DOI QR코드

DOI QR Code

Intra-event variability of bacterial composition in stormwater runoff from mixed land use and land cover catchment

  • Paule-Mercado, Ma. Cristina A. (Department of Environmental Engineering and Energy, Myongji University) ;
  • Salim, Imran (Department of Environmental Engineering and Energy, Myongji University) ;
  • Lee, Bum-Yeon (Department of Environmental Engineering and Energy, Myongji University) ;
  • Lee, Chang-Hee (Department of Environmental Engineering and Energy, Myongji University) ;
  • Jahng, Deokjin (Department of Environmental Engineering and Energy, Myongji University)
  • Received : 2018.05.07
  • Accepted : 2018.08.28
  • Published : 2019.01.25

Abstract

Microbial community and composition in stormwater runoff from mixed land use land cover (LULC) catchment with ongoing land development was diverse across the hydrological stage due different environmental parameters (hydrometeorological and physicochemical) and source of runoff. However, limited studies have been made for bacterial composition in this catchment. Therefore, this study aims to: (1) quantify the concentration of fecal indicator bacteria (FIB), stormwater quality and bacterial composition and structure according to hydrological stage; and (2) determine their correlation to environmental parameters. The 454 pyrosequencing was used to determine the bacterial community and composition; while Pearson's correlation was used to determine the correlation among parameters-FIB, stormwater quality, bacterial composition and structure-to environmental parameters. Results demonstrated that the initial and peak runoff has the highest concentration of FIB, stormwater quality and bacterial composition and structure. Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes were dominant bacteria identified in this catchment. Furthermore, the 20 most abundant genera were correlated with runoff duration, average rainfall intensity, runoff volume, runoff flow, temperature, pH, organic matter, nutrients, TSS and turbidity. An increase of FIB and stormwater quality concentration, diversity and richness of bacterial composition and structure in this study was possibly due to leakage from septic tanks, cesspools and latrines; feces of domestic and wild animals; and runoff from forest, destroyed septic system in land development site and urban LULC. Overall, this study will provide an evidence of hydrological stage impacts on the runoff microbiome environment and public health perspective.

Keywords

Acknowledgement

Grant : Development of Integrated Estuarine Management System

Supported by : Ministry of Oceans and Fisheries, Korea Environmental Technology and Industrial Institute

References

  1. American Public Health Association (APHA) (2005), Standard Methods for the Examination of Water and Wastewater, 21st ed., APHA, Washington, DC, U.S.A.
  2. APHA (2009), Standard Methods for the Examination of Water and Wastewater, Revised online ed., APHA, Washington, DC, U.S.A.
  3. Brinkmeyer, R. (2016), "Diversity of bacteria in ships ballast water as revealed by next generation DNA sequencing", Marine Pollut. Bull., 107(1), 277-285. https://doi.org/10.1016/j.marpolbul.2016.03.058
  4. Bushon, R., Brady, A., Christensen, E. and Stelzer, E. (2018), "Multi-year microbial source tracking study characterizing fecal contamination in an urban watershed", Water Environ. Res., 89(2), 127-143.
  5. Cabral, A., Stark, J., Kolm, H. and Martins, C. (2018), "An integrated evaluation of some faecal indicator bacteria (FIB) and chemical markers as potential tools for monitoring sewage contamination in subtropical estuaries", Environ. Pollut., 235, 739-749. https://doi.org/10.1016/j.envpol.2017.12.109
  6. Eren, A.M., Sogin, M. and Maignien, L. (2016), "Editorial: New insights into microbial ecology through subtle nucleotide variation", Front. Microbiol., 7, 1318.
  7. Galfi, H., Osterlund, H., Marsalek, J. and Viklander, M. (2016), "Indicator bacteria and associated water quality constituents in stormwater and snowmelt from four urban catchments", J. Hydrol., 539, 125-140. https://doi.org/10.1016/j.jhydrol.2016.05.006
  8. Huang, H., Chow, C. and Jin, B. (2016), "Characterisation of dissolved organic matter in stormwater using high-performance size exclusion chromatography", J. Environ. Sci., 42, 236-245. https://doi.org/10.1016/j.jes.2015.07.003
  9. Ibekwe, A., Ma, J. and Murinda, S. (2016), "Bacterial community composition and structure in an urban river impacted by different pollutant sources", Sci. Total Environ., 566-567, 1176-1185. https://doi.org/10.1016/j.scitotenv.2016.05.168
  10. Korean Ministry of Environment (2011), Korean Standard Methods for the Examination of Drinking Water, Notice 2011-2021, Korea Ministry of Environment, Republic of Korea.
  11. Leung, H., Chen, G. and Sharma, K. (2005), "Effect of detached/re-suspended solids from sewer sediment on the sewage phase bacterial activity", Water Science and Technology, 52(3), 147-152. https://doi.org/10.2166/wst.2005.0071
  12. Liao, H., Krometis, L.H, Cully Hession, W., Benitez, R., Sawyer, R., Schaberg, E. von Wagoner, E. and Badgley, B.D. (2015), "Storm loads of culturable and molecular fecal indicators in an inland urban stream", Sci. Total Environ., 530-531(15), 347-365. https://doi.org/10.1016/j.scitotenv.2015.05.098
  13. Mcllelan, S., Fisher, J. and Newton, R. (2015), "The microbiome of urban waters", Int. Microbiol., 18, 141-149.
  14. Ministry of the Environment, Korea (MOE) (2014), Yearbook of Statistics Korea, MOE, Republic of Korea.
  15. Paule, M.A., Memon, S.A., Lee, B.Y., Umer, S.R. and Lee, C.H. (2014), "Stormwater runoff quality in correlation to land use and land cover development in Yongin, South Korea", Water Sci. Technol., 70(2), 218-225. https://doi.org/10.2166/wst.2014.207
  16. Paule-Mercado, M.A., Ventura, J.S., Memon, S.A., Jahng, D., Kang, J.H. and Lee, C.H. (2016), "Monitoring and predicting the fecal indicator bacteria concentrations from agricultural, mixed land use and urban stormwater runoff", Sci. Total Environ., 550, 1171-1181. https://doi.org/10.1016/j.scitotenv.2016.01.026
  17. Shanks, O.C., Newton, R.J., Keltya, C.A., Huse, S.M., Sogin, M.L. and McLellan, S.L. (2013), "Comparison of the microbial community structures of untreated wastewaters from different geographic locales", Appl. Environ. Microbiol., 79(9), 2906-2913. https://doi.org/10.1128/AEM.03448-12
  18. Staley, Z., Grabuski, J., Sverko, E. and Edge, T. (2016), "Comparison of microbial and chemical source tracking markers to identify fecal contamination sources in the humber river (Toronto, Ontario, Canada) and associated storm water outfalls", Appl. Environ. Microbiol., 82(21), 6357-6366. https://doi.org/10.1128/AEM.01675-16
  19. Ulrich, N., Rosenberger, A., Brislawn, C., Wright, J., Kessler, C., Toole, D., Solomon, C., Strutt, S., McClure, E. and Lamendella, R. (2016), "Restructuring of the aquatic bacterial community by hydric dynamics associated with superstorm sandy", Appl. Environ. Microbiol., 82(12), 3525-3536. https://doi.org/10.1128/AEM.00520-16
  20. Unno, T., Jang, J., Han, D., Kim, J.H., Sadowsky, M.J., Kim, O.S., Chun, J. and Hur, H.G. (2010), "Use of barcoded pyrosequencing and shared OTUS to determine sources of fecal bacteria in watersheds", Environ. Sci. Technol., 44(20), 7777-7782. https://doi.org/10.1021/es101500z
  21. United States Environmental Protection Agency (USEPA) (2012), Recreational Water Quality, USEPA, Ohio, U.S.A.