Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of Lactobacillus plantarum subsp. plantarum Producing C30 Carotenoid 4,4'-Diaponeurosporene and the Assessment of Its Antioxidant Activity

  • Kim, Mibang (Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University) ;
  • Seo, Dong-Ho (Department of Food Science and Technology, College of Agriculture and Life Sciences, Jeonbuk National University) ;
  • Park, Young-Seo (Department of Food Science and Biotechnology, Gachon University) ;
  • Cha, In-Tae (Microorganism Resources Division, National Institute of Biological Resources) ;
  • Seo, Myung-Ji (Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University)
  • Received : 2019.09.05
  • Accepted : 2019.10.13
  • Published : 2019.12.28
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Abstract

Carotenoids are organic pigments with antioxidant properties and are widespread in nature. Here, we isolated five microbes, each forming yellow-colored colonies and harboring C30 carotenoid biosynthetic genes (crtM and crtN). Thereafter, Lactobacillus plantarum subsp. plantarum KCCP11226, which showed the highest carotenoid production, was finally selected and the produced pigment was identified as C30 carotenoid 4,4'-diaponeurosporene. This strain exhibited the highest survival rate under oxidative stress and its carotenoid production was also enhanced after exposure to 7 mM H2O2. Moreover, it showed the highest ability to scavenge DPPH free radical. Our results suggested that L. plantarum subsp. plantarum KCCP11226, which produces 4,4'-diaponeurosporene as a natural antioxidant, may be a functional probiotic.

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References

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