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자바리(Epinephelus moara)와 대왕자바리(E. moara ♀ × E. lanceolatus ♂)에 대한 MS-222의 마취효과

The Anaesthetic Effect of MS-222 for Longtooth Grouper, Epinephelus moara and the Hybrid Grouper, E. moara ♀ × E. lanceolatus ♂

  • 박종연 (순천향대학교 생명시스템학과) ;
  • 김강래 (순천향대학교 생명시스템학과) ;
  • 방인철 (순천향대학교 생명시스템학과)
  • Park, Jong Yeon (Department of Life Science & Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Kim, Kang-Rae (Department of Life Science & Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Bang, In-Chul (Department of Life Science & Biotechnology, College of Natural Sciences, Soonchunhyang University)
  • 투고 : 2019.10.11
  • 심사 : 2019.10.31
  • 발행 : 2019.12.31

초록

자바리와 대왕자바리 2종을 대상으로 MS-222 농도 및 수온에 따른 마취효과 실험을 수행하였다. 농도는 100, 150, 200 그리고 250 ppm을, 수온은 18, 22, 26 그리고 30℃에서 마취 및 회복시간을 조사하였다. MS-222 농도와 수온이 높아질수록 마취시간이 짧아지는 경향을 보였으며, 회복시간은 농도와는 비례하지만 수온과는 반비례하는 경향을 보였다. 회복시간은 22℃ 이하에서는 큰 차이가 없었다. 자바리의 최적 마취조건은 회복시간이 가장 짧은 30℃와 100 ppm, 대왕자바리의 경우 30℃ 150 ppm이 회복시간이 100 ppm과 유의적으로 차이가 없으면서 마취시간은 유의하게 짧아 가장 적합했다. 이원배치분산분석 결과 자바리와 대왕자바리의 종 간 유의한 차이가 있었으나, 종과 농도 그리고 종, 농도, 수온에 대한 상호작용 효과는 없었다.

The anaesthetic effect of tricaine methanesulfonate (MS-222) concentrations and water temperatures for longtooth grouper (Epinephelus moara) and hybrid grouper (E. moara ♀ × E. lanceolatus ♂) were investigated. Anesthetic induction and recovery time were measured at 18, 22, 26 and 30℃ of Cwater temperature and 100, 150, 200 and 250 ppm of anesthetic concentrations. Anesthetic induction time tended to decrease with increasing concentration and water temperature. Recovery time was proportional to concentration, but inversely proportional to water temperature. However, there was no significant differences in recovery time at 22℃ or lower. The optimal anesthesia condition was 30℃ and 100 ppm, which was the shortest recovery time for longtooth grouper, and 150 ppm at 30℃ in the case of hybrid grouper because anesthetic time is significantly different with 100 ppm in spite of no significant differences with 100 ppm for recovery time. As a results of two-way ANOVA test, there was a significant difference between the species of longtooth and hybrid grouper. On the other hand, there was no interaction effect between concentration and species. Also, there was no interaction effect among species, concentration, and water temperature.

키워드

참고문헌

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