Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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The Anaesthetic Effect of MS-222 for Longtooth Grouper, Epinephelus moara and the Hybrid Grouper, E. moara ♀ × E. lanceolatus ♂

자바리(Epinephelus moara)와 대왕자바리(E. moara ♀ × E. lanceolatus ♂)에 대한 MS-222의 마취효과

  • Park, Jong Yeon (Department of Life Science & Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Kim, Kang-Rae (Department of Life Science & Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Bang, In-Chul (Department of Life Science & Biotechnology, College of Natural Sciences, Soonchunhyang University)
  • 박종연 (순천향대학교 생명시스템학과) ;
  • 김강래 (순천향대학교 생명시스템학과) ;
  • 방인철 (순천향대학교 생명시스템학과)
  • Received : 2019.10.11
  • Accepted : 2019.10.31
  • Published : 2019.12.31
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Abstract

The anaesthetic effect of tricaine methanesulfonate (MS-222) concentrations and water temperatures for longtooth grouper (Epinephelus moara) and hybrid grouper (E. moara ♀ × E. lanceolatus ♂) were investigated. Anesthetic induction and recovery time were measured at 18, 22, 26 and 30℃ of Cwater temperature and 100, 150, 200 and 250 ppm of anesthetic concentrations. Anesthetic induction time tended to decrease with increasing concentration and water temperature. Recovery time was proportional to concentration, but inversely proportional to water temperature. However, there was no significant differences in recovery time at 22℃ or lower. The optimal anesthesia condition was 30℃ and 100 ppm, which was the shortest recovery time for longtooth grouper, and 150 ppm at 30℃ in the case of hybrid grouper because anesthetic time is significantly different with 100 ppm in spite of no significant differences with 100 ppm for recovery time. As a results of two-way ANOVA test, there was a significant difference between the species of longtooth and hybrid grouper. On the other hand, there was no interaction effect between concentration and species. Also, there was no interaction effect among species, concentration, and water temperature.

자바리와 대왕자바리 2종을 대상으로 MS-222 농도 및 수온에 따른 마취효과 실험을 수행하였다. 농도는 100, 150, 200 그리고 250 ppm을, 수온은 18, 22, 26 그리고 30℃에서 마취 및 회복시간을 조사하였다. MS-222 농도와 수온이 높아질수록 마취시간이 짧아지는 경향을 보였으며, 회복시간은 농도와는 비례하지만 수온과는 반비례하는 경향을 보였다. 회복시간은 22℃ 이하에서는 큰 차이가 없었다. 자바리의 최적 마취조건은 회복시간이 가장 짧은 30℃와 100 ppm, 대왕자바리의 경우 30℃ 150 ppm이 회복시간이 100 ppm과 유의적으로 차이가 없으면서 마취시간은 유의하게 짧아 가장 적합했다. 이원배치분산분석 결과 자바리와 대왕자바리의 종 간 유의한 차이가 있었으나, 종과 농도 그리고 종, 농도, 수온에 대한 상호작용 효과는 없었다.

Keywords

References

  1. Chen, Z.F., Y.S. Tian, P.F. Wang, J. Tang, J.C. Liu, W.H. Ma and J.M. Zhai. 2018. Embryonic and larval development of a hybrid between kelp grouper Epinephelus moara $female{\times}giant$ grouper E. lanceolatus male using cryopreserved sperm. Aquac. Res., 49: 1407-1413. https://doi.org/10.1111/are.13591
  2. Han, S.J., K.M. Kim, N.J. Choi, J.H. Koo, C.K. Park, W.G. Lee and S.C. Ji. 2011. Comparative efficacy of clove oil, MS-222 and 2-Phenoxyethanol as anesthetics in juvenile Scomber japonicus. Kor. J. Fish Aquat. Sci., 44: 653-657. (in Korean) https://doi.org/10.5657/KFAS.2011.0653
  3. Houston, A.H., C.L. Czerwinski and R.J. Woods. 1973. Cardiovascular and respiratory activity during recovery from anaesthesia and surgery in brook trout (Salvelinus fontinalis) and carp (Cyprinus carpio). J. Fish. Res. Board Can., 30: 1705-1712. https://doi.org/10.1139/f73-270
  4. Kaschner, K., K. Kesner-Reyes, C. Garilao, J. Rius-Barile, T. Rees and R. Froese. 2016. AquaMaps: predicted range maps for aquatic species. World wide web electronic publication. www.aquamaps.org (version 08/2016)
  5. Lee, H.B., H.S. Jung, G.M. Ko and D.S. Kim. 2017. Comparative efficacy of 5 anesthetic agents in the glass catfish Kryptopterus vitreolus. Kor. J. Fish Aquat. Sci., 50: 824-828. (in Korean) https://doi.org/10.5657/KFAS.2017.0824
  6. Malmstrom, T., R. Salte, H.M. Gjoeen and A Linseth. 1993. A practical evaluation of metomidate and MS-222 as anaesthetics for Atlantic halibut (Hippoglossus hippoglossus L.). Aquaculture, 113: 331-338. https://doi.org/10.1016/0044-8486(93)90403-L
  7. Marking, L.L. 1967. Toxicity of MS-222 to selected fishes. 9pp. U.S. Fish and Wildlife Services, Investigations into Fish Control 12.
  8. Marking, L.L. and F.P. Meyer. 1985. Are better anesthetics needed in fisheries?. Fisheries, 10: 2-5. https://doi.org/10.1577/1548-8446(1985)010<0002:ABANIF>2.0.CO;2
  9. Massee, K.C., M.B. Rust, R.W. Hardy and R.S. Stickney. 1995. The effectiveness of tricaine, quinaldine sulfate and metomidate as anesthetics for larval fish. Aquaculture, 134: 315-359.
  10. Min, O.P., W.J. Hur, S.Y. Im, D.W. Seol, J. Lee and I.S. Park. 2008. Anaesthetic efficacy and physiological responses to clove oil-anaesthetized kelp grouper Epinephelus bruneus. Aquac. Res., 39: 877-884. https://doi.org/10.1111/j.1365-2109.2008.01941.x
  11. Murai, T. and M.R. Catacutan. 1981. Effect of 2-phenoxy ethanol and MS-222 on milkfish fingerlings, Chanos chanos as anesthetic agents. SEAFDEC Aquaculture Department Quarterly Research Report, 5: 19-.21.
  12. Park, I.S., J.M. Kim, Y.H. Kim. and D.S. Kim. 1988. Influence of lidocaine as an anaesthetic for marine fishes. J. Fish. Pathol., 1: 123-130. (in Korean)
  13. Park, J.Y. 2016. Effect of salinity change on stress response of longtooth grouper (Epinephelus bruneus) and longtooth giant grouper (E. bruneus $female{\times}E$. lanceolatus male). Master's thesis Mokpo National University, Mokpo, 27pp. (in Korean)
  14. Pirhonen, J. and C.B. Schreck. 2003. Effects of anaesthesia with MS-222, clove oil and $CO_2$ on feed intake and plasma cortisol in steelhead trout (Oncorchynchus mykiss). Aquaculture, 220: 507-514. https://doi.org/10.1016/S0044-8486(02)00624-5
  15. Pramod, P.K., T.P. Sajeevan, A. Ramachandran, S. Thampy and S.S. Pai. 2010. Effects of two anesthetics on water quality during simulated transport of a tropical ornamental fish, the Indian tiger barb Puntius filamentosus. North American Journal of Aquaculture, 72: 290-297. https://doi.org/10.1577/A09-063.1
  16. Sada, E.K. 1985. Influence of the anesthetic quinaldine on the some tilapias. Aquaculture, 46: 55-62. https://doi.org/10.1016/0044-8486(85)90175-9
  17. Son, M.H., M.W. Park, J.I. Myeong, D.J. Kim, B.H. Kim, Q. Jo and I.G. Jeon. 2001. Anaesthetic tolerance of juvenile black rockfish Sebastess schlegeli produced for wild stock enhancement. Ocean Polar Res., 23: 285-290. https://doi.org/10.1016/S0141-1187(01)00018-9
  18. Son, M.H. and H.K. Lim. 2008. Comparison of anesthetic tolerance between the wild and cultured fish, black seabream Acanthopagrus schlegeli juvenile. J. Aquaculture, 21: 304-308. (in Korean)
  19. Song, Y.B., S.R. Oh, J.P. Seo, B.G. Ji, B.S. Lim and Y.D. Lee. 2005. Larval development and rearing of longthooth grouper Epinephelus bruneus in Jeju island, Korea. J. World Aquacul. Soc., 36: 209-216. https://doi.org/10.1111/j.1749-7345.2005.tb00387.x
  20. Tang, J., Y. Tian, Z. Li, M. Cheng, Z. Chen, D. Mao and B. Li. 2018. Analysis of genetic characters in Epinephelus moara, E. lanceolaus and their hybrids. J. Agricul. Biotech., 26: 819-829.
  21. Topic Popovic, N., I. Strunjak-Perovic, R. Coz-Rakovac, J. Barisic, M. Jadan, A. Persin Berakovic and R. Sauerborn Klobucar. 2012. Tricaine methane-sulfonate (MS-222) application in fish anaesthesia. J. Appl. Ichthyol., 28: 553-564. https://doi.org/10.1111/j.1439-0426.2012.01950.x
  22. Weber, R.A., J.B. Peleterio, L.O. Garcia Marin and M. Aldegunde. 2009. The efficacy of 2-phenoxyethanol, metomidate, clove oil and MS-222 as anesthetic agents in the Senegalese sole (Solea senegalensis Kaup 1858). Aquaculture, 288: 147-150. https://doi.org/10.1016/j.aquaculture.2008.11.024