Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Prevalence and Diversity of MBL Gene-Containing Integrons in Metallo-β-Lactamase (MBL)-Producing Pseudomonas spp. Isolates Disseminated in a Korean Hospital

  • Yum, Jong Hwa (Department of Clinical Laboratory Science, Dongeui University)
  • Received : 2019.08.26
  • Accepted : 2019.10.14
  • Published : 2019.12.31
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Abstract

Carbapenem is recently considered as the last resort of the therapeutics for gram negative bacterial infection. Increasing of organisms producing metallo-β-lactamase (MBL), we have difficulty in choosing the antimicrobial agents. Among 345 clinical isolates of Pseudomonas spp., 61 isolates (17.7%) were positive for the modified imipenem or meropenem-Hodge test and 55 isolates (15.9%) were positive for the imipenem-EDTA + SMA double disk synergy test (DDS). PCR and sequencing of blaVIM-2-allele and blaIMP-1-allele showed that 17 isolates of Pseudomonas aeruginosa, 9 isolates of Pseudomonas taiwnensis and 2 Pseudomonas plecoglossicida had blaVIM-2, and 22 isolates of P. aeruginosa and one Pseudomonas otitidis had blaIMP-6. These MBL genes were all in class 1 integron. The size of class 1 integron with blaVIM-2 ranged from 3.5 kb to 5.5 kb in clinical isolates of Pseudomonas spp. including P. aeruginosa. blaVIM-2 was most often located first in the class 1 integron, sometimes in the second or third position, and these integrons often had aacA4 or aadA1. Strict infection control measures are needed to more effectively prevent further spread of these MBL-producing Pseudomonas spp. In addition, MBL-producing Pseudomonas spp. is expected to continue to spread in various countries and regions.

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References

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