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Effect of Addition of ESCM and ESM during In Vitro Maturation on In Vitro Development of Porcine Follicular Oocytes

돼지 난포란으로부터 배반포의 체외생산에 있어서 체외성숙시 기초배양액에 ESCM과 ESM의 첨가효과

  • Kim, Seok-Gi (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Park, Hum-Dai (Department of Biotechnology, College of Engineering, Daegu University)
  • 김석기 (대구대학교 공과대학 생명공학과) ;
  • 박흠대 (대구대학교 공과대학 생명공학과)
  • Received : 2019.09.09
  • Accepted : 2019.09.23
  • Published : 2019.09.30

Abstract

In this study, we investigated the possibility of using mouse embryonic stem cell conditioned medium (ESCM) and embryonic stem cell medium (ESM) for in vitro maturation in the efficient in vitro production of blastocysts from porcine follicular oocyte. Depending on the concentration of supplement of ESCM added to the NCSU-23 solution did not affect 2-cell development rates and blastocysts development. However, in particular, the survival rate (10 days of culture) of blastocyst was significantly higher than that of the control group as the additive concentration (30%) increased (p < 0.05). The survival rate of blastocysts showed a similar tendency even with addition of ESM (30%) alone. On the other hand, the duration of the addition of these additives during IVM (0-44 h) was that the IVM I period (0-22 h) were more effective than the IVM II period (22-44 h). Thus, the effect of these additives is probably due to the combination of the various physiologically active substances of ESCM or the appropriate amino acids and vitamins of ESM. In particular, these additives were more effective during the first half (IVM I) of in vitro maturation. In summary, optimization of ESCM or ESM supplementation may improve in vitro maturation of porcine oocyte and affect developmental competency. Therefore, if more efficient methods of adding ESCM or ESM to basal culture medium can be developed during in vitro maturation of porcine follicle oocytes, high quality blastocysts will be developed from low porcine follicular oocyte compared to other domestic animals.

Keywords

References

  1. Abeydeera LR, Wang WH, Prather RS, and Day BN. 1998. Maturation in vitro of pig oocytes in protein-free culture media: Fertilization and Subsequent Embryo Development in Vitro. Biol. Reprod. 58:1316-1320. https://doi.org/10.1095/biolreprod58.5.1316
  2. Beckmann LS and Day BN. 1993. Effects of media NaCl concentration and osmolarity on the culture of early-stage porcine embryos and the viability of embryos cultured in a selected superior medium. Theriogenology 39:611-622. https://doi.org/10.1016/0093-691X(93)90248-4
  3. Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, Smuga-otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, Antosiewicz-Bourqet J, Teng JM and Tomson JA. 2011. Chemically defined conditions for human iPSC derivation and culture. Nature Methods 8(5):424-429. https://doi.org/10.1038/nmeth.1593
  4. Cheng L, Hammond H, Ye Z, Zhan X and Dravid G. 2003. Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture. Stem Cells 21(2):131-142. https://doi.org/10.1634/stemcells.21-2-131
  5. Dakhore S, Nayer B and Hasegawa K. 2018. Human Pluripotent Stem Cell Culture: Current Status, Challenges, and Advancement. Stem Cells International 2018:1-17. https://doi.org/10.1155/2018/7396905
  6. Ding J and Foxcroft GR. 1994. Epidermal growth factor enhances oocyte maturation in pigs. Mol. Reprod. Dev. 39:30-40. https://doi.org/10.1002/mrd.1080390106
  7. Ebeling S, Schuon C and Meinecke B. 2007. Mitogen activated protein kinase phosphorylation patterns in pig oocytes and cumulus cells during gonadotrophin-induced resumption of meiosis in vitro. Zygote 15(2):139-147. https://doi.org/10.1017/s0967199406004011
  8. Evans MJ and Kaufman MH. 1981. Establishment in culture of pluripotential cells from mouse embryos. Nature 292:154-156. https://doi.org/10.1038/292154a0
  9. Funahashi H and Day BN. 1993. Effects of the duration of exposure to hormone supplements on cytoplasmic maturation of pig oocytes in vitro. J. Reprod. Fertil. 98:179-185. https://doi.org/10.1530/jrf.0.0980179
  10. Hunter RHF and Greve T. 1997. Could artificial insemination of cattle be more fruitful? Penalties associated with aging eggs. Reprod. Dom. Anim. 32:137-141. https://doi.org/10.1111/j.1439-0531.1997.tb01271.x
  11. Illera MJ, Loren PL, Illera JC and Petters RM. 1998. Developmental competence of immature pig oocytes under the influence of IVM-IVF processes. Int. J. Dev. Bio. 42:1169-1172.
  12. Ivanova-Todorova E, Bochev I, Dimitrov R, Belemezova K, Mourdjeva M, Kyurkchiev S, Kinov P, Altankova I and Kyurkchiev D. 2012. Conditioned medium from adipose tissuederived mesenchymal stem cells induces CD4+FOXP3+ cells and increases IL-10 secretion. BioMed Res. Int. 2012:1-8.
  13. Khurana NK and Niemann H. 2000. Energy metabolism in preimplantation bovine embryos derived in vitro or in vivo. Biol. Reprod. 62:847-856. https://doi.org/10.1095/biolreprod62.4.847
  14. Kim EH, Cheong SA, Yoon JC, Jeon YB and Hyun SH. 2013. Optimal Derivation Timing for Establishment of Porcine Embryonic Stem Cells. J. Emb. Trans. 28:1-6. https://doi.org/10.12750/JET.2013.28.1.1
  15. Kim J, You J, Hyun SH, Lee G, Lim J and Lee E. 2010. Developmental competence of morphologically poor oocytes in relation to follicular size and oocyte diameter in the pig. Mol. Reprod. Dev. 77:330-339. https://doi.org/10.1002/mrd.21148
  16. Kishida R, Lee ES and Fukui Y. 2004. In vitro maturation of porcine oocytes using a defined medium and developmnetal capacity after intracytoplasmic sperm injection. Theriogenology 62:1663-1676. https://doi.org/10.1016/j.theriogenology.2004.03.008
  17. Kumar S, Gedam S, Biswas RK, Purkayastha A, Devi B, Bharti PK, Doley S and Kadirvel G. 2015. Effect of hormones, follicular fluid, serum and media on in vitro maturation of porcine oocyte. Indian J. Ani. Sci. 85(9):958-961.
  18. Kwon DJ, Hwang IS, Kwak TU, Oh KB, Ock SA, Chung HJ, Im GS and Hwang SS. 2015. Effect of Stem Cell-Derived Conditioned Medium on the In Vitro Maturation and Embryonic Development of Parthenogenetic Embryos in Pigs. Reprod. Dev. Biol. 39(3):89-95. https://doi.org/10.12749/RDB.2015.39.3.89
  19. Larsen WJ, Wert SE and Brunner GD. 1987. Differential modulation of rat follicle cell gap junction populartions at ovulation. Dev. Biol. 122:61-71. https://doi.org/10.1016/0012-1606(87)90332-0
  20. Lee MJ, Kim J, Lee KI, Shin JM, Chae JI and Chung HM. 2011. Enhancement of wound healing by secretory factors of endothelial precursor cells derived from human embryonic stem cells. Cytotherapy 13:165-178. https://doi.org/10.3109/14653249.2010.512632
  21. Marques MG, Nicacio AC, de Oliveira VP, Nascimento AB, Caetano HV, Mendes CM, Mello MR, Milazzotto MP, Assumpcao MEOA and Visintin JA. 2007. In vitro maturation of pig oocytes with different media, hormone and meiosis inhibitors. Ani. Reprod. Sci. 97(3-4):375-381. https://doi.org/10.1016/j.anireprosci.2006.02.013
  22. Mattioli M, Bacci ML, Galeati G and Seren E. 1991. Effect of LH and FSH on the matruation of pig oocytes in vitro. Theriogenology 36:95-105. https://doi.org/10.1016/0093-691X(91)90438-J
  23. Mattioli M, Ealeati ML and Seren E. 1989. Developmental competence of pig oocyte mature on fertilized in vitro. Theriogenology 31:1201-1207. https://doi.org/10.1016/0093-691X(89)90089-7
  24. Motlik J, Grozet N and Fulka J. 1984. Meiotic competence in vitro of pig oocytes isolated from early antral follicles. J. Reprod. Fertile. 72:323-328. https://doi.org/10.1530/jrf.0.0720323
  25. Mtango NR, Varisanga MD, Juan DY, Wongrisekeao P and Suzuki T. 2002. Development to Blastocyst Stage of Pig Oocytes Matured, Fertilized and Electroactivated In Vitro. Arch. Tierz. Dummerstorf. 6:547-556.
  26. Naito K, Fukuda Y and Toyoda Y. 1988. Effects of porcine follicular fluid on male pronucleus formation in porcine oocytes matured in vitro. Gamete. Res. 21:289-295. https://doi.org/10.1002/mrd.1120210310
  27. Neira JA, Tainturier D, Pe-a MA and Martal J. 2010. Effect of the association of IGF-I, IGF-II, bFGF, TGF- ${\beta}1$, GM-CSF, and LIF on the development of bovine embryos produced in vitro. Theriogenology 73:595-604. https://doi.org/10.1016/j.theriogenology.2009.10.015
  28. Notarianni E, Laurie S, Moor R and Evans M. 1990. Maintenance and differentiation in culture of pluripotential embryonic cell lines from pig blastocysts. J. Reprod. Fertil. 41:51-56.
  29. Papaioannou VE and Ebert KM. 1988. The preimplantation pig embryo: cell number and allocation to trophectoderm and inner cell mass of the blastocyst in vivo and in vitro. Development 102:793-803. https://doi.org/10.1242/dev.102.4.793
  30. Park CK. 1996. Factors Affecting In Vitro Maturation in Porcine Oocytes. Korean J. Emb. Trans. 11:179-191.
  31. Park YS, Kim JM and Park HD. 2004. Effects of Serum and Gonadotropins in In vitro Maturation Medium on Nuclear Maturation, Development and Cell Numbers of Korean Native Cow Embryos. Korean J. Emb. Trans. 19:229-237.
  32. Petters RM and Wells KD. 1993. Culture of pig embryos. J. Reprod. Fertil. Suppl. 48:61-73.
  33. Picton G, briggs D and Gosden R. 1998. The molecular basis of oocyte growth and development. Mol. Cell. Endocrinol. 145:27-37. https://doi.org/10.1016/S0303-7207(98)00166-X
  34. Sirard MA, Richard F, Blondin P and Robert C. 2006. Contribution of the oocyte to embryo quality. Theriogenology 65:126-136. https://doi.org/10.1016/j.theriogenology.2005.09.020
  35. Xia P, Francis R, Tekpetey FR, David T and Armstrongiy DT. 1994. Effect of IGF-I on Pig Oocyte Maturation, Fertilization, and Early Embryonic Development in Vitro, and on Granulosa and Cumulus Cell Biosynthetic Activity. Mol. Reprod. Devel. 38:373-379. https://doi.org/10.1002/mrd.1080380404
  36. Yoshida M. 1989. Improved viability of two-cell stage pig embryos resulting from in vitro fertilization of oocytes matured in vivo. J. Anim. Reprod. 35:34-37. https://doi.org/10.1262/jrd1977.35.34
  37. Yoshida M, Mizoguchi Y, Ishigaki K, Koojima T and Naga T. 1993. Birth of piglets derived from in vitro fertilization of pig oocytes matured in vitro. Theriogenology 39:1303-1311. https://doi.org/10.1016/0093-691X(93)90232-T