Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Effect of Cold Storage on Parasitism and Survival of Aphidius colemani and Meteorus pulchricornis

저온저장이 기생성 천적인 콜레마니진디벌과 예쁜가는배고치벌의 기생 및 생존에 미치는 영향

  • Seo, Meeja (Crop Protection Division, Departments of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, RDA) ;
  • Kim, Jeong Hwan (Crop Protection Division, Departments of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, RDA) ;
  • Seo, Bo Yoon (Crop Protection Division, Departments of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, RDA) ;
  • Park, Hong Hyun (Crop Protection Division, Departments of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, RDA) ;
  • Ji, Chang Woo (Crop Protection Division, Departments of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, RDA) ;
  • Park, Bueyong (Crop Protection Division, Departments of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, RDA) ;
  • Lee, Sang Gu (Crop Protection Division, Departments of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, RDA) ;
  • Cho, Jum Rae (Crop Protection Division, Departments of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, RDA)
  • 서미자 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 김정환 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 서보윤 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 박홍현 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 지창우 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 박부용 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 이상구 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 조점래 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과)
  • Received : 2019.09.27
  • Accepted : 2019.11.19
  • Published : 2019.12.01
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Abstract

When Aphidius colemani mummies were stored at four different temperatures (6, 8, 10 and 12 ± 1℃), they could be stored at 8℃ for 10 days with over 50% adult emergence. A number of mummies of Rhopalosiphum padi parasitized by A. colemani adults emerged from mummies after 8℃ storage clearly reduced with increasing duration of storage and especially declined with storage longer than 13 days showing < 20% parasitism. However, there were no differences in aphid parasitisms by A. colemani on 3 to 10 days after storage. Over 3 day storage at 8℃ was adversely affected on survival of A. colemani adults. Therefore, cold storage of A. colemani adults is considered as an unsuitable method for mass production of biological control agent. When 2-day-old Meteorus pulchricornis cocoons were stored at 6, 8 and 10 ± 1℃, it was possible to be stored up to 63 days at 8℃, showing the highest emergence. With increasing of cold storage duration at 8℃, parasitism on 2nd and 3rd larval instar of Spodoptera litura by stored M. pulchricornis significantly reduced. However, parasitic rate by M. pulchricornis stored for 2 weeks was similar to that by 1 week stored ones. Therefore, M. pulchricornis cocoons could be stored up to 2 weeks at 8℃ for stockpiling before release.

콜레마니진디벌 머미는 처리한 온도조건(6, 8, 10 및 12 ± 1℃) 중, 8℃에서 저장 10일 까지 50% 이상의 우화율을 보였다. 머미 우화율이 가장 높았던 8℃에서 콜레마니진디벌 머미의 저장기간에 따른 기생된 기장테두리진딧물 머미 수를 조사한 결과, 저장 후 진딧물 기생율이 급격히 떨어지는 것이 확인되었으며, 13일 이상의 저장할 경우 평균 기생율이 20%를 넘지 못하였다. 머미 상태로 저장기간 3일부터 10일까지는 기생율의 차이를 보이지 않았다. 콜레마니진디벌 성충은 3일 이상의 저장은 생존율이 급격히 감소하는 경향을 보여 성충태로의 저온저장은 불가능할 것으로 판단되었다. 고치 형성 후 2일차 된 예쁜가는배고치벌 고치를 6, 8 및 10 ± 1℃ 에서의 저장 기간에 따른 우화율을 조사한 결과, 8℃에서 가장 높은 우화율을 나타냈으며 최대 63일까지 저장이 가능하였다. 8℃ 조건에서 저장기간이 길어질수록 산란기주인 담배거세미나방 2, 3령 유충의 치사율과 고치 형성 수가 급격히 감소하는 경향이었으며, 최적 저장기간은 2주 정도인 것으로 판단되었다.

Keywords

References

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