DOI QR코드

DOI QR Code

Zooshikella sp. 17TA 색소 추출물의 물리화학적 안정성과 항균활성

The Physicochemical Stabilities and Antimicrobial Activities of Pigment Extracts from Zooshikella sp. 17TA

  • 박재명 (한남대학교 생명시스템과학과) ;
  • 박진숙 (한남대학교 생명시스템과학과)
  • Park, Jae-Myeong (Department of Biological Science and Biotechnology, Hannam University) ;
  • Park, Jin-Sook (Department of Biological Science and Biotechnology, Hannam University)
  • 투고 : 2019.11.21
  • 심사 : 2019.12.16
  • 발행 : 2019.12.31

초록

In this study, the stability of the extracted natural pigments against light, temperature, pH, metal ions, and antimicrobial activity were evaluated in marine bacteria Zooshikella sp. 17TA. The pigment of the strain used in the study was red with maximum absorption at a wavelength of 541 nm. The stability of the pigment was evaluated by measuring the absorbance while preserving for 15 days and examining the retention rate. After 15 days of irradiation, the pigment of this bacterium showed 98% retention in the dark and 91% retention in the temperature range of -20℃ ~ 30℃. When the pH was in the range 4-7, the retention was about 80%, and the retention rate was higher than 85% for all kinds of metal ions except for CuCl2, ZnCl2, and KCl. The bacterial pigments showed high stability under the given irradiated pH, temperature, and metal ion conditions and had shown activity against gram-positive strains. These results suggest that this highly conserved microbial pigment can be applied to the food industry.

키워드

참고문헌

  1. Park, J. S., Cho, H. H. and Kang, M. H. 2009. The Physicochemical stabilities and biological activities of pigment extracts from Pseudoalteromonas sp. Ju11-1 and Pseudoalteromonas sp. Ju14. Korean J. Microbiol. 45, 404-410.
  2. Ko, M. H. and Kim, J. W. 2011. Seoul Elementary school students perception and information needs on artificial food colorants. Korean J. Food Cook. Sci. 27, 643-651. https://doi.org/10.9724/kfcs.2011.27.6.643
  3. Boo, H. O., Shin, J. S., Hwang, S. J., Bae, C. S. and Park, S. H. 2012. Antimicrobial effects and antioxidative activities of the cosmetic composition having natural plant pigments. Korean J. Plant Res. 25, 80-88. https://doi.org/10.7732/kjpr.2012.25.1.080
  4. Jeong, D. W. and Park, J. S. 2008. Characterization of pigment-producing Pseudoalteromonas spp. from marine habitats and their optimal conditions for pigment production. J. Life Sci. 18, 1752-1757. https://doi.org/10.5352/JLS.2008.18.12.1752
  5. Yong, H. Y. and Park, J. S. 2004. Diversity of pigment-producing halophilic bacteria isolated from coastal seawater and solar saltern in Korea. Korean J. Microbiol. 40, 302-306.
  6. Jeon, C. P., Lee, J. B. and Kwon, G. S. 2013. Stability of Monascus pigment produced by Monascus sp. MK2. J. Life Sci. 23, 669-675. https://doi.org/10.5352/JLS.2013.23.5.669
  7. Boo, H. O., Hwang, S. J., Bae, C. S., Park, S. H. and Song, W. S. 2011. Antioxidant activity according to each kind of natural plant pigments. Korean J. Plant Res. 24, 105-112. https://doi.org/10.7732/kjpr.2011.24.1.105
  8. Tak, E. S., Bae, M. W., Kang, S. H., Bae, Y. J., Kang, S. Y., Jung, S. K. and Hong, I. K. 2010. Presentation of target color with extraction variables in natural dyes extraction. Appl. Chem. Eng. 21, 559-563.
  9. Cho, G. S. 2018. Production of edible paints using to the Monascus spp. pigments. Kwang Jang, 219, pp 271-291.
  10. Seo, Y. 1E., Jung, H. J., Hong, S. M. and Yu, T. S. 2007. Optimization of production of pigment from Monascus sp. in liquid culture. Korean J. Microbiol. 43, 59-65.
  11. Won, N. I., Lee, G. E., Ko, K., Oh, D. C., Na, Y. H. and Park, J. S. 2017. Identification of a bioactive compound, violacein, from Microbulbifer sp. isolated from a marine sponge Hymeniacidon sinapium on the west coast of Korea. Microbiol. Biotechnol. Lett. 45, 124-132. https://doi.org/10.4014/mbl.1702.02002
  12. Park, C. D., Jung, H. J. and Yu, T. S. 2005. Isolation of pigment overproducing mutant from Monascus purpureus and optimization of pigment production. Korean J. Microbiol. 41, 130-134.
  13. Lee, G. E. and Park, J. S. 2017. Dyeing effects of natural pigment from marine bacterium, Microbulbifer sp. PPB12. J. Korea Acad. Industr. Coop. Soc. 18, 527-533.
  14. Shin, Y., Kang, C. H. and So, J. S. 2016. Red pigment producing Serratia marcescens isolated from abalone (Haliotis discus). KSBB Journal, 31, 214-218. https://doi.org/10.7841/ksbbj.2016.31.4.214
  15. Choi, J. M. and Kim, Y. S. 2009. Dyeing properties of microbial violacein on mutifiber fabrics. Fashion and Text. Res. J. 11, 818-826.
  16. Kim, J. S., Kim, M. C., Lee, K. J. and Heo, M. S. 2009. Isolation and optimal culture conditions of prodigiosin-like pigment produced by Zooshikella sp. JE-34. Microbiol. Biotechnol. Lett. 37, 219-225.
  17. Kim, Y. S. and Choi, J. M. 2011. Physicochemical and dyeing properties of microbial prodiginine from Zooshikella sp. J. Korean Soc. Cloth. Text. 35, 431-441. https://doi.org/10.5850/JKSCT.2011.35.4.431
  18. Park, H. A. 2019. Absorption Spectroscopic studies of prodigiosin extracted from Serratia marcescens strain. Appl. Sci. Converg. Technol. 36, 355-361.
  19. Lee, Y. R., Mitchell, R. J. and Whang, K. S. 2016. Isolation and characterization of antifungal violacein producing bacterium Collimonas sp. DEC-B5. Korean J. Microbiol. 52, 212-219. https://doi.org/10.7845/kjm.2016.6031
  20. Choi, J. M., Kim, Y. S. and Yi, E. J. 2010. Colorimetric properties and color sensibility factors for naturally dyed fabrics by microbial prodiginine colorant. Korean J. Sci. Emot. Sensib. 13, 693-702.
  21. Kim, Y. S. and Park, J. S. 2010. Characterization of pigment-producing Kocuria sp. K70 and the optimal conditions for pigment production and physical stability. KSBB Journal, 25, 513-519.
  22. Kim, Y. H. and Lee, S. S. 1993. Yellow pigment from Rhodospirillum rubrum by acetone extraction. J. Korean Soc. Food Nutr. 6, 322-328.
  23. Hearn, W. R., Medina, J. and Elson, M. K. 1968. Colour change of prodigiosin. Nature, 220, 170-171. https://doi.org/10.1038/220170a0
  24. Park, J. S., Jeong, D. W. and Kang, M. H. 2009. The physicochemical stabilities and biological activities of pigment extract from marine bacterium Pseudoalteromonas psicida TA20. J. Life Sci. 19, 1132-1138. https://doi.org/10.5352/JLS.2009.19.8.1132
  25. Kim, J. S., Kim, M. C., Harikrishnan, R., Han, Y. J. and Heo, M. S. 2009. Taxonomical characterization and antimicrobial activity of red pigment-producing marine bacterium strain JE-34. Korean J. Microbiol. 45, 368-376.
  26. Ji, K. and Kim, Y. T. 2019. Antimicrobial activity of prodigiosin from Serratia sp. PDGS 120915 against intestinal pathogenic bacteria. Microbiol. Biotechnol. Lett. 47, 459-464. https://doi.org/10.4014/mbl.1901.01006