Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Current status of CRISPR/Cas9 base editor technologies and their applications in crop precision breeding

  • Kim, Rigyeong (Metabolic Engineering Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Song, Jaeeun (Metabolic Engineering Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Ga, Eunji (Metabolic Engineering Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Min, Myung Ki (Metabolic Engineering Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Lee, Jong-Yeol (Metabolic Engineering Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Lim, Sun-Hyung (Metabolic Engineering Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Beom-Gi (Metabolic Engineering Division, National Institute of Agricultural Sciences, Rural Development Administration)
  • Received : 2019.09.10
  • Accepted : 2019.10.22
  • Published : 2019.12.31
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Abstract

Plant biotechnologists have long dreamed of technologies to manipulate genes in plants at will. This dream has come true partly through the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology, which now has been used to edit genes in several important crops. However, there are many restrictions in editing a gene precisely using the CRISPR/Cas9 technology because CRISPR/Cas9 may cause deletions or additions in some regions of the target gene. Several other technologies have been developed for gene targeting and precision editing. Among these, base editors might be the most practically and efficiently used compared to others. Base editors are tools which are able to cause a transition from cytosine into thymine, or from adenine into guanine very precisely on specific sequences. Cytosine base editors basically consist of nCas9, cytosine deaminase, and uracil DNA glycosylase inhibitor (UGI). Adenine base editors consist of nCas9 and adenine deaminase. These were first developed for human cells and have since also been applied successfully to crops. Base editors have been successfully applied for productivity improvement, fortification and herbicide resistance of crops. Thus, base editor technologies start to open a new era for precision gene editing or breeding in crops and might result in revolutionary changes in crop breeding and biotechnology.

Keywords

References

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