Asian-Australasian Journal of Animal Sciences

  • 제32권1호
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  • Pages.103-109
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  • 2019
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Evaluation of in vitro ruminal fermentation of ensiled fruit byproducts and their potential for feed use

  • Mousa, Shimaa A (Graduate School of Science and Technology, Shinshu University) ;
  • Malik, Pradeep K. (Energy Metabolism Laboratory, ICAR-National Institute of Animal Nutrition and Physiology) ;
  • Kolte, Atul P. (Energy Metabolism Laboratory, ICAR-National Institute of Animal Nutrition and Physiology) ;
  • Bhatta, Raghavendra (Energy Metabolism Laboratory, ICAR-National Institute of Animal Nutrition and Physiology) ;
  • Kasuga, Shigemitsu (Graduate School of Science and Technology, Shinshu University) ;
  • Uyeno, Yutaka (Graduate School of Science and Technology, Shinshu University)
  • 투고 : 2018.04.12
  • 심사 : 2018.05.24
  • 발행 : 2019.01.01
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초록

Objective: Ensiling of tannin-rich fruit byproducts (FB) involves quantitative and qualitative changes in the tannins, which would consequently change the rumen fermentation characteristics. This study aimed to evaluate whether ensiled FBs are effective in mitigating methane emission from ruminants by conducting in vitro assessments. Methods: Fruit byproducts (grape pomace, wild grape pomace, and persimmon skin) were collected and subjected to four-week ensiling by Lactobacillus buchneri inoculant. A defined feed component with or without FB samples (both fresh and ensiled material) were subjected to in vitro anaerobic culturing using rumen fluid sampled from beef cattle, and the fermentation parameters and microbial populations were monitored. Results: Reduced methane production and a proportional change in total volatile fatty acids (especially enhanced propionate proportion) was noted in bottles containing the FBs compared with that in the control (without FB). In addition, we found lower gene copy number of archaeal 16S rRNA and considerably higher levels of one of the major fibrolytic bacteria (Fibrobacter succinogenes) in the bottles containing FBs than in the control, particularly, when it was included in a forage-based feed. However, in the following cultivation experiment, we observed that FBs failed to exhibit a significant difference in methane production with or without polyethylene glycol, implying that tannins in the FBs may not be responsible for the mitigation of methane generation. Conclusion: The results of the in vitro cultivation experiments indicated that not only the composition but also ensiling of FBs affected rumen fermentation patterns and the degree of methane generation. This is primarily because of the compositional changes in the fibrous fraction during ensiling as well as the presence of readily fermented substrates, whereas tannins in these FBs seemed to have little effect on the ruminal fermentation kinetics.

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참고문헌

  1. Baumgartel T, Kluth H, Epperlein K, Rodehutscord M. A note on digestibility and energy value for sheep of different grape pomace. Small Rumin Res 2007;67:302-6. https://doi.org/10.1016/j.smallrumres.2005.11.002
  2. Abarghuei MJ, Rouzbehan Y, Alipour D. The influence of the grape pomace on the ruminal parameters of sheep. Livest Sci 2010;132:73-9. https://doi.org/10.1016/j.livsci.2010.05.002
  3. Kim HY, Song YM, Jin SK, et al. The effect of change in meat quality parameters on pig Longissimus dorsi muscle by the addition of fermented persimmon shell diet. Asian-Australas J Anim Sci 2006;19:286-91. https://doi.org/10.5713/ajas.2006.286
  4. Kim HY, Song YM, Kang YS, et al. The effect of fermented persimmon shell diet supplementation on the growth performance and blood parameters in finishing pigs. Anim Sci J 2006;77:314-9. https://doi.org/10.1111/j.1740-0929.2006.00354.x
  5. Hristov AN, Oh J, Giallongo F, et al. An inhibitor persistently decreased enteric methane emission from dairy cows with no negative effect on milk production. Proc Natl Acad Sci USA 2015;112:10663-8. https://doi.org/10.1073/pnas.1504124112
  6. Martin C, Morgavi DP, Doreau M. Methane mitigation in ruminants: from microbe to the farm scale. Animal 2010;4:351-65. https://doi.org/10.1017/S1751731109990620
  7. Uyeno Y. Selective inhibition of harmful rumen microbes. In: Puniya AK, Singh R, Kamra DN, editors. Rumen microbiology: From evolution to revolution. Berlin, Germany: Springer-Verlag GmbH; 2015. p. 199-211.
  8. AOAC (Association of Official Analytical Chemists). In: AOAC. editor. Official methods of analysis, 15th Ed. Arlington, VA, USA: AOAC International; 1990.
  9. Uyeno Y, Konaka R, Shirota M, Kobayashi S. Ensiling fruit byproducts with inoculum of lactic acid bacteria strains. Anim Nutr Feed Technol 2016;16:515-9. https://doi.org/10.5958/0974-181X.2016.00041.X
  10. Alipour D, Rouzbehan Y. Effects of ensiling grape pomace and addition of polyethylene glycol on in vitro gas production and microbial biomass yield. Anim Feed Sci Technol 2007;137:138-49. https://doi.org/10.1016/j.anifeedsci.2006.09.020
  11. NRC. Nutrient requirements of dairy cattle. 7th ed. Washington, DC, USA: National Academy Press; 2001.
  12. Makkar HP. Quantification of tannins in tree and shrub foliage: a laboratory manual. Dordrecht, Netherlands: Springer; 2003.
  13. Hiramori C, Uyeno Y, Koh K. Effects of lactic acid bacteria on fermented apple pomace (FAP) production and the inclusion of an FAP into a medium for bunashimeji (Hypsizygus marmoreus) cultivation. Mushroom Sci Biotechnol 2015;23:120-4.
  14. Uyeno Y, Wang C, Jayanegara A, et al. Increase in rumen fibrolytic bacteria and the improvement of fiber degradability of ensiling total mixed ration assessed by in vitro rumen culture. Adv Anim Vet Sci 2016;4:183-6. https://doi.org/10.14737/journal.aavs/2016/4.4.183.186
  15. Bhatta R, Uyeno Y, Tajima K, et al. Difference in the nature of tannins on in vitro ruminal methane and volatile fatty acid production and on methanogenic archaea and protozoal populations. J Dairy Sci 2009;92:5512-22. https://doi.org/10.3168/jds.2008-1441
  16. Abrar A, Kondo M, Kitamura T, Ban-Tokuda T, Matsui H. Effect of supplementation of rice bran and fumarate alone or in combination on in vitro rumen fermentation, methanogenesis and methanogens. Anim Sci J 2016;87:398-404. https://doi.org/10.1111/asj.12431
  17. Denman SE, Tomkins NW, McSweeney CS. Quantitation and diversity analysis of ruminal methanogenic populations in response to the antimethanogenic compound bromochloromethane. FEMS Microbiol Ecol 2007;62:313-22. https://doi.org/10.1111/j.1574-6941.2007.00394.x
  18. Lettat A, Hassanat F, Benchaar C. Corn silage in dairy cow diets to reduce ruminal methanogenesis: effects on the rumen metabolically active microbial communities. J Dairy Sci 2013;96: 5237-48. https://doi.org/10.3168/jds.2012-6481
  19. Stevenson D, Weimer P. Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR. Appl Microbiol Biotechnol 2007;75:165-74. https://doi.org/10.1007/s00253-006-0802-y
  20. Tabacco E, Righi F, Quarantelli A, Borreani G. Dry matter and nutritional losses during aerobic deterioration of corn and sorghum silages as influenced by different lactic acid bacteria inocula. J Dairy Sci 2011;94:1409-19. https://doi.org/10.3168/jds.2010-3538
  21. Nishino N, Wada H, Yoshida M, Shiota H. Microbial counts, fermentation products, and aerobic stability of whole crop corn and a total mixed ration ensiled with and without inoculation of Lactobacillus casei or Lactobacillus buchneri. J Dairy Sci 2004; 87:2563-70. https://doi.org/10.3168/jds.S0022-0302(04)73381-0
  22. Goeser J, Heuer C, Crump P. Forage fermentation product measures are related to dry matter loss through meta-analysis. Prof Anim Sci 2015;31:137-45. https://doi.org/10.15232/pas.2014-01356
  23. Kondo M, Jayanegara A, Uyeno Y, Matsui H. Variation of tannin contents in selected agro-industrial by-products and their biological activity in precipitating protein. Adv Anim Vet Sci 2016; 4:66-70. https://doi.org/10.14737/journal.aavs/2016/4.2.66.70
  24. Patra AK, Saxena J. Exploitation of dietary tannins to improve rumen metabolism and ruminant nutrition. J Sci Food Agric 2011;91:24-37. https://doi.org/10.1002/jsfa.4152
  25. Malik P, Kolte A, Baruah L, et al. Enteric methane mitigation in sheep through leaves of selected tanniniferous tropical tree species. Livest Sci 2017;200:29-34. https://doi.org/10.1016/j.livsci.2017.04.001
  26. Dehority BA. Numbers, factors affecting the population and distribution of rumen bacteria. In: Dehority BA, editor. Rumen microbiology. Nottingham, UK: Nottingham University Press; 2003. p. 265-94.
  27. Cao Y, Takahashi T, Horiguchi K-i, Yoshida N, Cai Y. Methane emissions from sheep fed fermented or non-fermented total mixed ration containing whole-crop rice and rice bran. Anim Feed Sci Technol 2010;157:72-8. https://doi.org/10.1016/j.anifeedsci.2010.02.004
  28. Cao Y, Takahashi T, Horiguchi Ki, Yoshida N, Zhou D. In vitro ruminal dry matter digestibility and methane production of fermented total mixed ration containing whole-crop rice and rice bran. Grassl Sci 2012;58:133-9. https://doi.org/10.1111/j.1744-697X.2012.00254.x
  29. Wang C, Uyeno Y, Jayanegara A, et al. Changes in in vitro rumen fermentation characteristics of different compositions of total mixed rations (TMR) and the ensiled TMRs. Adv Anim Vet Sci 2016;4:179-82.
  30. Jayanegara A, Togtokhbayar N, Makkar HP, Becker K. Tannins determined by various methods as predictors of methane production reduction potential of plants by an in vitro rumen fermentation system. Anim Feed Sci Technol 2009;150:230-7. https://doi.org/10.1016/j.anifeedsci.2008.10.011

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