Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
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Current Status of Production of Transgenic Livestock by Genome Editing Technology

유전자 편집 기술에 의한 형질전환 가축의 생산 현황

  • Park, Da Som (Department of Animal Science, College of Agriculture and Life Science, Chonnam National University) ;
  • Kim, Soseob (Gwanggaeto Hanwoo) ;
  • Koo, Deog-Bon (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Kang, Man-Jong (Department of Animal Science, College of Agriculture and Life Science, Chonnam National University)
  • 박다솜 (전남대학교 농업생명과학대학 동물자원학부) ;
  • 김소섭 (광개토한우) ;
  • 구덕본 (대구대학교 공과대학 생명공학과) ;
  • 강만종 (전남대학교 농업생명과학대학 동물자원학부)
  • Received : 2019.09.11
  • Accepted : 2019.09.17
  • Published : 2019.09.30
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Abstract

The Transgenic livestock can be useful for the production of disease-resistant animals, pigs for xenotranplantation, animal bioreactor for therapeutic recombinant proteins and disease model animals. Previously, conventional methods without using artificial nuclease-dependent DNA cleavage system were used to produce such transgenic livestock, but their efficiency is known to be low. In the last decade, the development of artificial nucleases such as zinc-finger necleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regulatory interspaced short palindromic repeat (CRISPR)/Cas has led to more efficient production of knock-out and knock-in transgenic livestock. However, production of knock-in livestock is poor. In mouse, genetically modified mice are produced by coinjecting a pair of knock-in vector, which is a donor DNA, with a artificial nuclease in a pronuclear fertilized egg, but not in livestock. Gene targeting efficiency has been increased with the use of artificial nucleases, but the knock-in efficiency is still low in livestock. In many research now, somatic cell nuclear transfer (SCNT) methods used after selection of cell transfected with artificial nuclease for production of transgenic livestock. In particular, it is necessary to develop a system capable of producing transgenic livestock more efficiently by co-injection of artificial nuclease and knock-in vectors into fertilized eggs.

Keywords

References

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