Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Peptide isolated from Hermetia illucens larvae inhibits mice from Klebsiella pneumoniae infection in the kidney

동애등애유충에서 분리된 펩타이드의 신장에서의 폐렴간균 감염 억제 효능

  • Lee, Dong-Hun (Department of Biomedical Science, Graduate School, Kyung Hee University) ;
  • Chu, Ki-Back (Department of Biomedical Science, Graduate School, Kyung Hee University) ;
  • Kang, Hae-Ji (Department of Biomedical Science, Graduate School, Kyung Hee University) ;
  • Lee, Su-Hwa (Department of Biomedical Science, Graduate School, Kyung Hee University) ;
  • Quan, Fu-Shi (Department of Medical Zoology, Kyung Hee University School of Medicine)
  • 이동훈 (경희대학교 일반대학원 기초의과학과) ;
  • 주기백 (경희대학교 일반대학원 기초의과학과) ;
  • 강해지 (경희대학교 일반대학원 기초의과학과) ;
  • 이수화 (경희대학교 일반대학원 기초의과학과) ;
  • 전복실 (경희대학교 의과대학 의동물학교실)
  • Received : 2019.10.06
  • Accepted : 2019.11.07
  • Published : 2019.12.01
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Abstract

Overuse of antibiotics has significantly contributed to an increase in microbial antibiotic resistance, causing difficulties in the suppression of microbe-borne infectious diseases. In this study, we determined the anti-Klebsiella pneumoniae effect in the kidneys of mice induced by peptides isolated from H. illucens larvae. Mice were intranasally infected with a high dose of K. pneumoniae and 1 day later, peptides were introduced through the intramuscular route. Mice were sacrificed on day 10 upon K. pneumoniae infection to determine the bacterial loads in the kidneys. Mice receiving peptide treatment demonstrated significantly reduced bacterial loads, reduced bodyweight loss, and higher survival in a dose-dependent manner compared to control. These results indicate that peptide isolated from H. illucens larva inhibits K. pneumoniae infection in the kidney. The peptide from H. illucens larva could be a potential candidate for the development of an effective antibacterial drug.

항생제의 오남용은 세균의 항생제 내성을 증가시켜 세균감염에 의한 질병 치료에 어려움을 초래한다. 본 연구에서는 동애등애유충으로부터 분리된 펩타이드의 신장에서의 폐렴간균 감염 억제 효능을 관찰하였다. 마우스는 비강을 통해 폐렴간균을 감염시키고 1일 후 펩타이드를 마우스에 근육 주사로 투여하였다. 10일 후 마우스를 희생하여 신장에서 세균 감염증을 조사하였다. 대조군에 비해 펩티드를 투여한 마우스의 신장에서 세균 감염증상, 몸무게의 감소가 유의하게 억제되었고 생존률이 농도 의존적으로 증가하는 것으로 나타났다. 이러한 결과들은 동애등애로부터 분리된 펩타이드가 폐렴 간균의 신장에서의 감염증상을 억제할 수 있음을 보여준다. 따라서 동애등애로부터 분리된 펩타이드는 효과적인 항생제 개발에서 가능성 높은 후보물질이 될 수 있을 것이다.

Keywords

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