY, Lee HY, Park DS. 2013. Antioxidant activities of processed deoduck (Codonopsis lanceolata) extracts. J Korean Soc Food Sci Nutr 42:924-932 https://doi.org/10.3746/JKFN.2013.42.6.924
  16. Jung YH, Ryu MJ. 2018. Anti-oxidative and anti-inflammatory effects of Codonopsis lanceolata skin extracts. Asian J Beauty Cosmetol 16:347-357 https://doi.org/10.20402/ajbc.2018.0217
  17. Kim JY, Kim JY, Jenis J, Li ZP, Ban YJ, Baiseitova A, Park KH. 2019. Tyrosinase inhibitory study of flavonolignans from the seeds of Silybum marianum (milk thistle). Bioorg Med Chem 27:2499-2507 https://doi.org/10.1016/j.bmc.2019.03.013
  18. Kim WB, Park SH, Hwang HS, Woo JY, Lee HR, Hwang DY, Choi JH, Lee H. 2012. Antioxidative activities and whitening effects of solvent fraction from Prunus davidiana (Carriere) Franch. fruit. J Korean Soc Food Sci Nutr 41:1363-1370 https://doi.org/10.3746/JKFN.2012.41.10.1363
  19. Lee HJ, Kim MJ, Park JH, Park E. 2012. Antioxidative and antigenotoxic activity of white and yellow Chrysanthemum morifolium ramat extracts. J Korean Soc Food Sci Nutr 41:289-294 https://doi.org/10.3746/JKFN.2012.41.3.289
  20. Lee NH, Hong J, Kim JY, Chiang MH. 2009. Antioxidant properties and protective effects of Inula britannica var. chinensis Regel on oxidative stress-induced neuronal cell damage. Korean J Food Sci Technol 41:87-92
  21. Ma XQ, Leung AKM, Chan CL, Su T, Li WD, Li SM, Fong DWF, Yu ZL. 2014. UHPLC UHD Q-TOF MS/MS analysis of the impact of sulfur fumigation on the chemical profile of Codonopsis radix (Dangshen). Analyst 139:505-516 https://doi.org/10.1039/c3an01561k
  22. Mo JH, Kim MA, Kang EJ. 2013. A comparative study of the physiological activities of skin between Codonopsis lanceolata and fermented C. lanceolata extracts as cosmetic ingredient. Korean J Human Beauty Art 14:49-60
  23. Nugroho A, Lim SC, Park HJ. 2012. Qualitative analysis of phenolic substances in artemisia capillaris by LC-MS. Korean J Pharmacogn 43:302-307
  24. Park SY, Lee KJ, Yi TH. 2009. Effect of Buddleja officinalis MAXIM on melanin biosynthesis and anti-oxidative activity. J Beau Trichol 5:33-39
  25. Ryu SH, Jeon YS, Kwon MJ, Moon JW, Lee YS, Moon GS. 1997. Effect of kimchi extracts to reactive oxygen species in skin cell cytotoxicity. J Korean Soc Food Sci Nutr 26:814-821
  26. Seong EH, Lee MJ, Kim H, Shin NR. 2018. Changes of efficacy of antioxidant, antidyslipidemic, antidiabetic and microbiological characteristics in fermented and salt-treated fermented Codonopsis lanceolata. J Korean Med Obes Res 18:106-114 https://doi.org/10.15429/jkomor.2018.18.2.106
  27. Suh JS, Eun JS. 1998. Isolation of active components on immunocytes from Codonopsis lanceolatae. J Nutr Health 31:1076-1081
  28. Tripathi AK, Sama JK, Taneja SC. 2010. An expeditious synthesis of syringaldehyde from para-cresol. Indian J Chem 49B:379-381
  29. Umekawa H, Takada Y, Furuichi Y, Takahashi T, Achiwa Y, Komiya T, Yoshida S. 1999. Inhibition of eukaryotic DNA polymerase alpha by persimmon (Diospyros kaki) extract and related polyphenols. Biochem Mol Biol Int 47:795-801
  30. Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39:44-84 https://doi.org/10.1016/j.biocel.2006.07.001
  31. Wang KH, Lin RD, Hsu FL, Huang YH, Chang HC, Huang CY, Lee MH. 2006. Cosmetic applications of selected traditional Chinese herbal medicines. J Ethnopharmacol 106:353-359 https://doi.org/10.1016/j.jep.2006.01.010
  32. Xia Y, Liu F, Feng F, Liu W. 2017. Characterization, quantitation and similarity evaluation of Codonopsis lanceolata from different regions in China by HPLC-Q-TOF/MS and chemometrics. J Food Compos Anal 62:134-142 https://doi.org/10.1016/j.jfca.2017.05.009
  33. You JK, Chung MJ, Kim DJ, Seo DJ, Park JH, Kim TW, Choe M. 2009. Antioxidant and tyrosinase inhibitory effects of Paeonia suffruticosa water extract. J Korean Soc Food Sci Nutr 38:292-296 https://doi.org/10.3746/JKFN.2009.38.3.292