Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of zinc-bearing palygorskite on rumen fermentation in vitro

  • Chen, Mengjiao (Institute of Dairy Science, College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Xi, Yumeng (Animal Husbandry Institute, Jiangsu Academy of Agricultural Sciences) ;
  • Zhang, Lin (Institute of Dairy Science, College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Zeng, Hanfang (Institute of Dairy Science, College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Li, Yeqing (Institute of Dairy Science, College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Han, Zhaoyu (Institute of Dairy Science, College of Animal Science and Technology, Nanjing Agricultural University)
  • Received : 2017.12.21
  • Accepted : 2018.04.16
  • Published : 2019.01.01
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Abstract

Objective: The aim of the study was to investigate the effect of zinc-bearing palygorskite (Zn-Pal) on rumen fermentation by in vitro gas-production system. Methods: In trial, 90 incubators were evenly divided into five groups: control (0% Zn-Pal), treatment I (0.2% Zn-Pal), treatment II (0.4% Zn-Pal), treatment III (0.6% Zn-Pal), and treatment IV (0.8% Zn-Pal). The contents of zinc for treatments were 0, 49, 98, 147, 196 mg/kg, respectively. The main chemical composition and microstructure of Zn-Pal was investigated by X-ray diffraction. The physicochemical features were evaluated by Zeta potential analysis, cation-exchange capacity, ethylene blue absorption and specific surface area (the Brunauer-Emmett-Teller method). In vitro gas production (GP) was recorded at 3, 6, 9, 12, 18, 24, 36, 48, 60, and 72 h incubation. Incubation was stopped at 0, 6, 12, 24, 48, and 72 h and the inoculants were tested for pH, microbial protein yield (MCP), $NH_3-N$, volatile fatty acids (VFAs), lipopolysaccharide (LPS). Results: The results showed that the GP in the treatment groups was not significantly different from the control groups (p>0.05). Compared to the control group, pH was higher at 24 h, 48 h (p<0.05), and 72 h (p<0.01) (range 6 to 7). The concentration of $NH_3-N$ in the three treatment groups was higher than in the control group at 24 h (p<0.01), meanwhile, it was lower at 48 h and 72 h (p<0.01), except in the treatment IV. The concentration of MCP in treatment I group was higher than in the control at 48 h (p<0.01). Compared with control, the LPS concentration in treatment III became lower at 12 h (p<0.05). Total VFAs in treatments were higher than in the control at 24 h, 48 h (p<0.05). Conclusion: These results suggest that the addition of Zn-Pal can improve the rumen fermentation, especially when adding 0.2% Zn-Pal.

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References

  1. Xu J, Wang W, Mu B, Wang A. Effects of inorganic sulfates on the microstructure and properties of ion-exchange treated palygorskite clay. Colloids Surf A Physicochem Eng Asp 2012; 405:59-64. https://doi.org/10.1016/j.colsurfa.2012.04.037
  2. Chalvatzi S, Kalamaki MS, Arsenos G, Fortomaris P. Dietary supplementation with the clay mineral palygorskite affects performance and beneficially modulates cecal microbiota in laying pullets. J Appl Microbiol 2016;120:1033-40. https://doi.org/10.1111/jam.13041
  3. Reynolds JEF. Martindale: the extra pharmacopoeia. 28th ed. London, UK: Royal Pharmaceutical Society; 1982.
  4. Zaid MR, Hasan M, Khan AA. Attapulgite in the treatment of acute diarrhoea: a double-blind placebo-controlled study. J Diarrhoeal Dis Res 1995;13:44-6.
  5. Zhang J, Lv Y, Tang C, Wang X. Effects of dietary supplementation with palygorskite on intestinal integrity in weaned piglets. Appl Clay Sci 2013;86:185-9. https://doi.org/10.1016/j.clay.2013.10.009
  6. Fischer Walker C, Black RE. Zinc and the risk for infectious disease. Annu Rev Nutr 2004;24:255-75. https://doi.org/10.1146/annurev.nutr.23.011702.073054
  7. Kennedy DW, Bunting LD. Alterations in ruminal utilization of magnesium and zinc in lambs fed different ratios of concentrate: forage. Int J Vitam Nutr Res 1991;61:67-71.
  8. Wang LC, Zhang TT, Wen C, et al. Protective effects of zinc-bearing clinoptilolite on broilers challenged with Salmonella pullorum. Poult Sci 2012;91:1838-45. https://doi.org/10.3382/ps.2012-02284
  9. Hu CH, Qian ZC, Song J, Luan ZS, Zuo AY. Effects of zinc oxide-montmorillonite hybrid on growth performance, intestinal structure, and function of broiler chicken. Poult Sci 2013; 92:143-50. https://doi.org/10.3382/ps.2012-02250
  10. Tang ZG, Wen C, Wang LC, Wang T, Zhou YM. Effect of zinc-bearing zeolite clinoptilolite on growth performance, nutrient retention, digestive enzyme activities, and intestinal function of broiler chickens. Biol Trace Elem Res 2014;158:51-7. https://doi.org/10.1007/s12011-014-9900-3
  11. Yan R, Zhang L, Yang X, Wen C, Zhou Y. Bioavailability evaluation of zinc-bearing palygorskite as a zinc source for broiler chickens. Appl Clay Sci 2016;119:155-60. https://doi.org/10.1016/j.clay.2015.07.027
  12. Qiao L, Chen Y, Wen C, Zhou Y. Effects of natural and heat modified palygorskite supplementation on the laying performance, egg quality, intestinal morphology, digestive enzyme activity and pancreatic enzyme mRNA expression of laying hens. Appl Clay Sci 2015;104:303-8. https://doi.org/10.1016/j.clay.2014.12.010
  13. Theodorou MK, Williams BA, Dhanoa MS, Mcallan AB, France J. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Anim Feed Sci Technol 1994;48:185-97. https://doi.org/10.1016/0377-8401(94)90171-6
  14. Mcdonald I. A revised model for the estimation of protein degradability in the rumen. J Agric Sci 1981;96:251-2. https://doi.org/10.1017/S0021859600032081
  15. Makkar HP, Sharma OP, Dawra RK, et al. Simple determination of microbial protein in rumen liquor. J Dairy Sci 1982;65: 2170-3. https://doi.org/10.3168/jds.S0022-0302(82)82477-6
  16. Emmanuel DGV, Dunn SM, Ametaj BN. Feeding high proportions of barley grain stimulates an inflammatory response in dairy cows. J Dairy Sci 2008;91:606-14. https://doi.org/10.3168/jds.2007-0256
  17. Wang W, Li A, Zhang J, Wang A. Study on superabsorbent composite. XI. Effect of thermal treatment and acid activation of attapulgite on water absorbency of poly (-acrylic acid)/attapulgite superabsorbent composite. Polym Compos 2007; 28:397-404. https://doi.org/10.1002/pc.20299
  18. Marchuk A, Rengasamy P. Clay behaviour in suspension is related to the ionicity of clay-cation bonds. Appl Clay Sci 2011;53:754-9. https://doi.org/10.1016/j.clay.2011.05.019
  19. Hamieh T, Siffert B. Theoretical and experimental study of the surface charge density and the surface potential of coal-water suspensions in dissymmetrical electrolytes. Colloids Surf A Physicochem Eng Asp 1994;84:217-28. https://doi.org/10.1016/0927-7757(93)02723-R
  20. Rusmin R, Sarkar B, Biswas B, et al. Structural, electrokinetic and surface properties of activated palygorskite for environmental application. Appl Clay Sci 2016;134:95-102. https://doi.org/10.1016/j.clay.2016.07.012
  21. Kang S, Wanapat M. Using plant source as a buffering agent to manipulating rumen fermentation in an in vitro gas production system. Asian-Australas J Anim Sci 2013;26:1424-36. https://doi.org/10.5713/ajas.2013.13153
  22. Ehrlich WK, Davison TM. Adding bentonite to sorghum grain-based supplements has no effect on cow milk production. Aust J Exp Agric 1997;37:505-8. https://doi.org/10.1071/EA96140
  23. Martinez ME, Ranilla MJ, Tejido ML, Ramos S, Carro MD. Comparison of fermentation of diets of variable composition and microbial populations in the rumen of sheep and Rusitec fermenters. I. Digestibility, fermentation parameters, and microbial growth. J Dairy Sci 2010;93:3684-98. https://doi.org/10.3168/jds.2009-2933
  24. Kardaya D, Sudrajat D, Dihansih E. Efficacy of dietary urea-impregnated zeolite in improving rumen fermentation characteristics of local lamb. Media Peternakan 2012;35:207-13. https://doi.org/10.5398/medpet.2012.35.3.207
  25. Zeng HF, Lin LJ, Xi YM, Han ZY. Effects of raw and heated palygorskite on rumen fermentation in vitro. Appl Clay Sci 2017;138:125-30. https://doi.org/10.1016/j.clay.2017.01.006
  26. Bampidis VA, Christodoulou V, Theophilou N, Kotsampasi V. Effect of dietary palygorskite on performance and blood parameters of lactating Holstein cows. Appl Clay Sci 2014; 91-92:25-9. https://doi.org/10.1016/j.clay.2014.02.006
  27. Grabherr H, Spolders M, Furll M, et al. Effect of several doses of zeolite A on feed intake, energy metabolism and on mineral metabolism in dairy cows around calving. J Anim Physiol Anim Nutr 2009;93:221-36. https://doi.org/10.1111/j.1439-0396.2008.00808.x
  28. Van SPJ, Fadel J, Sniffen CJ. Discount factors for energy and protein in ruminant feeds [Formulation of diets]. In Proceedings Cornell Nutrition Conference for Feed Manufacturers; 1979.
  29. Salem FA, Soliman AS, Abdelmawla SM, Elmahdy MRM. Effect of some feed additives to rations of growing sheep on growing performance, rumen fermentation, blood constituents and carcass characteristics. Annu Agric Sci Mosh 2000;38: 1885-904.
  30. Bento CB, Azevedo AC, Gomes DI, et al. Effect of protein supplementation on ruminal parameters and microbial community fingerprint of Nellore steers fed tropical forages. Animal 2016;10:44-54. https://doi.org/10.1017/S1751731115001512
  31. Plaizier JC, Krause DO, Gozho GN, Mcbride BW. Subacute ruminal acidosis in dairy cows: the physiological causes, incidence and consequences. Vet J 2008;176:21-31. https://doi.org/10.1016/j.tvjl.2007.12.016
  32. Bun SD, Guo YM, Guo FC, Ji FJ, Cao H. Influence of organic zinc supplementation on the antioxidant status and immune responses of broilers challenged with eimeria tenella. Poult Sci 2011;90:1220-6. https://doi.org/10.3382/ps.2010-01308
  33. Sanchezsalcedo S, Shruti S, Salinas AJ, et al. In vitro antibacterial capacity and cytocompatibility of $SiO_2-CaO-P_2O_5$ meso-macro porous glass scaffolds enriched with ZnO. J Mater Chem B 2014;2:4836-47. https://doi.org/10.1039/C4TB00403E
  34. Abend S, Lagaly G. Sol-gel transitions of sodium montmorllonite dispersions. Aappl Clay Sci 2000;131-5.
  35. Zhou J, Dong G, Ao C, et al. Feeding a high-concentrate corn straw diet increased the release of endotoxin in the rumen and pro-inflammatory cytokines in the mammary gland of dairy cows. BMC Vet Res 2014;10:172. https://doi.org/10.1186/s12917-014-0172-0

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