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A Comparison of Complete Blood Cell Count in Canine Blood Samples Obtained from the Jugular Vein, Cephalic Vein and Lateral Saphenous Vein

  • An, Hyung-Mo (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Song, Joong-Hyun (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • An, Su-Jin (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Yu, Do-Hyeon (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Young Joo (College of Veterinary Medicine, Western University of Health Sciences) ;
  • Han, Donghyun (Section of Veterinary Research and Academic Consulting, Veteran, Inc.) ;
  • Jung, Dong-In (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
  • Received : 2019.04.26
  • Accepted : 2019.11.28
  • Published : 2019.12.31

Abstract

The purpose of this study was to compare the results of complete blood cell count (CBC) of blood samples collected from the jugular vein, cephalic vein and lateral saphenous vein and to find out if there were clinically significant differences. Total of 40 dogs were tested. CBC tests were conducted with blood samples obtained from the jugular vein, cephalic vein and lateral saphenous vein and manual differential count was performed to accurately distinguish the white blood cell (WBC) types. The results were analyzed using Repeated Measures ANOVA and posthoc test was conducted using the least significant difference method. As a result, there was a statistically significant difference (P < 0.05) in the total WBC and monocyte count. The post-hoc test of total WBC counts revealed a significant difference between the jugular vein and cephalic vein, and the jugular vein and lateral saphenous vein. For monocyte counts, a significant difference was observed between the jugular vein and lateral saphenous vein.

Keywords

References

  1. Alleman AR. The effects of hemolysis and lipemia on serum biochemical constituents. Vet Med 1990; 85: 1272-1284.
  2. Berchelmann ML, Kalter SS, Britton HA. Comparison of hematologic values from peripheral blood of the ear and venous blood of infant baboons (Papio cynocephalus). Lab Anim Sci 1973; 23: 48-52.
  3. Daae LNW, Hallerud M, Halvorsen S. A comparison between haematological parameters in 'capillary' and venous blood samples from hospitalized children aged 3 months to 14 years. Scand J Clin Lab Invest 1991; 51: 651-654. https://doi.org/10.3109/00365519109104576
  4. Daae LNW, Halvorsen S, Mathisen PM, Mironska K. A comparison between haematological parameters in 'capillary' and venous blood from healthy adults. Scand J Clin Lab Invest 1988; 48: 723-726. https://doi.org/10.3109/00365518809085796
  5. Douglas JW, Harold T. The complete blood count, bone marrow examination, and blood banking. In: Small animal clinical diagnosis by laboratory methods,5th ed. Amsterdam: Elsevier. 2012: 12-37.
  6. Goldsmith HL, Spain S. Margination of leukocytes in blood flow through small tubes. Microvasc Res 1984; 27: 204-222. https://doi.org/10.1016/0026-2862(84)90054-2
  7. Ilkiw JE, Rose RJ, Martin ICA. A comparison of simultaneously collected arterial, mixed venous, jugular venous and cephalic venous blood samples in the assessment of blood gas and acid base status in the dog. J Vet Intern Med 1991; 5: 294-298. https://doi.org/10.1111/j.1939-1676.1991.tb03136.x
  8. Jensen AL, Iversen L, Petersen TK. Study on biological variability of haematological components in dogs. Comp Haematol Int 1998; 8: 202-204. https://doi.org/10.1007/BF02752849
  9. Jensen AL, Wenck A, Koch J, Poulsen JD. Comparison of results of haematological and clinical chemical analyses of blood samples obtained from the cephalic and external jugular veins in dogs. Res Vet Sci 1994; 56: 24-29. https://doi.org/10.1016/0034-5288(94)90191-0
  10. Kayiran SM, Ozbek N, Turan M, Gurakan B. Significant differences between capillary and venous complete blood counts in the neonatal period. Clin Lab Haematol 2003; 25: 9-16. https://doi.org/10.1046/j.1365-2257.2003.00484.x
  11. Kim MJ, Jin JH, Kwon YS, Jun YH, Kim SK. Comparison of blood counts in capillary and venous blood in children. Korean J Hematol 2009; 44: 237-243. https://doi.org/10.5045/kjh.2009.44.4.237
  12. Lippi G, Guidi GC, Mattiuzzi C, Plebani M. Preanalytical variability: the dark side of the moon in laboratory testing. Clin Chem Lab Med 2006; 44: 358-365. https://doi.org/10.1515/CCLM.2006.073
  13. Ozbek N, Gurakan B, Kayiran SM. Complete blood cell counts in capillary and venous blood of healthy term newborns. Acta Haematol 2000; 103: 226-228. https://doi.org/10.1159/000041056
  14. Parker BJ, Blowey RW. A comparison of blood from the jugular vein and coccygeal artery and vein of cows. Vet Rec 1974; 95: 14-18. https://doi.org/10.1136/vr.95.1.14
  15. Peevy KJ, Grant PH, Hoff CJ. Capillary venous differences in neonatal neutrophil values. Am J Dis Child 1982; 136: 357-358.
  16. Podgorski T, Bartkowiak U, Pawlak M. Comparison of hematological parameters of venous and capillary blood in athletes. Trends Sport Sci 2014; 21: 39-45.
  17. Schalk E, Heim MU, Koenigsmann M, Jentsch UK. Use of capillary blood count parameters in adults. Vox Sang 2007; 93: 348-353. https://doi.org/10.1111/j.1423-0410.2007.00978.x
  18. Wlliam JR, Florence MP, Arimando RIR. Design and methods used for preclinical hematotoxicity studies. In: Schalm's veterinary hematology, 6th ed. Hoboken: Willey-Blackwell. 2010: 71-77.
  19. Yang ZW, Yang SH, Chen L, Qu J, Zhu J, Tang Z. Comparison of blood counts in venous, fingertip and arterial blood and their measurement variation. Clin Lab Haematol 2001; 23: 155-159. https://doi.org/10.1046/j.1365-2257.2001.00388.x