Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Anti-inflammatory and Immunosuppressive Effects of Panax notoginseng

  • Cao, Thao Quyen (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University) ;
  • Han, Jae Hyuk (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University) ;
  • Lee, Hyun-Su (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University) ;
  • Ha, Manh Tuan (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University) ;
  • Woo, Mi Hee (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University) ;
  • Min, Byung Sun (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University)
  • Received : 2019.05.30
  • Accepted : 2019.09.18
  • Published : 2019.12.31
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Abstract

Here, we designed to examine the anti-inflammatory effects on RAW264.7 cells and the immunosuppressive effects by evaluating interleukin-2 (IL-2) production in Jurkat T cells using a MeOH extract of Panax notoginseng roots. The results showed that the MeOH extract inhibited the synthesis of nitric oxide (NO) in a dose-dependent manner (IC50 value of 7.08 ㎍/mL) and displayed effects on T cell activation at a concentration of 400 ㎍/mL. In efforts to identify the potent compounds, bioactivity-guided fractionation of the MeOH extract and chemical investigation of its active CH2Cl2-, EtOAc-, and butanol-soluble fractions led to the successful isolation and identification of eleven compounds, including two polyacetylenes (1, 2), a steroid saponin (3), seven dammarane-type ginsenosides (4 - 10), and an oleanane-type ginsenoside (11). Among them, compound 11 was isolated from this plant for the first time. Compound 2 exhibited potent inhibitory effects on NO synthesis and an immunosuppressive effect with IC50 values of 2.28 and 65.57 μM, respectively.

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References

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