Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Anti-Inflammatory and Moisturizing Effect of Centella Extracts Fermented in Jeju Lava Water

제주용암해수로 숙성된 병풀 추출물의 항염 및 보습 효과

  • Lee, Jeesun (ABG North Asia Ltd / Global Discovery R&D Asia Innovation Center) ;
  • Myung, Cheol Hwan (Department of Genetic Engineering & Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Ji Eun (Department of Genetic Engineering & Graduate School of Biotechnology, Kyung Hee University) ;
  • Jo, Mi-Rae (DURAE Corporation/ Research & Development Center) ;
  • Kim, Hong-Suk (DURAE Corporation/ Research & Development Center) ;
  • Lee, Na-Young (DURAE Corporation/ Research & Development Center) ;
  • Woo, Heedong (Amway Korea Ltd/ Research & Development) ;
  • You, Jaeeun (Amway Korea Ltd/ Research & Development) ;
  • Jo, Hae (ABG North Asia Ltd / Global Discovery R&D Asia Innovation Center) ;
  • Hwang, Jae Sung (Department of Genetic Engineering & Graduate School of Biotechnology, Kyung Hee University)
  • 이지선 (에이비지노쓰아시아 (주) 글로벌 디스커버리 연구개발 아시아 이노베이션 센터) ;
  • 명철환 (경희대학교 생명공학원) ;
  • 이지은 (경희대학교 생명공학원) ;
  • 조미래 ((주)두래 기술연구소) ;
  • 김홍석 ((주)두래 기술연구소) ;
  • 이나영 ((주)두래 기술연구소) ;
  • 우희동 (한국암웨이 (주) 연구개발부) ;
  • 유재은 (한국암웨이 (주) 연구개발부) ;
  • 조해 (에이비지노쓰아시아 (주) 글로벌 디스커버리 연구개발 아시아 이노베이션 센터) ;
  • 황재성 (경희대학교 생명공학원)
  • Received : 2019.11.11
  • Accepted : 2019.12.03
  • Published : 2019.12.30
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Abstract

The leaves and stems of Centella asiatica have a long history of their usage as a medicine for the treatment of skin diseases such as ulcers and psoriasis, especially in Asia. Triterpenoids, the active components of Centella asiatica including asiaticoside, madecasosside, asiatic acid and madecassic acid, have shown to inhibit skin inflammation as well as improve skin photoaging. The main objective of this study is to investigate whether the Centella asiatica ripened with lava seawater which is rich in minerals known to be beneficial to human body can provide anti-inflammatory and moisturizing effects to skin. HPLC analysis showed that the concentration of triterpenoids increased further after ripening Centella asiatica with lava seawater. In order to confirm the inflammatory efficacy of the extract of the extract of the ripened Centella asiatica, the production of NO in LPS-activated RAW 264.7 cells and the expression of inflammatory cytokines in PM10 or UVB-induced HaCaT cells were observed. We found that the extract of the ripened Centella asiatica inhibited the expression of NO, IL-6, IL-8, and TNF-a and had higher inhibitory effect compared to the extract of the non-ripened Centella asiatica. In order to confirm the skin moisturizing effect, we investigated the synthesis of HA in HaCaT cells. The result showed HA production was enhanced in a concentration-dependent manner from the ripened group, while there was no efficacy from the non-ripened group. Taken together, it is concluded that the extract of the Centella asiatica ripened with lava seawater was effective in anti-inflammation and moisturization.

병풀의 잎과 줄기는 오랜 기간 궤양, 건선과 같은 피부질환 치료를 위한 약재로 사용되어왔으며, 병풀의 유효성분인 asiaticoside, madecasosside, asiatic acid, madecassic acid와 같은 triterpenoids는 피부 항염 및 피부 광 노화 개선 등의 효능이 밝혀져 있다. 본 연구의 목적은 인체에 유익한 미네랄을 많이 함유하고 있는 제주용암해수로 병풀을 숙성한 뒤 추출하여 피부 항염 및 보습 효능을 확인하는 것이다. 제주용암해수로 병풀을 숙성한 뒤 추출물을 HPLC에 분석하였을 때 triterpenoids의 농도가 더 증가하는 것을 관찰하였다. 숙성한 병풀 추출물의 항염증 효능을 확인하기 위해 LPS로 활성화된 RAW 264.7 세포에서 NO의 생성과 PM10 혹은 UV에 의해 유도된 HaCaT 세포에서 염증성 사이토카인들의 발현을 확인하였다. 실험 결과, 숙성한 병풀 추출물이 NO와 IL-6, IL-8, TNF-a의 발현을 저해하였고 숙성하지 않은 군에 비해 숙성한 군에서 억제 효능이 더 높은 것을 확인하였다. 또한, 피부 보습 효능을 확인하기 위해 HaCaT 세포에서 HA의 합성을 확인한 결과 숙성하지 않은 군에서는 효능이 없는 것에 반해 숙성한 군에서 농도 의존적으로 HA 생성이 증가하는 것을 확인하였다. 따라서 본 연구 결과를 바탕으로 제주용암해수로 숙성한 병풀 추출물은 항염과 보습에 유효한 소재임을 확인하였고 숙성하지 않은 병풀 추출물보다 효능이 우수할 것으로 판단된다.

Keywords

References

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