한국동물생명공학회지 (Journal of Animal Reproduction and Biotechnology)

  • 제34권4호
  • /
  • Pages.318-321
  • /
  • 2019
  • /
  • 2671-4639(pISSN)
  • /
  • 2671-4663(eISSN)
한국동물생명공학회 (The Korean Society of Animal Reproduction and Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Tight Junction Assembly Ensures Maintenance of Pregnancy during Embryogenesis in a Mouse Model

  • Jeong, Yelin (Division of Animal and Dairy Sciences, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Choi, Inchul (Division of Animal and Dairy Sciences, College of Agriculture and Life Sciences, Chungnam National University)
  • 투고 : 2019.11.18
  • 심사 : 2019.11.28
  • 발행 : 2019.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Recent studies showed that tight junctions (TJs) integrity and assembly are required for blastocyst development in mouse and pig models. However, the biological functions of TJs associated with embryo implantation and maintenance of pregnancy were not investigated yet. To examine whether disrupted TJs affect further embryo development, we employed RNAi approach and inhibitor treatment. The embryos were injected with Cxadr (Coxsackievirus and adenovirus receptor) siRNA for knock down (KD) and treated with Adam10 (A Disintegrin and Metalloproteinase specific inhibitor 10; GI254023X; SI). We compared blastocyst development and paracellular sealing assay using FITC dextran uptake between control and KD or SI embryos. Finally, we transferred control and Cxadr KD or Adam 10 SI treated blastocyst to uteri of recipients. Cxadr KD and Adam 10 SI showed lower blastocyst development and more permeable to FITC-dextran. Moreover, we observed that half of KD and inhibited embryos failed to maintain pregnancies after the second trimester. Our findings suggested that TJs integrity is required for the maintenance of pregnancy and can be used as a selective marker for the successful application of assisted reproduction technologies.

키워드

참고문헌

  1. Choi I, TS Carey, CA Wilson, and JG Knott. 2012. Transcription factor AP-2gamma is a core regulator of tight junction biogenesis and cavity formation during mouse early embryogenesis. Development. 139:4623-4632. https://doi.org/10.1242/dev.086645
  2. Cockburn K, and J Rossant. 2010. Making the blastocyst: lessons from the mouse. The Journal of Clinical Investigation. 120:995-1003. https://doi.org/10.1172/JCI41229
  3. DaSilva-Arnold S, S Zamudio, A Al-Khan, and N Illsley. 2016. The tight junction protein occludin is regulated by RhoA and activin A in JEG3 trophoblast cells. Placenta. 45:116.
  4. Jeong Y, S-A Ock, JG Yoo, D-Y Yu, and I Choi. 2019. The Cxadr- Adam10 complex plays pivotal roles in tight junction integrity and early trophoblast development in mice. Molecular Reproduction and Development. https://doi.org/10.1002/ mrd.23250.
  5. Kulandavelu S, D Qu, N Sunn, J Mu, MY Rennie, KJ Whiteley, JR Walls, NA Bock, JCH Sun, A Covelli, JG Sled, and SL Adamson. 2006. Embryonic and Neonatal Phenotyping of Genetically Engineered Mice. ILAR Journal. 47:103-117. https://doi.org/10.1093/ilar.47.2.103
  6. Kwon J, N-H Kim, and I Choi. 2016b. CXADR is required for AJ and TJ assembly during porcine blastocyst formation. Reproduction. 151:297-304. https://doi.org/10.1530/REP-15-0397
  7. Kwon J, S Jeong, I Choi, and NH Kim. 2016a. ADAM10 Is Involved in Cell Junction Assembly in Early Porcine Embryo Development. PLoS One. 11:e0152921. https://doi.org/10.1371/journal.pone.0152921
  8. McLaren A, and R Smith. 1977. Functional test of tight junctions in the mouse blastocyst. Nature. 267:351. https://doi.org/10.1038/267351a0
  9. Mobley RJ, D Raghu, LD Duke, K Abell-Hart, JS Zawistowski, K Lutz, SM Gomez, S Roy, R Homayouni, GL Johnson, and AN Abell. 2017. MAP3K4 Controls the Chromatin Modifier HDAC6 during Trophoblast Stem Cell Epithelial-to-Mesenchymal Transition. Cell Reports. 18:2387-2400. https://doi.org/10.1016/j.celrep.2017.02.030
  10. Sivasubramaniyam T, J Garcia, A Tagliaferro, M Melland-Smith, S Chauvin, M Post, T Todros, and I Caniggia. 2013. Where Polarity Meets Fusion: Role of Par6 in Trophoblast Differentiation during Placental Development and Preeclampsia. Endocrinology. 154:1296-1309. https://doi.org/10.1210/en.2012-1823
  11. Watson AJ, and LC Barcroft. 2001. Regulation of blastocyst formation. Front Biosci. 6:D708-730. https://doi.org/10.2741/Watson