한국응용곤충학회지 (Korean journal of applied entomology)

  • 제58권4호
  • /
  • Pages.283-289
  • /
  • 2019
  • /
  • 1225-0171(pISSN)
  • /
  • 2287-545X(eISSN)
한국응용곤충학회 (Korean Society of Applied Entomology)
권호 표지
DOI QR코드

DOI QR Code

동애등애유충에서 분리된 펩타이드의 신장에서의 폐렴간균 감염 억제 효능

Peptide isolated from Hermetia illucens larvae inhibits mice from Klebsiella pneumoniae infection in the kidney

  • 이동훈 (경희대학교 일반대학원 기초의과학과) ;
  • 주기백 (경희대학교 일반대학원 기초의과학과) ;
  • 강해지 (경희대학교 일반대학원 기초의과학과) ;
  • 이수화 (경희대학교 일반대학원 기초의과학과) ;
  • 전복실 (경희대학교 의과대학 의동물학교실)
  • Lee, Dong-Hun (Department of Biomedical Science, Graduate School, Kyung Hee University) ;
  • Chu, Ki-Back (Department of Biomedical Science, Graduate School, Kyung Hee University) ;
  • Kang, Hae-Ji (Department of Biomedical Science, Graduate School, Kyung Hee University) ;
  • Lee, Su-Hwa (Department of Biomedical Science, Graduate School, Kyung Hee University) ;
  • Quan, Fu-Shi (Department of Medical Zoology, Kyung Hee University School of Medicine)
  • 투고 : 2019.10.06
  • 심사 : 2019.11.07
  • 발행 : 2019.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

항생제의 오남용은 세균의 항생제 내성을 증가시켜 세균감염에 의한 질병 치료에 어려움을 초래한다. 본 연구에서는 동애등애유충으로부터 분리된 펩타이드의 신장에서의 폐렴간균 감염 억제 효능을 관찰하였다. 마우스는 비강을 통해 폐렴간균을 감염시키고 1일 후 펩타이드를 마우스에 근육 주사로 투여하였다. 10일 후 마우스를 희생하여 신장에서 세균 감염증을 조사하였다. 대조군에 비해 펩티드를 투여한 마우스의 신장에서 세균 감염증상, 몸무게의 감소가 유의하게 억제되었고 생존률이 농도 의존적으로 증가하는 것으로 나타났다. 이러한 결과들은 동애등애로부터 분리된 펩타이드가 폐렴 간균의 신장에서의 감염증상을 억제할 수 있음을 보여준다. 따라서 동애등애로부터 분리된 펩타이드는 효과적인 항생제 개발에서 가능성 높은 후보물질이 될 수 있을 것이다.

Overuse of antibiotics has significantly contributed to an increase in microbial antibiotic resistance, causing difficulties in the suppression of microbe-borne infectious diseases. In this study, we determined the anti-Klebsiella pneumoniae effect in the kidneys of mice induced by peptides isolated from H. illucens larvae. Mice were intranasally infected with a high dose of K. pneumoniae and 1 day later, peptides were introduced through the intramuscular route. Mice were sacrificed on day 10 upon K. pneumoniae infection to determine the bacterial loads in the kidneys. Mice receiving peptide treatment demonstrated significantly reduced bacterial loads, reduced bodyweight loss, and higher survival in a dose-dependent manner compared to control. These results indicate that peptide isolated from H. illucens larva inhibits K. pneumoniae infection in the kidney. The peptide from H. illucens larva could be a potential candidate for the development of an effective antibacterial drug.

키워드

참고문헌

  1. Choi, W.H., Yun, J.H., Chu, J.P., Chu, K.B., 2012. Antibacterial effect of extracts of Hermetia illucens (Diptera: Stratiomyidae) larvae against gram-negative bacteria. Entomol. Res. 42, 219-226. https://doi.org/10.1111/j.1748-5967.2012.00465.x
  2. Choi, W.H., Choi, H.J., Goo, T.W., Quan, F.S., 2018. Novel antibacterial peptides induced by probiotics in Hermetia illucens (Diptera: Stratiomyidae) larvae. Entomol. Res. 48, 237-247. https://doi.org/10.1111/1748-5967.12259
  3. Chu, K.B., Jeon, G.C., Quan, F.S., 2014. Hexanedioic acid from Hermetia illucens larvae (Diptera: Stratiomyidae) protects mice against Klebsiella pneumoniae infection. Entomol. Res. 44, 1-8. https://doi.org/10.1111/1748-5967.12043
  4. De Smet, J., Wynants, E., Cos, P., Van Campenhout, L., 2018. Microbial community dynamics during rearing of black soldier fly larvae (Hermetia illucens) and impact on exploitation potential. Appl. Environ. Microbiol. 84.
  5. Elhag, O., Zhou, D., Song, Q., Soomro, A.A., Cai, M., Zheng, L., Yu, Z., Zhang, J., 2017. Screening, expression, purification and functional characterization of novel antimicrobial peptide genes from Hermetia illucens (L.). PLoS One 12, e0169582. https://doi.org/10.1371/journal.pone.0169582
  6. Erickson, M.C., Islam, M., Sheppard, C., Liao, J., Doyle, M.P., 2004. Reduction of escherichia coli O157:H7 and salmonella enterica serovar enteritidis in chicken manure by larvae of the black soldier fly. J. Food. Prot. 67, 685-690. https://doi.org/10.4315/0362-028X-67.4.685
  7. Fabbri, G., Panico, M., Dallolio, L., Suzzi, R., Ciccia, M., Sandri, F., Farruggia, P., 2013. Outbreak of ampicillin/piperacillin-resistant Klebsiella pneumoniae in a neonatal intensive care unit (NICU): investigation and control measures. Int. J. Environ. Res. Public Health 10, 808-815. https://doi.org/10.3390/ijerph10030808
  8. Forrest, J.W., Jr., John, F., Mills, L.R., Buxton, T.B., Moore, W.L., Jr., Hudson, J.B., Ozawa, T., 1977. Immune complex glomerulonephritis associated with Klebsiella pneumoniae infection. Clin. Nephrol. 7, 76-80.
  9. Kamaruzzaman, N.F., Tan, L.P., Hamdan, R.H., Choong, S.S., Wong, W.K., Gibson, A.J., Chivu, A., Pina, M.F., 2019. Antimicrobial polymers: the potential replacement of existing antibiotics?. Int. J. Mol. Sci. 20, 2747. https://doi.org/10.3390/ijms20112747
  10. Lalander, C., Diener, S., Magri, M.E., Zurbrugg, C., Lindstrom, A., Vinneras, B., 2013. Faecal sludge management with the larvae of the black soldier fly (Hermetia illucens)--from a hygiene aspect. Sci. Total. Environ. 458-460, 312-318. https://doi.org/10.1016/j.scitotenv.2013.04.033
  11. Li, Z., Mao, R., Teng, D., Hao, Y., Chen, H., Wang, X., Wang, X., Yang, N., Wang, J., 2017. Antibacterial and immunomodulatory activities of insect defensins-DLP2 and DLP4 against multidrug-resistant Staphylococcus aureus. Sci. Rep. 7, 12124. https://doi.org/10.1038/s41598-017-10839-4
  12. Liu, Q., Tomberlin, J.K., Brady, J.A., Sanford, M.R., Yu, Z., 2008. Black soldier fly (Diptera: Stratiomyidae) larvae reduce escherichia coli in dairy manure. Environ. Entomol. 37, 1525-1530. https://doi.org/10.1603/0046-225X-37.6.1525
  13. Muller, A., Wolf, D., Gutzeit, H.O., 2017. The black soldier fly, Hermetia illucens - a promising source for sustainable production of proteins, lipids and bioactive substances. Z. Naturforsch. C. 72, 351-363. https://doi.org/10.1515/znc-2017-0030
  14. Nasr, S.H., Radhakrishnan, J., D'Agati, V.D., 2013. Bacterial infection-related glomerulonephritis in adults. Kidney Int. 83, 792-803. https://doi.org/10.1038/ki.2012.407
  15. Santajit, S., Indrawattana, N., 2016. Mechanisms of antimicrobial resistance in eskape pathogens. Biomed. Res. Int. 2016, 2475067.
  16. Sharma, V.K., Johnson, N., Cizmas, L., McDonald, T.J., Kim, H., 2016. A review of the influence of treatment strategies on antibiotic resistant bacteria and antibiotic resistance genes. Chemosphere 150, 702-714. https://doi.org/10.1016/j.chemosphere.2015.12.084
  17. Shin, H.S., Park, S.I., 2019. Novel attacin from Hermetia illucens: cDNA cloning, characterization, and antibacterial properties. Prep. Biochem. Biotechnol. 49, 279-285. https://doi.org/10.1080/10826068.2018.1541807
  18. Shon, A.S., Bajwa, R.P., Russo, T.A., 2013. Hypervirulent (hypermucoviscous) Klebsiella pneumoniae: a new and dangerous breed. Virulence 4, 107-118. https://doi.org/10.4161/viru.22718
  19. Vogel, H., Muller, A., Heckel, D.G., Gutzeit, H., Vilcinskas, A., 2018. Nutritional immunology: Diversification and diet-dependent expression of antimicrobial peptides in the black soldier fly Hermetia illucens. Dev. Comp. Immunol. 78, 141-148. https://doi.org/10.1016/j.dci.2017.09.008
  20. WHO, 2018. High levels of antibiotic resistance found worldwide, new data shows (Geneva, Switzerland). (https://www.who.int/mediacentre/news/releases/2018/antibiotic-resistance-found/en/) (accessed August 2019).
  21. Yi, H.Y., Chowdhury, M., Huang, Y.D., Yu, X.Q., 2014. Insect antimicrobial peptides and their applications. Appl. Microbiol. Biotechnol. 98, 5807-5822. https://doi.org/10.1007/s00253-014-5792-6